EMAGE mouse gene expression spatial database

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EMAGE:10620

Fam136a family with sequence similarity 136, member A ( MGI:1913738)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003220. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003220_01	euxassay_003220_02	euxassay_003220_03	euxassay_003220_04

euxassay_003220_05	euxassay_003220_06	euxassay_003220_07	euxassay_003220_08	euxassay_003220_09

euxassay_003220_10	euxassay_003220_11	euxassay_003220_12	euxassay_003220_13	euxassay_003220_14

euxassay_003220_15	euxassay_003220_16	euxassay_003220_17	euxassay_003220_18	euxassay_003220_19

euxassay_003220_20	euxassay_003220_21	euxassay_003220_22	euxassay_003220_23	euxassay_003220_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10620_wholemount_strong.wlz

10620_wholemount_moderate.wlz

10620_wholemount_weak.wlz

10620_wholemount_possible.wlz

10620_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10620_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 03 04 05 06 07 19 20 21 22 23

adrenal gland

weak

homogeneous

weak expression: see section 07 08 09 10 16 17 18 19

thymus primordium

moderate

homogeneous

moderate expression: see section 11 12 13 14 15 16

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 10 17 18 19 20 21

pancreas

moderate

regional

moderate expression: see section 07 08 09 10 11 14 15 16 17

vibrissa

weak

regional

weak expression: see section 07 08 22

dorsal root ganglion

weak

regional

weak expression: see section 08 09 10 11 15 16 17

stomach

moderate

regional

moderate expression: see section 05 06 07 08 09 weak expression: see section 01 02 03 04

hindgut

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19 20

rectum

moderate

regional

moderate expression: see section 13

midgut

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21

meckel's cartilage

moderate

regional

moderate expression: see section 01 02 03 23 24

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17 18

upper jaw incisor

moderate

regional

moderate expression: see section 12 13 14 17 18

renal cortex

weak

regional

weak expression: see section 20

maturing glomerular tuft

weak

regional

weak expression: see section 05 06 07 08 09 10 16 17 18 19 20 21

left lung

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 weak expression: see section 12

right lung

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23 weak expression: see section 24

trachea

moderate

regional

moderate expression: see section 13 14

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4891
Entity Detected:	Fam136a, family with sequence similarity 136, member A ( MGI:1913738)
Sequence:	sense strand is shown >T4891 GCGCAGCGGGTGCACACGCGGGCGCCGGCGCGGCTGAGCTCAGGGTCGCCATGGCGGAGGTGCAGCAACT CCGAGTGCAGGAGGCTGTGGACGCCATGGTGAAAAGTGTGGAGAGAGAGAACATCCGGAAGATGCAGGGC CTCATGTTCCGGTGCAGCGCCAACTGCTGTGAAGACACCCAAGCATCTATGCAGCAGGTGCACCAATGCA TCGAGCGCTGCCATGCGCCTCTGGCTCAAGCCCAGGCTCTGGTCACCAGCGAGCTGGAAAGGTTCCAGGA CCGCCTGGCCCGCTGCACCATGCATTGCAATGACAAAGCCAAAGACTCAATGGATGCAGGAACTAAGGAG CTTCAGGTGAAGCGACAGCTAGACAGCTGTGTGACCAAGTGTGTGGATGACCACATGCACCTCATCCCAA CAATGACCAAGAAGATGAAGGAGTCTCTGTCATCCATAGGGAAATAAAATTGTTTGCCAGTGACCATCGG GACTGCGGGCAGGCTATTTAAAAGGAAGAATGGAAGTTTAATATGTTAAGCAATGTTTACGAATGAGGAA AACTTAGACACAGCAAGTTCCAGGCAAATGCTGCTCACTGCTGGACACTAGTCCCTTTACATTCGGATCT TAGAAGGTGAAGTAGGAAGAAGCTGGTGCTAAGATTTGGATCACAGATAGCTCAGAGGAGAAGTCTAGAT CCTAAATATTCATATGCAAGTGTCTGTACTGTAGTAGAGATCAACCTTGTACCAAGAGCTGAGTTTCTTA CTACAAACATCCAGGCTGGCAGCTCTCTTCAACCTGCTGGTAAAATCTGAGGATCGGTGCATCTCCTCAG AGAAGCTTGACAAATCCTGTTGACCAGAAGTATCATCACACCACCTTGCATATGCTGGGAAACAAAACCC AGTAGGCTAGGAATTCAGAGGAAAGGATTTCCTTTTCAAATCTATATTTTGCTTAGTTTTGTCTTATATG CAGGGCACACTGGATTTATGATACAGGAGGGAAAGTGGTTTGAGTCACTTCTAAAGGTTCCTAGTATTAT GTACCGTCAAGATGGGAATGTCCATTTCTTCAAATTCTGTTACTCTAGAAGCCCCTGAAGAATCCGGACA GTGTCACACAGTGAACATGGGAAACAGCCTTCTTCACTGACTTGTGCAGCAAAGTGACACATCCTTATGA AAAGCAAGGGGGTAGGCTGTCACTCACATGCCAGTCGCTAAGAATAAGCAGTAACTAGGAATTATTGAGA AGTGCAAACCCAGTATTAATCAGCTCTGAATCTACAGAGCCTTATAGCAACAAGAAAGCCAGAAACTGAG CAACTTGGGCCACAGATGGGTAGGACCACTGTAGAACAAGAGAGGCACACACTTTCTTGCCAAATGCCAT TTTTGCCTGGGTGGTTTTTATTTTTTATTTTCTTTGCAGTGCAGTGTTGCTGGCCCAACATAGCACCTTA TCTTTTGGATAAGTACTATGTTGTATTTATACCAATTAATTACTTGAAACAGTGATACATCTGTCTTTTT GCTGTGTCAAGCCTGCAATATATTTTCCAAGTGGTTTGGTTTTTAAATAAAGTTTCATTGTCTCCAAAAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5100000 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5100000 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10618 same embryo
	EMAGE:10619 same embryo
	EurExpress:euxassay_003220 same experiment
	MGI:4824709 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source