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EMAGE:10625

Cd97 CD97 antigen ( MGI:1347095)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10625 EMAGE:10625 EMAGE:10625 EMAGE:10625 EMAGE:10625
"Pseudo-wholemount" of euxassay_000843. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_000843_01 euxassay_000843_02 euxassay_000843_03 euxassay_000843_04
EMAGE:10625 EMAGE:10625 EMAGE:10625 EMAGE:10625 EMAGE:10625
euxassay_000843_05 euxassay_000843_06 euxassay_000843_07 euxassay_000843_08 euxassay_000843_09
EMAGE:10625 EMAGE:10625 EMAGE:10625 EMAGE:10625 EMAGE:10625
euxassay_000843_10 euxassay_000843_11 euxassay_000843_12 euxassay_000843_13 euxassay_000843_14
EMAGE:10625 EMAGE:10625 EMAGE:10625 EMAGE:10625 EMAGE:10625
euxassay_000843_15 euxassay_000843_16 euxassay_000843_17 euxassay_000843_18 euxassay_000843_19
EMAGE:10625 EMAGE:10625 EMAGE:10625 EMAGE:10625 EMAGE:10625
euxassay_000843_20 euxassay_000843_21 euxassay_000843_22 euxassay_000843_23 euxassay_000843_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10625Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10625_wholemount_strong.wlz
10625_wholemount_moderate.wlz
10625_wholemount_weak.wlz
10625_wholemount_possible.wlz
10625_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10625_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
foregut-midgut junction
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16 17 18
not examined not examined
regionalnot examined expression: see section 24
stomach
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17 18 19 20 weak expression: see section 04 05 06 07 08 09 10 11
midgut
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18
not examined not examined
regionalnot examined expression: see section 08 09 10 11 12 13 14 15 16 17 18
skeleton
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 23 24
stomach pyloric region lumen
moderate moderate
homogeneousmoderate expression: see section 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2298
Entity Detected:Cd97, CD97 antigen ( MGI:1347095)
Sequence:sense strand is shown

>T2298
TGNCCCTCNAGCCAGATTCGGATCCTTGATCCTATTCCTGGTCGGCGTGGAGAATGAAGGGGGTGAGGTG
GGCCTGCGCTGCCGCCTGGTGGCCATGATGCTGCATTTCTGCTTCCTGGCCGCCTTCTGCTGGATGGCAC
TGGAGGGCGTGGAGCTCTACTTCCTGGTGGTGCGTGTGTTCCAGGGCCAAGGCCTGAGTACATGGCAACG
CTGCCTGATTGGCTACGGGGTGCCCCTCCTCATCGTGGCCATCTCAATGGCAGTTGTCAAAATGGATGGC
TATGGGCATGCAACATACTGCTGGCTGGACTTTAGGAAGCAGGGCTTTCTCTGGAGCTTTTCGGGACCCN
TGGCCTTCATCATTTTCTGCAATGCTGCCATTTTCGTGATCACTGTCTGGAAGCTCACAAAGAAGTTTTC
TGAAATCAACCCAAACATGAAGAAGTTAAGGAAGGCGAGGGTGCTGACCATCACCGCCATCGCCCAGCTC
CTTGTGCTGGGCTGCANCTGGGGCTTCGGCCTGTTCCTCTTCAACCCCCATAGCACATGGCTATCC
Notes:The probe template was PCR amplified from IMAGE:1122591 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1122591 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10622 same embryo
 EMAGE:10624 same embryo
 EMAGE:10621 same embryo
 EMAGE:10626 same embryo
 EMAGE:10623 same embryo
 EurExpress:euxassay_000843 same experiment
 MGI:4823748 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS