EMAGE mouse gene expression spatial database

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EMAGE:10634

Rab31 RAB31, member RAS oncogene family ( MGI:1914603)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003248. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003248_01	euxassay_003248_02	euxassay_003248_03	euxassay_003248_04

euxassay_003248_05	euxassay_003248_06	euxassay_003248_07	euxassay_003248_08	euxassay_003248_09

euxassay_003248_10	euxassay_003248_11	euxassay_003248_12	euxassay_003248_13	euxassay_003248_14

euxassay_003248_15	euxassay_003248_16	euxassay_003248_17	euxassay_003248_18	euxassay_003248_19

euxassay_003248_20	euxassay_003248_21	euxassay_003248_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10634_wholemount_strong.wlz

10634_wholemount_moderate.wlz

10634_wholemount_weak.wlz

10634_wholemount_possible.wlz

10634_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10634_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 06 07 08 weak expression: see section 22

submandibular gland primordium

moderate

regional

moderate expression: see section 19 weak expression: see section 11 12 13 20 21 22

vibrissa

moderate

regional

moderate expression: see section 09 10 22 weak expression: see section 11 21

medulla oblongata basal plate

moderate

regional

moderate expression: see section 11 12 weak expression: see section 10

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 09 10 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 11 18

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 13 weak expression: see section 18

cervical ganglion

moderate

regional

moderate expression: see section 12 weak expression: see section 18

thoracic ganglion

weak

regional

weak expression: see section 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 13 17 18 19 weak expression: see section 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6180
Entity Detected:	Rab31, RAB31, member RAS oncogene family ( MGI:1914603)
Sequence:	sense strand is shown >T6180 GCCGAGGCCCAGAAATAGCCGCCTTGGCGGTTCCGCCCCGCGGCCGGCGAGAGTCCTGGGAGACAGCAGG ACGCAGGCTGGGCGCGGTGTGGCCCGGACGATGCTGCTGAGCCCCGGCCGGGCCCGACCCAGAGCACATG ATGGCGATACGGGAGCTCAAAGTGTGTCTTCTCGGGGACACGGGGGTTGGGAAATCCAGCATTGTGTGTC GTTTTGTCCAGGATCACTTTGACCACAACATCAGCCCCACTATTGGGGCATCTTTCATGACCAAAACCGT GCCTTGTGGAAATGAACTTCACAAATTCCTCATCTGGGACACCGCTGGCCAGGAGCGGTTCCACTCCCTG GCTCCTATGTACTACCGAGGATCTGCTGCAGCCGTCATCGTCTATGACATCACTAAGCAGGATTCATTTC ATACCTTGAAAAAATGGGTCAAGGAGCTGAAGGAGCACGGCCCAGAAAACATTGTGATGGCGATTGCTGG GAACAAGTGTGACCTCTCTGATATTAGGGAGGTGCCCCTGAAGGACGCTAAGGAGTACGCTGAATCCATA GGTGCCATCGTGGTGGAGACCAGCGCGAAGAATGCCATTAACATCGAGGAGCTCTTTCAAGGAATCAGCC GCCAGATCCCACCCTTGGGCCCTCAGGAAAATGGAAACAGCGGCGGGATCAAGCTTGGGAATCAGAGCTT GCAAGCCAGCCGTCGGTGCTGCTGAGCCAAGGGCATGGCATACAGAGTAAGAAGGAAGGAGCCAGAGCCC TCTTTGTTGGTGTCCAGTGCACACACAAGGACCCCACCCGTGGGGCTAACTACCTGCCGGAGAGTGAGCA GCTTTGTACCCTGGGACACCTGCTGCAGGATGCTGAACAGGGACGTTTCCTGCAAAGAAATGATAGAAAA CTCTGGAAAACTCACCACCACCGCAACATGGCCTTCAGTTTATGAAATGCACACAGCAAGGCTTGTACTT GCTAGGTAATAGATATAGTTGCCGTTTTTTTTTTTTTTTTTTGTTAAAGAGGTATTAGAAAAGTTTTAGA TTGGTCCACACACCTCAGAGTGCTGTTCTTTGCTAATTTCCTATGTCACCTAGGCAATCTCAATAATGTC TCCAGGGCCAGGGCCTTCCTTTCTGGCCAGTTCACCAAAAGGCAAAGGCTATAATAGGAGGTTAGCGCTT AGCTGGCTTTGGAAGTAAGTCTTTATTAGTTTTCTGAGGGAGATCAGAGATGCACAGCTGGACCACTGCT TAAACTGAAAAGTGAACTCCTGTAAGAGAGACAAAAGTTGCATTAGAAACCACCATCCCTATCTCCTTTC ATTTATCTGATGGACATTTTTCTAAGGTATGGTCATCTGTAACCCAACCAAATCAAACTAGAATGTTACA TATAATTTTGGAGTGGTGGGGGCTGATGAAAAGAGTTAAAATTAGTTGCTATATAAGCAGTGGTGGTTGG AAGATCTAAATGGGATTCGGGGTACCCCCAAGCATGCACCCCCCTATTGCAGTGAGTTTCTACTTTTGGG GTGATCACTTCACAGAGCATTAGGAGACAATGTCATGATGTCACATCTGGGGATGTTTGAGCCTAAATTG TAGGGAAGACTTCTGTTAATAGCAGCTAATCATAGGTTGTAGCAAACCAGGCAGAGACATGTTACCACCC TCAAATTCTGGACCCCTAAAGGTGTGAAATCAGAAGACATCCTTAAACTTTGCCAACTTTGGAGAGAAAG GGTGTCAAACAGTCAGTGCCCAGTTCATTGACGGTGCTTGTCCGCTCCAGGACGTGAGCCTCAACATAGT CTTCCTGCTTCTCCCAACTTGGGAGCTAATTGAGTCCTAGAATACAGGGGATAGCAAATACTTGTCCTGA TTCAAGAGAATCAAGACTCCTACATGTCCCATAGCAGGAGTCTCACTGTCAGCAAATGGCATCTGTATGC TGTGCCAGACTTCTCAAGACCATCAGTGCCTATCCCATGTCTCCAATATCAGCAGCTGACAGGATTCCCC ATGCTGGCAAAGACATTCGTCCACTCACTACAGGTCAAACAAACAGAGAACGATTGAGCTGAAGTTGAGG TGACCTCTCTCGAGTACCAAGCTTAGTTTTGAATCTTTCCCCATTTTTGAGGGGAACCTTTTGCCGGGAG CTTGTATTTACCTGGATGACGGTCTAGATTTCCAAGCCAAATTGGTACTGTTCCCAGGAGAGGATGCCCC AGCTTTGGATTGGGTTTGCAGTGGTTGAGTTTTGCATGAACTGTCTGGGCTTCACCTTGAGAGTGAGCTG CCCTGGGTTGGATCAGAGAAGCATTGTGAGGGTACGACCCCTTGCTTTTCCCCAGGCTGCATGCTGTTGA TGTCACAGCCATGCAGGGGGTAAGTGTGCACACGTGCCACCAGCCTGAGCAGAGAGAGACGTGGCTTGGG CACACAACTACTCACACCACTTTGAGCCTTCTCTGCAGGTAGGTGCTAAGGCCTTTGATGCTTCTCCTCT TGGCTTGGCATTCCTTTTGGGATGTTTTTAACCACTGACCCTCGACAAAGACCTAGACACGACAATTGCT CCAGACATAGGATGTAGTGTCCTTATGGCTACTCCCCAAATTCTGTTCCCAACCCCCATTTTCTTATCTC TCACAGGTGAGCGGGTTTGTATATGTGTAAATATGGTTTTTATGTCAACTGCACCTACATGTAATCCATT TTAGAGTGGCTGTGTTTGGCTCGTGACCTTGTGCCCATAAGTGAGGCACAGAATGTCAAAGGGATTCTTT CTCAGGAGACGTCCCCAAAGGACAGTGGCAGGGCTGTGCAAATGTTTTATACAGGTACAGAATTGACTCA CCATTTTTTTTTCATTAAAAGTGGTATATTAGGGTACTGGATATGACGTCATCTTGCAGACTAACCTGCA TCCTAGCCAACAGCAAGGGCTGTGCCCCAGTAGGAATCCCGTTCTTTCCTGAGGTTAGGTTTGGAGGATC AACATGTTTCCTCCGCCATAATGGACTCTGGCTTGTGTAGGCTTCTTGCTGCTCCGTAGCATTCCGGGCG AGGCGTAAGGAGGCAGTGGTGGTCAGCCAGGGATTCTGGCTGTGCTCATGTGATCACGGCTTTCACCATT GTCGTGACACCCAGTGACTCAGCAGTCATACCGTCACACAGACTGTCGCCCTCTCCGGTGTTTTTGTAAA GGGTTTCCACTTGGGCTGGACCCAGGTTTGGTACCTAGAAGATCCTTCTGCAGACACCGAGGACCTTCGC ATCTTGTACTCTGTTCTCTGATAACGAGTTTCGGGTTGGCGCTCCTTTGTATCAATTGCTTTTGATTTTA ACCCATTGGTATGATTCCTACTAATAAATATTTTAAGTTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3497938 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3497938 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10633 same embryo
	EMAGE:10636 same embryo
	EMAGE:10635 same embryo
	EurExpress:euxassay_003248 same experiment
	MGI:4827575 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source