EMAGE mouse gene expression spatial database

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EMAGE:10657

Gramd1a GRAM domain containing 1A ( MGI:105490)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000938. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000938_01	euxassay_000938_02	euxassay_000938_03	euxassay_000938_04

euxassay_000938_05	euxassay_000938_06	euxassay_000938_07	euxassay_000938_08	euxassay_000938_09

euxassay_000938_10	euxassay_000938_11	euxassay_000938_12	euxassay_000938_13	euxassay_000938_14

euxassay_000938_15	euxassay_000938_16	euxassay_000938_17	euxassay_000938_18	euxassay_000938_19

euxassay_000938_20	euxassay_000938_21	euxassay_000938_22	euxassay_000938_23	euxassay_000938_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10657_wholemount_strong.wlz

10657_wholemount_moderate.wlz

10657_wholemount_weak.wlz

10657_wholemount_possible.wlz

10657_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10657_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

weak

regional

weak expression: see section 06 07 08 21 22

liver lobe

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

skeleton

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 10 11 12 13 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T430
Entity Detected:	Gramd1a, GRAM domain containing 1A ( MGI:105490)
Sequence:	sense strand is shown >T430 CTCTACCTCTCTGAGAACTGGATCTGTTTCTACAGCAACATCTTCCGCTGGGAGACCACAATTTCCATCC AGTTGAAGGAAGTGACCTGTCTGAAGAAGGAGAAGACGGCTAAGCTCATCCCCAATGCCATTCAGATCTG TACTGAGAGTGAGAAGCATTTCTTCACTTCCTTCGGGGCCCGAGACCGCTGCTTCCTCCTCATCTTCCGT CTCTGGCAGAACGCGCTGCTTGAAAAGACACTGAGTCCTCGAGAGCTTTGGCACCTGGTCCATCAGTGCT ATGGCTCGGAGCTGGGCCTCACCAGTGAAGACGAAGACTATGTCTGCCCCTTGCAACTGAATGGTCTTGG GAGTCCCAAAGAGGTGGGAGACGTGATCGCACTCAGCGACATCAGTCCCTCGGGGGCGGCTGACCACAGC CAGGAGCCAAGCCCTGTGGGTTCAAGACGTGGCCGGGTCACCCCAAACCTCTCTCGAGCC
Notes:	The probe template was PCR amplified from IMAGE:3164292 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3164292 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10658 same embryo
	EMAGE:10656 same embryo
	EMAGE:10659 same embryo
	EMAGE:10655 same embryo
	EMAGE:10654 same embryo
	EurExpress:euxassay_000938 same experiment
	MGI:4825210 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source