EMAGE mouse gene expression spatial database

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EMAGE:10694

Vps33a vacuolar protein sorting 33A (yeast) ( MGI:1924823)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001227. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001227_01	euxassay_001227_02	euxassay_001227_03	euxassay_001227_04

euxassay_001227_05	euxassay_001227_06	euxassay_001227_07	euxassay_001227_08	euxassay_001227_09

euxassay_001227_10	euxassay_001227_11	euxassay_001227_12	euxassay_001227_13	euxassay_001227_14

euxassay_001227_15	euxassay_001227_16	euxassay_001227_17	euxassay_001227_18	euxassay_001227_19

euxassay_001227_20	euxassay_001227_21	euxassay_001227_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10694_wholemount_strong.wlz

10694_wholemount_moderate.wlz

10694_wholemount_weak.wlz

10694_wholemount_possible.wlz

10694_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10694_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05 06 18 19 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 16 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 07 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 16 17 18

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 16 weak expression: see section 10

cervical ganglion

moderate

regional

moderate expression: see section 08 16

thoracic ganglion

weak

regional

weak expression: see section 11 12 13 15

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4214
Entity Detected:	Vps33a, vacuolar protein sorting 33A (yeast) ( MGI:1924823)
Sequence:	sense strand is shown >T4214 GGGGAAATGCGGGTGCCCGGGCAAGATGGCGGCGCACCTGTCGTACGGGCGAGTGAACCTGAACGTGCTG CGCGAGGCGGTGCGTCGGGAGCTGCGCGAGTTCCTGGACAAGTGCGCTGGGAGCAAGGCAATAGTGTGGG ATGAGTACTTAACAGGACCATTTGGACTGATCGCACAGTACTCACTACTAAAGGAACATGAAGTGGAAAA AATGTTCACACTTAAAGGAAGTCGTCTGCCCGCAGCTGATGTCAAGAACATCATTTTTTTGGTCAGACCC AGGCTAGAGCTGATGGACATAATTGCTGAGAATGTACTCAGTGAGGACAGGCGTGGCCCTACCAGGGATT TCCATATTTTGTTTGTGCCTCGCCGGAGCCTACTGTGTGAGCAGCGGCTGAAGGACCTGGGGGTTTTGGG ATCCTTTATTCATCGAGAAGAATACAGCTTGGATCTCATTCCATTTGATGGGGACCTCTTATCCATGGAG TCAGAGGGCGCTTTCAAAGAGTGCTACCTGGAAGGCGACCAGACCAGCCTGTACCACGCAGCCAAGGGGC TGATGACCCTGCAGGCGCTGTATGGAACTATCCCCCAGATCTTTGGGAAAGGAGAGTGTGCTCGGCAAGT GGCCAATATGATGGTCAGGATGAAGAGGGAGTTCACAGGAAGCCAGAACTCAGTGTTCCCCGTGTTTGAT AACCTCTTGCTGCTTGATCGCAATGTGGATCTCCTGACGCCACTCGCCTCCCAGCTCACGTATGAAGGCC TCATCGATGAGATTTACGGCATTCAGAACAGTTATGTGAAGTTGCCTCCAGAGAAGTTTGCACCCAAGAA GCAGGGTGGCGGCAGTGGTGGGAAGGACCTTCCCACGGAGGCCAAGAAGCTTCAGCTCAACTCCGCAGAG GAGCTCTACGCCGAGATCCGAGACAAGAACTTCAACGCTGTGGGCTCTGTGCTCAGCAAGAAGGCGAAGA TCATCTCTGCAGCGTTCGAGGAGCGACACAACGCTAAGACAGTCGGGGAGATCAAGCAGTTTGCGTCCCA GCTACCCCACATGCAGGCAGCCAGGGGATCGCTCGCCAACCACACCTCAATTGCAGAGTTAATCAAAGAC GTCACCACTTCTGAAGACTTCTTTGATAAGTTGACAGTGGAGCAGGAGTTCATGTCCGGGATAGACACTG ATAAGGTCAACAGTTACATCGAGGACTGCATTGCCCAAAAGCATCCACTCATCAAGGTGCTGAGACTGGT TTGCCTCCAGTCTGTGTGCAACAGCGGGCTCAAGCAGAAGGTGCTGGACTATTACAGAAGGGAGATCCTC CAGACCTACGGCTATGAGCACATACTGACCTTGAACAACCTGGAGAAGGCCGGCCTGCTCAAGGCTCAGA CAGGGGGCAGAAACAATTACCCAACAATCCGGAAAACACTGCGCCTCTGGATGGACGATGTCAATGAACA AAACCCCACAGACATATCCTATGTGTACAGCGGTTATGCTCCACTCAGCGTGCGACTGGCTCAGCTCCTT TCCCGGCCAGGCTGGCGGAGCATCGAGGAGGTTCTCCGCATTCTCCCAGGGCCCCACTTTGAAGAAAGAC AGCCACTGCCCACCGGGCTGCAGAAGAAACGCCAGCCAGGAGAAAACAGAGTCACACTGGTATTTTTCCT CGGGGGCGTCACCTTTGCGGAAATAGCCGCCCTGCGATTTTTGTCCCAGCTGGAAGATGGAGGCACCGAG TATGTCATTGCGACCACTAAACTGATGAATGGAAACAGCTGGATAGAAGCTCTCATGGAAAAGCCTTTCT AGAGTATCGGAGGAGACGTGACAGGTCACCACACAATGAGCTGCCTCTGTGAACGTGTTGCCAAAGGATG TAAGGCCAGGAACAGGAGGACGGAAGCAGTGGGCGCATGTTCCTTCCTGCTCCTCTCTATGCTCTTAATC CTAAAGAAGGGACAAGCAACCTGGATGAAGGGATGATGAACTGGGCATAAGAGACAGAGGCAGGAGGATT GCTGCAAGCTTGAGGACAACCTGGGCTCCATAATGAGTTTCAGGCCAAAGCTCTCCCTTCCAGAAATAAA GGAGATCCTGTTTTGGAATTTTTAAGTCTCAAGTCTTCATTTTCTGGAAATGGCTAAGACTATGGGTCTG ATAGCCCTGGCAACCACATGACTGCACAGACATTAAAAGCAGAGCTGAGGGGACGAATTCAAAAAGAGAA TCAGAATTCTGACCACTGATCCACAGTGGCATATTCTAGAAGATGTCCCATGCCCAGGACTGTGGTTTTC TGTTGAATTGGAAGCCAGCATTGTCCAGTGTGTGCACTTTCCTGCAAAGCACATTTGTGTCTCCAGTCTC TACCGAGTTATTCTTAAGGCGAGCTTTGTTTCCTTCTGAACAACTAAGAGGTTCGAAGGGGCTTCTTGGC ATAATGACATTTCTGCTCTCTGCGTTAGCCATGACTGTGCAGGTCTCTGCCCAGAAGGGACTGATCAAGA GTGAGCTGAGCGCCAGGCAGCCTCTTCCAGCTAACCTTCCAAAGGCACTTCTGAGCGTGCATGTTGGTAA CTCCTGGACTCACCAGGGTAGGCGCTCCTGCCGGGACTTCTGTTTTCCTCCATGTCCTCCATGGGCAGTT CTTCTGTTCTTTCAATATCATGAAGCTCTGTCTCCGTGGTAACTGCCACACACAGCTCTCTAGCCCTCTA GCTGTACTCAACTCTGCACACCCCCTTTCCCTGAAAATACTCAGGTGGTCAGTGCACTTCGGTCAGTGTC AGTCACAAGAGACAAGCAGCACCAGTGCTCAGGGAGTGGAGCACGGCCGGAAGCAGAGCAGGCATGGCCT CCTGATCATCTCAGCATCTGAAGGAGCTAAGAGCACCAAGTGGGGGACCACGTTGATGGCTTTCCTGTCA TCACTCAAGACTCCAGAAAAGCTAGCCTGTCTCAGCCGGTCCTGGTCCTTGCCCAGGTGCAGTTTCTATG GTTAATATTTTCTTCTTGTTTTTCTCATGTAGAATCAGGAGCAATACACTGCACATTATAAGTAACCAGA GTTGGGTATTTAAAAGAAAAAAACCTAGGAATGTAGGATGGCAAAGATGATATTTGCTTCTCGTGGCGTT AATTCAGTATTGGGAGAGTCAGGCGGTGTGGGGGGCATTCCCAAATGGGAAATCATTCTTAACTTAGAAG ACTCTTTTTCAGATTCTTGTTACTCCAGGGAGACTAGTTTTACCTTTGAGCCCCAGCAATGGGTAGTTTC AGCTGCCAGGGGCTGCAATCCACCTTGTCCTCAGTGTTTTCCACTCTAGGGATTCAGGACAAGTCTGTCC ACGTCTCCTTGGCGTGGGTGTTTGTCCAGTGAACAGACACTCAGTTCTAATCTCATGTGTCTGCATTTGT TTCTGAGTCTATATTTGCTTTCTCAGAGCATAGCAAGGTCAACTCCTAAAAATGTACCCAGCAGAAGACC TTTTTGAAAAACCACACACTTGCTTCCTCTGTATTCACACACAAAGTGGTGGTATCATACATTATGCCCT GTGATGTAAGTCCCCTTAGTGCTCAAAGCTGCCTTTGTCTCTTAAGAGCGTGCACTGAGAAAGGCTGTGG GAACGGAAGGCTTATTCTCAGACAGGGTGGTTTGTTTACCCCATGTCTGGACTTGTCCTGAGTCTGAGGA CCACTCTGCTGCACTGCCCGTCCTGATTAAAGACGTCTCTGTAGATGCTAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:4501207 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4501207 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10693 same embryo
	EMAGE:10695 same embryo
	EurExpress:euxassay_001227 same experiment
	MGI:4829170 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source