EMAGE mouse gene expression spatial database

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EMAGE:10695

Ak3 adenylate kinase 3 ( MGI:1860835)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003106. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003106_01	euxassay_003106_02	euxassay_003106_03	euxassay_003106_04

euxassay_003106_05	euxassay_003106_06	euxassay_003106_07	euxassay_003106_08	euxassay_003106_09

euxassay_003106_10	euxassay_003106_11	euxassay_003106_12	euxassay_003106_13	euxassay_003106_14

euxassay_003106_15	euxassay_003106_16	euxassay_003106_17	euxassay_003106_18	euxassay_003106_19

euxassay_003106_20	euxassay_003106_21	euxassay_003106_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10695_wholemount_strong.wlz

10695_wholemount_moderate.wlz

10695_wholemount_weak.wlz

10695_wholemount_possible.wlz

10695_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10695_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 06 07 16 17 18 moderate expression: see section 08 09 15

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 10 11 12

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 10 11 12

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 09 10 14 15 16

telencephalon ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 14 15 16 17 18 19 20 21 weak expression: see section 11 12 13

midbrain ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1114
Entity Detected:	Ak3, adenylate kinase 3 ( MGI:1860835)
Sequence:	sense strand is shown >T1114 TCCTCNAGNCTGTTGGCCTACTGGACAGACCAGCGTCTGCGCAGCTCGGACCACCGCCAGCCACCATGGG GGCGTCGGGGCGGCTGCTGCGCGCCGTGATCATGGGGGCCCCGGGCTCCGGCAAGGGCACCGTGTCGTCA CGCATCACCAAACACTTCGAGCTGAAGCACCTCTCCAGCGGGGACCTGCTCCGCCAGAACATGCTGCAGG GCACAGAAATCGGTGTGTTGGCCAAGACTTTCATTGACCAAGGAAAGCTGATCCCAGATGACGTCATGAC TCGGCTGGCCCTTCACGAGCTCAAAACCCTTACCCAGTGTAGCTGGCTGTTGGATGGATTTCCAAGGACA CTTCCACAGGCAGAAGCCCTGGATAAAGTTTATCAGATAGACACAGTGATAAATCTCAACGTGCCCTTTG AGGTCATTAAACAGCGCCTTACTGCTCGCTGGATACATCCTGCCAGTGGTCGAGTATACAACATTGAATT CAACCCTCCCAAGACTGTGGGCATTGATGACCTGACCGGAGAACCTCTGATTCAGCGCGAGGATGACAAA CCAG
Notes:	The probe template was PCR amplified from IMAGE:2135343 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2135343 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10694 same embryo
	EMAGE:10693 same embryo
	EurExpress:euxassay_003106 same experiment
	MGI:4823058 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source