EMAGE mouse gene expression spatial database

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EMAGE:10727

2900011O08Rik RIKEN cDNA 2900011O08 gene ( MGI:1914504)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000995. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000995_01	euxassay_000995_02	euxassay_000995_03	euxassay_000995_04

euxassay_000995_05	euxassay_000995_06	euxassay_000995_07	euxassay_000995_08	euxassay_000995_09

euxassay_000995_10	euxassay_000995_11	euxassay_000995_12	euxassay_000995_13	euxassay_000995_14

euxassay_000995_15	euxassay_000995_16	euxassay_000995_17	euxassay_000995_18	euxassay_000995_19

euxassay_000995_20	euxassay_000995_21	euxassay_000995_22	euxassay_000995_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10727_wholemount_strong.wlz

10727_wholemount_moderate.wlz

10727_wholemount_weak.wlz

10727_wholemount_possible.wlz

10727_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10727_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

telencephalon marginal layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 10 11 12 13 15 16 17 18

medulla oblongata alar plate mantle layer

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

medulla oblongata basal plate mantle layer

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

metencephalon rest of alar plate mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22

pons mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain mantle layer

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain marginal layer

moderate

homogeneous

moderate expression: see section 13 14 15

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 weak expression: see section 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19 weak expression: see section 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 18 19 weak expression: see section 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 09 19 not examined expression: see section 10 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 09 weak expression: see section 18 19 20

spinal cord mantle layer

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15 16 17 18

cervico-thoracic ganglion

weak

regional

weak expression: see section 11 12 17 18

cervical ganglion

weak

regional

weak expression: see section 10 18

thoracic ganglion

weak

regional

weak expression: see section 13 14 15 17

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 13 17 18 19 20

cornea stroma

strong

regional

strong expression: see section 01 02 03 weak expression: see section 04 23

genital tubercle of male

moderate

regional

moderate expression: see section 12

tail dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 13 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4322
Entity Detected:	2900011O08Rik, RIKEN cDNA 2900011O08 gene ( MGI:1914504)
Sequence:	sense strand is shown >T4322 TGAAGAGATGGAGCAGCTTCCTCAGCAACGCCGGGCCCATTAAAACACTGCCGATCTGAGCTGCCTTCCT CCCCGCAGCCTTCAGCAGGGATGGAGTGAACCAGACCCCTGGACCTGCCCGCGGGGAACAGCAATGGAGT TAAAGCAATCTCTGTCTGTTCATCTGGAAGCCGAGAAGCCTCTCAGGCGTTATGGGGCGGTGGAGGAGAC AGCATGGAAAGCGGAGGGACTGGGAAGCCAGCTAGACATCATCTCCATGGCTGAGACCAGCATGATGCCA GAGGAGATCGAGCTGGAGATGGCGAAAATCCAGCGTCTCCGTGAAGTCCTGGTCCGTCGAGAGTCAGAAC TCAGGTTCATGATGGATGACATTCAGCTCTGCAAGGACATCATGGACCTGAAGCAGGAGCTGCAGAACTT GGTCGCCATCCCAGAAAAAGAAAAAACCAAGCTGCAGAAGCAGAGAGAGGATGAGCTCATCCAGAAGATC CACAGACTGGTACAGAAGAGAGACTTCCTGGTGGATGATGCCGAGGTGGAACGCTTAAGGGAACAGGAAG AAGACAAGGAAATGGCTGATTTCCTGAGAATCAAGTTAAAACCTCTAGACAAAGTAACCAAGACTTCAGC CAGCTCCCGGGCAGAGAAGAAGGCAGAGCCCCCACCTAGCAAGCCAACAGTGGCCAAGACAGGGCTGGCA CTCATCAAGGATTGCTGTGGGACCACCCAGTGCAACATCATGTAGCCCCTATGTGGGTGCCCCAGAGCTA TGGGGACCCTTCCTCCCACCCTCTTGTCCTCATAGCCCCCATTTCACAGGGGTACAAGAGCTTTCCAAGG CAGAAGGGATTGACAGCAAGCCCATGACTGCTATCAGGGGCTATGGGTATGGCAGGTGGGCCCAGTCTGT ACAGCGTGTAGCAATAGAGAGCCCCTCACTGTCGCATGACCCTCCCCAGAGCATGCCCAACCCAGGAGTC TGTCTTATCATTTATCTAACCACCAAGAAAGCCCCTCCCTTAAAACAATAGACCTTCACTTTTCTCTAGG TGTATCTAACCTACCATGCAAACGAACTGGGCAAGGGCATAGCCCCAGGTGTGTATAGCTGTGAATTTCA ATAATCTAGCACAGTGTTGGTCCCATTCCCCACCTACCCTCCCAGCTTTGCTGTCAGATACAAGTCCCGT TCCCTGTTTGCCCTCTCCCAGGGACTGTAGCTTGGAGGGTTCCACACAGCCCACACACCTCTTAGGCTCC ATTGTAATCACTTCCCTAAAGACACTGGCCTGTCTATGAAAGCTGAGTGTGTGTGGTGTTGGGTTTACAC CTCCTGCTCCATCCCAGCCTGGTGTGTATCTATCTGCCAGACTCCAGGCTCTTCCTTGATTTGCCATGCC CAGCCCACAGTGGAACAGAGTAGGGAACTGTGACCAAAAGCAGATCCAGTGCTTGGACCAGGTAGCAGAG AACTGCAGGGACCAATATCAAGACCAGAGTGAATGAGAAGGGGACCCAGAAAAGATGCCAACCCACATCC TCTTCACCCAGGGCTGCTGCAAATGAGTACATACAAGTGGGAAGGTCACACTGCTGGCCCAACACACACT GACAGGAAGCCTGACCCAACTATGTCCCATTGTCTGGTGGCGTGTTTGAACCAGCCCCACAATTCGTCTT GTGTAACTTGATGTACATTTTAAATGGCCAGCCCAGCACAGCCTGTGTGAACTCTGTGCGTGTATGTGTG TTATGACTGGCCTAAGTACTTAGCACCAGTCAAGATTTGCTGATGTGCAAACTATCCATCAGAGAAAAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4511454 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4511454 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10726 same embryo
	EurExpress:euxassay_000995 same experiment
	MGI:4822693 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source