EMAGE mouse gene expression spatial database

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EMAGE:10756

Dact2 dapper homolog 2, antagonist of beta-catenin (xenopus) ( MGI:1920347)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001023. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001023_01	euxassay_001023_02	euxassay_001023_03	euxassay_001023_04

euxassay_001023_05	euxassay_001023_06	euxassay_001023_07	euxassay_001023_08	euxassay_001023_09

euxassay_001023_10	euxassay_001023_11	euxassay_001023_12	euxassay_001023_13	euxassay_001023_14

euxassay_001023_15	euxassay_001023_16	euxassay_001023_17	euxassay_001023_18	euxassay_001023_19

euxassay_001023_20	euxassay_001023_21	euxassay_001023_22	euxassay_001023_23	euxassay_001023_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10756_wholemount_strong.wlz

10756_wholemount_moderate.wlz

10756_wholemount_weak.wlz

10756_wholemount_possible.wlz

10756_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10756_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 12 13 14 15 16 17

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 11 18 19 20 21

foregut-midgut junction

moderate

regional

moderate expression: see section 12 13 14 15 16

esophagus

weak

regional

weak expression: see section 13 14 15

stomach

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10

hindgut

moderate

regional

moderate expression: see section 12 13 14 15 16

midgut

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

oral epithelium

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

kidney calyx

moderate

homogeneous

moderate expression: see section 08 09 12 20 21 22

kidney pelvis

moderate

homogeneous

moderate expression: see section 10 11 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T341
Entity Detected:	Dact2, dapper homolog 2, antagonist of beta-catenin (xenopus) ( MGI:1920347)
Sequence:	sense strand is shown >T341 GCGGGGAGCCCTGGGCCTGCACCCTGCGCCAGGGCCCCGCGGCCAGGAGCTGCGTCTGGAGGCGGCGCTG ACCGCGCTACGGGAGCAGCTGTCACGGCTAAGGAGACAGGATGCTGGCCTGAAGACACACTTGGACCAGC TGGATCAGCAGATAAGTGAACTACAGCTGGATGTGAGCAGGTCTTCTTGCGAGGCCTTGGATAGTGACAG CAGACCCAGCTCAGGTTTCTATGAGCTGAGCGATGCTGGTTCCTGTTCTCTGTCCACCTCCTGCGCATCT GTTTGCAGCGACCGTCTATCCCCCTCCCTGGGTAGCTGGCTGCCTGTGTTCCAGCCCTCCAAGTCCAGGT CTGGCATCGGGGACT
Notes:	The probe template was PCR amplified from IMAGE:3154693 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3154693 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10758 same embryo
	EMAGE:10760 same embryo
	EMAGE:10757 same embryo
	EMAGE:10755 same embryo
	EMAGE:10759 same embryo
	EurExpress:euxassay_001023 same experiment
	MGI:4824223 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source