EMAGE mouse gene expression spatial database

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EMAGE:10759

Cdkn2a cyclin-dependent kinase inhibitor 2A ( MGI:104738)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002902. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002902_01	euxassay_002902_02	euxassay_002902_03	euxassay_002902_04

euxassay_002902_05	euxassay_002902_06	euxassay_002902_07	euxassay_002902_08	euxassay_002902_09

euxassay_002902_10	euxassay_002902_11	euxassay_002902_12	euxassay_002902_13	euxassay_002902_14

euxassay_002902_15	euxassay_002902_16	euxassay_002902_17	euxassay_002902_18	euxassay_002902_19

euxassay_002902_20	euxassay_002902_21	euxassay_002902_22	euxassay_002902_23	euxassay_002902_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10759_wholemount_strong.wlz

10759_wholemount_moderate.wlz

10759_wholemount_weak.wlz

10759_wholemount_possible.wlz

10759_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10759_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 05 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 09 20 21

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 19 20 21 22 23 24 weak expression: see section 10

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 08 09 20 21

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T305
Entity Detected:	Cdkn2a, cyclin-dependent kinase inhibitor 2A ( MGI:104738)
Sequence:	sense strand is shown >T305 TGCTGGAAGCCGGGGTTTCGCCCAACGCCCCGAACTCTTTCGGTCGTACCCCGATTCAGGTGATGATGAT GGGCAACGTTCACGTAGCAGCTCTTCTGCTCAACTACGGTGCAGATTCGAACTGCGAGGACCCCACTACC TTCTCCCGCCCGGTGCACGACGCAGCGCGGGAAGGCTTCCTGGACACGCTGGTGGTGCTGCACGGGTCAG GGGCTCGGCTGGATGTGCGCGATGCCTGGGGTCGCCTGCCGCTCGACTTGGCCCAAGAGCGGGGACATCA AGACATCGTGCGATATTTGCGTTCCGCTGGGTGCTCTTTGTGTTCCGCTGGGTGGTCTTTGTGTACCGCT GGGAACGTCGCCCAGACCGACGGGCATAGCTTCAGCTCAAGCACGCCCAGGGCCCTGGAACTTCGCGGCC AATCCCAAGAGCAGAGCTAAATCCGGCCTCAGCCCGCCTTTTTCTTCTTAGCTTCACTTCTAGCGATGCT AGCGTGTCTAGCATGTGGCTTTAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2812276 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2812276 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10756 same embryo
	EMAGE:10758 same embryo
	EMAGE:10760 same embryo
	EMAGE:10757 same embryo
	EMAGE:10755 same embryo
	EurExpress:euxassay_002902 same experiment
	MGI:4823787 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source