EMAGE mouse gene expression spatial database

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EMAGE:10767

Zscan12 zinc finger and SCAN domain containing 12 ( MGI:1099444)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001284. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001284_01	euxassay_001284_02	euxassay_001284_03	euxassay_001284_04

euxassay_001284_05	euxassay_001284_06	euxassay_001284_07	euxassay_001284_08	euxassay_001284_09

euxassay_001284_10	euxassay_001284_11	euxassay_001284_12	euxassay_001284_13	euxassay_001284_14

euxassay_001284_15	euxassay_001284_16	euxassay_001284_17	euxassay_001284_18	euxassay_001284_19

euxassay_001284_20	euxassay_001284_21	euxassay_001284_22	euxassay_001284_23	euxassay_001284_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10767_wholemount_strong.wlz

10767_wholemount_moderate.wlz

10767_wholemount_weak.wlz

10767_wholemount_possible.wlz

10767_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10767_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5383
Entity Detected:	Zscan12, zinc finger and SCAN domain containing 12 ( MGI:1099444)
Sequence:	sense strand is shown >T5383 GGAGCGACGCGGTCCACTGGGTAAGAAGATTTACCAAGGAGTTATTACTTGAAAGATTGATACATCTTTT ATCTAGCTACGATGACATCTACTAGTGATACCAAGGTCTGTAAGAACCAGGGTGGACTTTTGGAAATAAA AATGGAGGAAGAATGTAAGTATACCACCAGACAAGATAGGAACCTACAAAAGAACACTTATAACAGAGAC GTCTTCCGAAAATACTTCAGACAGTTTTGCTACCAGGAAACTTCTGGACCCCGGGAGGCTCTGAGCCGGC TGCGCGAGCTCTGCAGACAGTGGCTGCGGCCGGACTTGAACAGCAAGGAGCAGATCCTGGAGCTGCTGGT GCTGGAGCAGTTCCTGACCATCCTGCCAGGGGAGCTGCAGGCCTGGGTGCAGGAGCAGAATCCGGAGAGT GTGGAGGAGGTGGTGACTGTGCTGGAGGATTTAGAGAGAGAGCTTGATGAACTAGGATACCGGGCCTCAG TCCAAACTGAAGAACAGGTAACGTTTCAGGAGGTGAAGCCTTTGGCAACAGAGCAGAAACCCAGCGTGTC CCTTCAGTTTGTCAAAGCCAAGCCTGGGTGTGAACTTGCAGGCCGTGAAGCCCAGGAGGAGCAAGTTTCA GGTGTTGAGACTGGCAATGAGCCCAGGAATGTCACTCTAAAGCAAGGCCTCTGGGAAGGGACGGAAGCAG AACAGAATCCTGCTAGCAGATTAGCAAAGGATGCACTTGAGTGTGAAGAAGCTCACAACCCTGGAGAAGA GTCTTCTGGTATTTCCCATGAAGACAGCCAGCCTCTGCGTAATGAAAATGGTGTAAATTCTCCTGCGAAC TCAGAGTACGCTAAACACCAGAGCATCTGTCCAGGGAGAAAAGTGCATGGGTGTGATGAGTGTGGAAAGA GTTTTACTCAGCACTCGCGCCTCATAGAGCACAAGAGAGTCCACACTGGAGACAGGCCCTACAAATGTGA AGTATGTGGGAAAACTTTCCGATGGAGGACTGTTCTTATTCGACACAAGGTGGTCCACACTGGAGAGAAA CCGTATAAATGTAATGAATGTGGCAGGGCTTTTGGCCAGTGGTCAGCACTTAACCAACATCAGAGACTTC ACTCGGGAGAAAAGCACTACCACTGTAACGAATGTGGCAAAGCCTTTTGCCAGAAAGCAGGCCTCTTTCA CCATCTCAAGAGCCATAGAAGAAACAGACCTTATCAATGTCTTCAGTGTAATAAAAGTTTTAATCGCCGT TCTACACTTTCTCAGCATCAAGGAGTTCACACTGGAGCAAAACCCTACGAATGCAACGATTGTGGGAAAG CTTTTGTTTATAACTCATCTCTTGCTACCCATCAGGAAACCCATCACAAGGAGAAACCCTTCACTCAAAG TGGTCCTATTCAGCAGCAGAGGAACCACACCAAGGAGAAGCCCTACAAATGCAGTGTATGTGGAAAAGCA TTTATTCAAAAAATAAGTCTTATAGAACACGAACAAATTCATACCGGAGAGAGACCGTATAAATGTGCTG AGGGCGGGAAGGCCTTTATTCAGATGTCAGAACTCACAGAACATTAGGGAACCCACACTGGGAAGGCCCT ACAAGTGTGATGAATGTGGCAAGACCTTCAGACAGGAGTCAGAGCTTGCTGAGCACTAGAGAATTCATTA TAGAAGTGGTCCCTAAAAGTGTAATGGGCGTGGGGAATTGTTCACGTAGAACTCAGCTCTTACTCATCAT CAGACTAGTCACAAAAGAGCAAGTACTCTGTGTGATGACTACAGGGAAGCTACCTCACGGTGTCGGCTGA TGAAGAGTATTGTATTTGTAATGCTTTGTGGTGACATTTCAGCCTTTGCTGTTACGAGACCATAAATATT TGATGAGCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5364568 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5364568 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10766 same embryo
	EMAGE:10769 same embryo
	EMAGE:10765 same embryo
	EMAGE:10768 same embryo
	EurExpress:euxassay_001284 same experiment
	MGI:4829368 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source