EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10770

Bbs2 Bardet-Biedl syndrome 2 (human) ( MGI:2135267)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003014. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003014_01	euxassay_003014_02	euxassay_003014_03	euxassay_003014_04

euxassay_003014_05	euxassay_003014_06	euxassay_003014_07	euxassay_003014_08	euxassay_003014_09

euxassay_003014_10	euxassay_003014_11	euxassay_003014_12	euxassay_003014_13	euxassay_003014_14

euxassay_003014_15	euxassay_003014_16	euxassay_003014_17	euxassay_003014_18	euxassay_003014_19

euxassay_003014_20	euxassay_003014_21	euxassay_003014_22	euxassay_003014_23	euxassay_003014_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10770_wholemount_strong.wlz

10770_wholemount_moderate.wlz

10770_wholemount_weak.wlz

10770_wholemount_possible.wlz

10770_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10770_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4855
Entity Detected:	Bbs2, Bardet-Biedl syndrome 2 (human) ( MGI:2135267)
Sequence:	sense strand is shown >T4855 CGCAGTCCTGCGTGTATTTACCTCCTACTCCTCATCCGCTGCTAGGCAACCGGCCGGCCAAGCTGCTTGC CTTGCGCCTGCGTCGGGCATCTGTCTTCCGCCCCTAGCTGGCGCCATCATGCTGCTGCCTGTGTTCACTC TGAAACTGCGCCACAAAATCAGCCCCCGCATGGTGGCCATAGGGCGCTACGACGGGACTCACCCGTGCTT GGCTGCCGCCACCCAAGCGGGCAAGGTTTTCATTCATAACCCTCACACGCGGAGCCAGCATTTCAGTGCT TCCAGAGTGTTCCAGAGCCCTCTGGAGTCTGATGTCTCTCTGCTCAACATTAACCAGACGGTCAGCTGCC TGGGTTCGGGAGTCTTGAACCCTGAGCTTGGCTATGATACTCTTTTAGTGGGGACACAGACCAGTCTTTT GGCTTACGACATCTACAATAATTCAGATTTGTTCTACAGAGAGGTCTCAGATGGGGCAAATGCCATTGTG CTGGGGACCCTGGGAGACATTGCCCCACCTCTTGCAATCATCGGTGGAAACTGTGCTCTGCAGGGTTTCG ATCACGAAGGAAATGATCTCTTTTGGACGGTTACTGGAGACAATGTGCATTCCTTGGCTCTGTGTGACTT TGACGGTGATGGGAAGACAGAGCTTCTTGTTGGATCTGAAGATTTTGACATTCGAGTTTTTAAAGAAGAT GAGATTGTGGCAGAAATGACAGAGACAGAGATAGTCACCTCTCTGTGTCCTATGTATGGCAGTCGGTTTG GTTACGCCCTTTCCAATGGCACAGTTGGAGTTTATGACAAAACAGCCCGGTACTGGAGGATTAAATCCAA AAACCATGCCATGAGCATCCATGCTTTTGACATTAATTCTGATGGAGTGTGTGAACTGATAACCGGGTGG TCTAATGGGAAGGTTGATGCTCGCAGTGACCGGACTGGAGAGGTCATATTTAAAGACAACTTCTCTTCTG CTGTTGCTGGTGTGGTAGAGGGAGATTACCGGATGGATGGCCACGTACAGTTAATCTGCTGCTCAGTGGA TGGGGAAATCCGAGGCTACCTGCCAGGCACAGCTGAGATGAAGGGGAACCTCCTGGACACAAGTGTGGAG CAGGACCTGATCCGAGAGCTGAGCCAGAAAAAGCAGAACCTGTTGCTAGAACTTCGCAACTATGAGGAGA GCACCAAGGCAGAACTGAGCAGTCCCCTGAACGAGGCCGATGGGCAGAAAGGCATAATCCCAGCCAATAC CAGGCTCCACACAGCGCTCTCAGTCAATATGGGGAATGACTTACAAGATGCGCATGCGGAATTAGGCATT TCCACTTCCAATGATACTATCATCCGAGCAGTACTAATTTTTGCAGAGGGAATTTTTGTGGGTGAAAGCC ATGTGGTCCACCCCAGCATTCACAACCTTTCCAGCTCCCTCCGGGTACCAATTACACCTCCCAAAGATGT CCCTGTGGATCTGCACCTGAAAACCTTCGTGGGTTACAGAAGCAGCACCCAGTTCCATGTGTTTGAACTG ACAAGACAGCTGCCCCGATTCACCATGTATGCATTGACAAGCCCAGATGCAGCTAGCGAGCCTGTCAGCT ACGTGAACTTCAGTGTTGCCGAGCGCACACAGAGGATGGTCACGTGGCTCAACCAGAACTTTCTGTTGCC AGAAGACAGTAATGTTCAGAATTCCCCGTTTCACGTCTGTTTCACATCGTTACGCAATGGTGGCCAGCTC TACATAAAAATGAAACAAAGTGGTGAGATCACTGTGAATACTGACGATATTGATCTAGCTGGTGACATCA TCCAGTCGATCGCGTCCTTTTTTGCTATTGAAGACCTTCAGGTAGAAGCAGATTTCCCTGTGTACTTCGA GGAACTACGGAAAGTGCTGGTGAAGGTGGACGAGTATCATTCAGTGCATCAAAAGCTCAGCGCCGACATG GCTGATAATTCTAACCTCATCCGAAGTTTGCTGGTCAGAGCAGAGGATGCTCGGCTGATGAGGGACATGA AAACAATGAAGAGTCGTTACATGGAGCTCTATGACCTTAATAAAGATTTGCTGAATGGATACAAGATTCG CTGTAACAACCACACAGAACTGCTAGGAAACCTGAAGGCGGTGAACCAAGCAATCCAAAGAGCAGGCCGT CTGCGCGTTGGAAAGCCAAAGAACCAGGTGATCAGTGCTTGTCGGGATGCGATTCGAAGCAACAACATCA ACACCCTCTTCAGAATCATGCGAGTGGGCACTGCTCCTTCATAGGAGACAGGGAAAGAGGAAGGAAGCCC CTGCAAAAGGTCTAGACACGGAGCCTGGCTGGCTCCCGGTCACAGCCTGGGCATCCAGCTGCTAAGGATG AAACCATGCTAACTGATACAGCTAAGGACAAAGCACTCCGGCTGAAAGCCTCCTTCATTGACATCAGTGG TGTTGCTGATTTGTATCTTAAAATATATCACCATTTCAGAATTTAACTTGTTTTTGTACATAAAGTTAGT GTATGAATTTACCCCCCCCCCGAAATTGTTACTGAAATTTAAAATGGAGTACCTACTTTTTCATTAAAAA TAAAAGGCACTGGTCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5066846 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5066846 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003014 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source