EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10782

Otx2 orthodenticle homolog 2 (Drosophila) ( MGI:97451)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001203. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001203_01	euxassay_001203_02	euxassay_001203_03	euxassay_001203_04

euxassay_001203_05	euxassay_001203_06	euxassay_001203_07	euxassay_001203_08	euxassay_001203_09

euxassay_001203_10	euxassay_001203_11	euxassay_001203_12	euxassay_001203_13	euxassay_001203_14

euxassay_001203_15	euxassay_001203_16	euxassay_001203_17	euxassay_001203_18	euxassay_001203_19

euxassay_001203_20	euxassay_001203_21	euxassay_001203_22	euxassay_001203_23	euxassay_001203_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10782_wholemount_strong.wlz

10782_wholemount_moderate.wlz

10782_wholemount_weak.wlz

10782_wholemount_possible.wlz

10782_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10782_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 weak expression: see section 07 08 16

lateral ventricle choroid plexus

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 09 10 14 15

telencephalon ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 weak expression: see section 17 18 19

4th ventricle

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

midbrain mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 14 15 16 17 18 19 20

midbrain marginal layer

moderate

regional

moderate expression: see section 09 10 11 14 15 16 17

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

cochlea

strong

regional

strong expression: see section 04 05 06 07 08 09 16 17 18 19 20 21

neural retina

moderate

regional

moderate expression: see section 21 22 23 24 weak expression: see section 01 02 03

external naris

strong

regional

strong expression: see section 09 10 15 16

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 11 13 14 15 16 17 moderate expression: see section 18 weak expression: see section 06

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4347
Entity Detected:	Otx2, orthodenticle homolog 2 (Drosophila) ( MGI:97451)
Sequence:	sense strand is shown >T4347 CAGGTTTATCTGGTCTCACTCCATCCCCTCTAGTTTTGGAGCTGCTGGGGGGTGGGGGGGACGGCGGGGG TGGGGGACGCATCTGCAACTCCTTTAAAAGCCTGTGCCCAGCGTCTCCCGGGTTCTTTTTAGTTAGTGCT GGAACGTGGAGGAAGCTGCTCCCTCCGAAGCAGTAAACCAGCATTTCTGTTTGTTTGTTTGCTTTGCCCT TAGTTCCGTCACTCCAAATCTACCCACCAAGGACCCTGACCCTGTCCACTCCAGGCGAATCGAGACCGTC CGGCTGGGTCCCCCCAATTTGGGCCGACTTTGCGCCTCCAAACAACCTTAGCATGATGTCTTATCTAAAG CAACCGCCTTACGCAGTCAATGGGCTGAGTCTGACCACTTCGGGTATGGACTTGCTGCATCCCTCCGTGG GCTACCCCGCCACCCCCCGGAAACAGCGAAGGGAGAGGACGACATTTACTAGGGCACAGCTCGACGTTCT GGAAGCTCTGTTTGCCAAGACCCGGTACCCAGACATCTTCATGAGGGAAGAGGTGGCACTGAAAATCAAC TTGCCAGAATCCAGGGTGCAGGTATGGTTTAAGAATCGAAGAGCTAAGTGCCGCCAACAGCAGCAGCAGC AGCAGAATGGAGGTCAGAACAAAGTGAGGCCTGCCAAGAAGAAGAGCTCTCCAGCTCGGGAAGTGAGTTC AGAGAGTGGAACAAGTGGCCAGTTCAGTCCCCCCTCTAGTACCTCAGTCCCAACCATTGCCAGCAGCAGT GCTCCAGTGTCTATCTGGAGCCCAGCGTCCATCTCCCCACTGTCTGACCCCTTGTCCACTTCCTCCTCCT GCATGCAGAGGTCCTATCCCATGACCTATACTCAGGCTTCAGGTTATAGTCAAGGCTATGCTGGCTCAAC TTCCTACTTTGGGGGCATGGACTGTGGATCTTATTTGACCCCTATGCATCACCAGCTTCCTGGACCAGGG GCCACACTCAGTCCCATGGGTACCAATGCTGTTACCAGCCATCTCAATCAGTCCCCAGCTTCTCTTTCCA CCCAGGGATATGGAGCTTCAAGCTTGGGTTTTAACTCAACCACTGATTGCTTGGATTATAAGGACCAAAC TGCCTCTTGGAAGCTTAACTTCAATGCTGACTGCTTGGATTATAAAGATCAGACGTCCTCATGGAAATTC CAGGTTTTGTGAAGACCTGTAGAAGCTATTTTTGTGGGTGATTTTTAAATATGCTGGGCTGAACATTCCA GTTTTAGCCAGGCATTGGTTAAAAAAGTTAGATGGAACGATGCTCTCAGACTCCTGATCAAAGTTACCGA GAGGCATAGAAGGAAAAAGGAAGGGGCCTTAGAAGGGTCCATCAACCAGCAACCTGAAATGGACAAACCA ATCTACTTAAGATTCTGTTATAGTTCTAGATCATTGGTTTCCTGATTTGCAAATGATTGATCAAATATAT TCTAGCGACATGCAACCAAATACCACTCAAAACAAAAATCCAGCAAAACTGAGTTGTGAGGGAAGGGAGG GAAGGTCATGGCCTTCAAAGCAGAGGTGATCCGGTGTTTTAGCCAATCTTTGGTTGAATCTTAGGAATGG ACAATGTCCCAGGCTCATTCACGTTTCATGACCAACAGGTAGTTGGCACTGAAAAACTTTTCAGGGCTGT GTGGATTGTGCGACTGATTGTCCTAGATGCACTACTTTATTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4527414 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4527414 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10781 same embryo
	EurExpress:euxassay_001203 same experiment
	MGI:4827008 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source