EMAGE mouse gene expression spatial database

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EMAGE:10812

Tmed3 transmembrane emp24 domain containing 3 ( MGI:1913361)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001258. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001258_01	euxassay_001258_02	euxassay_001258_03	euxassay_001258_04

euxassay_001258_05	euxassay_001258_06	euxassay_001258_07	euxassay_001258_08	euxassay_001258_09

euxassay_001258_10	euxassay_001258_11	euxassay_001258_12	euxassay_001258_13	euxassay_001258_14

euxassay_001258_15	euxassay_001258_16	euxassay_001258_17	euxassay_001258_18	euxassay_001258_19

euxassay_001258_20	euxassay_001258_21	euxassay_001258_22	euxassay_001258_23	euxassay_001258_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10812_wholemount_strong.wlz

10812_wholemount_moderate.wlz

10812_wholemount_weak.wlz

10812_wholemount_possible.wlz

10812_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10812_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 03 04 05 06 07 19 20 21 22 23 24

pancreas

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

nasal capsule

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 14 15 weak expression: see section 16 17 18

lower jaw molar

moderate

regional

moderate expression: see section 06 07 08 09 10 19 20 21 22

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 14 15 weak expression: see section 16 17 18

upper jaw molar

moderate

regional

moderate expression: see section 06 07 08 09 10 19 20 21 22 weak expression: see section 18

axial skeleton

weak

regional

weak expression: see section 12 13 14 15 16

chondrocranium

moderate

regional

moderate expression: see section 01 02 03 04 05 23 24 weak expression: see section 06 07 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1126
Entity Detected:	Tmed3, transmembrane emp24 domain containing 3 ( MGI:1913361)
Sequence:	sense strand is shown >T1126 GCTCCGCACGCTTCATCCTTCCAGATGCTGCTACAGCTGCTGCTTCTGCTCCTGCTCCGGGCCGAGCCAC TGCGGAGCGCTGAGCTCACCTTCGAGCTGCCGGACAACGCCAAGCAGTGCTTCCACGAAGAGGTGGAGCA GGGCGTGAAGTTCTCTCTGGATTACCAGGTTATCACTGGAGGCCACTACGATGTGGACTGCTATGTGGAA GACCCCAGGGGCAACGTCATCTACAGGGAGACCAAGAAGCAGTATGACAGCTTCACGTACAAGACAGAGG CCAAGGGCGTCTACCGGTTTTGCTTCAGTAATGAGTTCTCCACCTTCTCTCATAAGACCGTCTACTTTGA CTTCCAAGTGGGTGACGAGCCCCCCATTCTCCCAGACATGGGGAACAGGGTCACGGCTCTCACTCAGATG GAATCTGCCTGTGTAACTATTCACGAGGCTCTGAAGACTGTGATCGACT
Notes:	The probe template was PCR amplified from IMAGE:2135538 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2135538 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10811 same embryo
	EMAGE:10809 same embryo
	EMAGE:10807 same embryo
	EMAGE:10808 same embryo
	EMAGE:10806 same embryo
	EMAGE:10810 same embryo
	EurExpress:euxassay_001258 same experiment
	MGI:4828760 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source