EMAGE mouse gene expression spatial database

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EMAGE:10846

Cmtm3 CKLF-like MARVEL transmembrane domain containing 3 ( MGI:2447162)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003403. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003403_01	euxassay_003403_02	euxassay_003403_03	euxassay_003403_04

euxassay_003403_05	euxassay_003403_06	euxassay_003403_07	euxassay_003403_08	euxassay_003403_09

euxassay_003403_10	euxassay_003403_11	euxassay_003403_12	euxassay_003403_13	euxassay_003403_14

euxassay_003403_15	euxassay_003403_16	euxassay_003403_17	euxassay_003403_18	euxassay_003403_19

euxassay_003403_20	euxassay_003403_21	euxassay_003403_22	euxassay_003403_23	euxassay_003403_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10846_wholemount_strong.wlz

10846_wholemount_moderate.wlz

10846_wholemount_weak.wlz

10846_wholemount_possible.wlz

10846_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10846_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5940
Entity Detected:	Cmtm3, CKLF-like MARVEL transmembrane domain containing 3 ( MGI:2447162)
Sequence:	sense strand is shown >T5940 CCGCTTCCCTCCAGGGCGGTGGGTGAGGCAGTGAGGCCCAGGCTCGACCAGATAGCGCCGCTATGTGGCC CCCGGACGCAGAGCCAGAGCCCGATCCAGAGTCCGCGCATGGCCCCCGAAGTGGCAGGACAGTGCCGGGA CTCCGAGCCCTGTTGCCCGCGCGCGCCTTCCTCTGCTCTCTCAAAGGCCGCCTCCTGCTGGCCGAGTCGG GTCTTTCATTCATCACCTTCATCTGCTACGTGGTGTCCTCCGCATCTGCCTTCCTCACAGTGCCTCTGCT GGAGTTTCTGTTGGCCGTTTACTTCCTCTTTGCTGATGCCATGCAGCTGAATGACAAATGGCAGGGTTTG TGCTGGCCCATGATGGACTTCCTGCGCTGTGTCACCGCTGCCCTCATCTACTTCGTCATCTCCATCACTG CTGTCGCCAAATACTCAGATGGGGCTTACAAAGCAGCTGGAGTTTTTGGCTTTTTTGCCACAATTGTGTT CGCAATTGACTTCTACCTGATCTTTAATGAAGTGGCCAAATTCCTCAAGCAAGGAGACTCTGGGAATGAG ACCACAGCCCATAGGACAGAAGAAGAGAACTCCAACTCGGACTCAGACTCTGACTAAAGGCCTGTCTGAG CTTAACAACTAGAGCCACTCGGCCCTCTCACCCACGCTGTCCTGACGGGGCTCCTGGAGCATGGCAGGAA TGCCCATGGGAGAACAGACCGAGGAGCCCGTCTCTCAAGATTACACTCCCGAGGCCTCACATTCAAAATT GGCTGTGCTGGCTTGGCACTGCTGAGCTAGCGTGGTAGGGCACGTTGACAAGCCTTCCTTCTCCCCTTTC TGTCCTTGAGCCCCTTTAACCTCACTGCCCCCACCGAAGCACACATCAAACGGAGCCAATCACTACAGCC AATCATGTGGCAGCATGGGGGTGGGGTGGGACTTTCTGTTACTAACAGGGACAGATAAAACCACAGCAGG CTGGACCACCAGCCCCAAACCTGCACTGCATTGAGATGACTTTCAGGAACCTTTTATTCAAGAAGTTTCC CTCCTCACTGTGAAATAGGGAGAAACCTAGCTAGCCAAGTCTTCCATTAGAGGTCTGAGGCCTTTTCAGA AACGGACCCAAAGATTTGTTTACTAGGGCCCAGAGACAGGGAGCCAACCAGTATAGTGGCAGTGACCAAG TCACAAGTGGCTGGGCAGGCCCAGCCAAGCACTCTCTTCCTGGGGACCCTAAAAGACAAAGTGCTTCCTC TGGACACCTGGGCAGGACCACTGGTGACATGGACTTCTCTGTAGTCCCTTGGGGGCGGTTACTGTGTGTG TCTTTGATGGTGTTTTCTAACTTTTTTATATTTTTTTAATACAAAAACAATGTAACAAATGGTCTTTTCT GTAACCTAAGAGGCCCCATGAATAAGGGCCCATGTCTTGGGCATTTGTAACCTTTTTATCTTGCATGTGA ATTCCCTCCCTTTAAAAAAAAAAGTCACAATGGATTAAAACAAACTGACTACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3483351 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3483351 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10851 same embryo
	EMAGE:10848 same embryo
	EMAGE:10850 same embryo
	EMAGE:10849 same embryo
	EMAGE:10847 same embryo
	EurExpress:euxassay_003403 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source