EMAGE mouse gene expression spatial database

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EMAGE:10847

Taf11 TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor ( MGI:1916026)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003454. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003454_01	euxassay_003454_02	euxassay_003454_03	euxassay_003454_04

euxassay_003454_05	euxassay_003454_06	euxassay_003454_07	euxassay_003454_08	euxassay_003454_09

euxassay_003454_10	euxassay_003454_11	euxassay_003454_12	euxassay_003454_13	euxassay_003454_14

euxassay_003454_15	euxassay_003454_16	euxassay_003454_17	euxassay_003454_18	euxassay_003454_19

euxassay_003454_20	euxassay_003454_21	euxassay_003454_22	euxassay_003454_23	euxassay_003454_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10847_wholemount_strong.wlz

10847_wholemount_moderate.wlz

10847_wholemount_weak.wlz

10847_wholemount_possible.wlz

10847_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10847_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5941
Entity Detected:	Taf11, TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor ( MGI:1916026)
Sequence:	sense strand is shown >T5941 GCTGCCATCGCCTCTGACCCCCTGCTCTCGCGATGGACAACCTCGGGGAGTCGCCCACAGACAAAGGCGG GGAGCCAGGGGAGTCAGAGGAGACGAGGGCTACTCCTGGGGCTTTGGTGGCAGCCGACACTGAGGGAATC CCGGAGGAAACCGACAGGGATGGAGACGCGGACTTGAAAGAAGCCGCGGCGGAGGAAAGCGAGCTCAAGA GTCAGGATGTCTCAGATGTAACAGCAGCCGAGAGGGAAAACTCGTCCTTACTTACTCCTGCAGCCAAAAA GCTGAAACTAGACATTAAGGACAAGAAGGAGAAGAAGCAGAAGGTGGACGAAGATGAGATTCAGAAGATG CAAATCCTGGTCTCTTCTTTTTCTGAGGAGCAGCTGAACCGTTATGAAATGTATCGCAGGTCAGCTTTCC CCAAGGCGGCCATTAAAAGGCTGATCCAGTCCATCACTGGCACCTCTGTGTCTCAGAATGTTGTGATTGC CATGTCTGGCATTTCTAAGGTTTTTGTCGGGGAGGTGGTAGAAGAAGCCCTGGACGTGTGTGAGAAATGG GGAGAAACGCCACCCCTGCAGCCCAAGCACATGAGGGAGGCCGTCCGGAGGTTGAAGTCCAAGGGGCAAA TCCCCAACTCAAAGCACAAGAAGATCACCTTCTTCTAAATCTAAGGGAGACTGTTCCAGACTTCTCAGAG TCGGGCTTCTGTCCTGGGGCTTTGGTGGTGGTGAGCTCGGGACGCAGGATGCCGATTTTAATGTTTCGGT CACACCTACAAGGCATGCAGTGTGACTCAGACTTGCCTTCACTCAGTCATGGACTGTCCTCAGGCATGTT GAAGACTTTTACTGACTCTTGGCTAGTTTGAAGAAAAAATATATAGGGGCCTTTAGTATCTTCTGGAGAA GGAGATGGTTTCAGAGAAAAACAGAACAACTCTAGTGGCTTCATATTGAAACCTAAAGTTGCTAAGATTC AGGTTGTTTCTTGCTCTGGTTTTCAAAACATAAACTTGCGATTATCTGCGGTGAAGTCACCGTGACACGC TTCTCACCATTAACATTTCAGGTTCTCCTGTGGATTTTGTGAGGGATCA
Notes:	The probe template was PCR amplified from IMAGE:3483315 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3483315 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10851 same embryo
	EMAGE:10846 same embryo
	EMAGE:10848 same embryo
	EMAGE:10850 same embryo
	EMAGE:10849 same embryo
	EurExpress:euxassay_003454 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source