EMAGE mouse gene expression spatial database

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EMAGE:10852

Sorbs1 sorbin and SH3 domain containing 1 ( MGI:700014)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003610. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003610_01	euxassay_003610_02	euxassay_003610_03	euxassay_003610_04

euxassay_003610_05	euxassay_003610_06	euxassay_003610_07	euxassay_003610_08	euxassay_003610_09

euxassay_003610_10	euxassay_003610_11	euxassay_003610_12	euxassay_003610_13	euxassay_003610_14

euxassay_003610_15	euxassay_003610_16	euxassay_003610_17	euxassay_003610_18	euxassay_003610_19

euxassay_003610_20	euxassay_003610_21	euxassay_003610_22	euxassay_003610_23	euxassay_003610_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10852_wholemount_strong.wlz

10852_wholemount_moderate.wlz

10852_wholemount_weak.wlz

10852_wholemount_possible.wlz

10852_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10852_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23 24

forelimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 23 24

hindlimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 23 24

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 18 19 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 05 06 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 09 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 07 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 17 18

spinal cord

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 13 14 15 not examined expression: see section 16 17 18 19

otic capsule

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 15 16 17 18 19 20

lens

strong

regional

strong expression: see section 01 moderate expression: see section 02

retina

moderate

regional

moderate expression: see section 01 02 03 04 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13 15 16 17 18 19 20 21

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 13 14 15 16 17 18

nasal septum

moderate

regional

moderate expression: see section 14 15

heart

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 weak expression: see section 22

esophagus

moderate

regional

moderate expression: see section 11 12

tongue

weak

regional

weak expression: see section 11 12 13 14 15 16 17

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09

midgut

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

lower jaw incisor

moderate

regional

moderate expression: see section 13 14 weak expression: see section 15

liver

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

bladder

moderate

regional

moderate expression: see section 11 12 13 14 15

metanephros

moderate

regional

moderate expression: see section 05 06 07 08 16 17 18

lung

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 not examined expression: see section 01

trachea

moderate

regional

moderate expression: see section 12

cervical vertebra

moderate

regional

moderate expression: see section 14 16

lumbar vertebra

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

sacral vertebral cartilage condensation

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

thoracic vertebra

moderate

regional

moderate expression: see section 14 16

not examined

regional

not examined expression: see section 01

orbito-sphenoid

strong

regional

strong expression: see section 01 02 moderate expression: see section 03 04 05 07 08 09 weak expression: see section 22 23

sternum

moderate

regional

moderate expression: see section 13 14

axial skeleton tail region

moderate

regional

moderate expression: see section 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6700
Entity Detected:	Sorbs1, sorbin and SH3 domain containing 1 ( MGI:700014)
Sequence:	sense strand is shown >T6700 CCACGCGCCGCGGGGCTGACTGTAGCGTGGAGCGCGAGCCGGGCTGGACGCGCGCAAGCCCTTGCCGGGG ACCCGCGAGGAGTTGGCATCCATTGGAAGACCTTGAGATCCTCTCAGCTCAGAACTCCAGGACCGATGCA TCTTCCCACCACCTTGAAGCACTGAGCCCTCCAGAGCTGCATCTGGGAAGACTCGCCTGCCTCCAGCATG AGTTCTGAATGTGATGTTGGAAGCTCTAAAGCTGTGGTGAATGGCTTGGCATCTGGCAACCATGGACCAG ACAAAGACATGGACCCTACCAAAATCTGCACTGGGAAAGGAACAGTGACTCTTCGGGCCTCGTCTTCCTA CAGGGGAACCCCAAGCAGCAGCCCTGTGAGCCCCCAGGAATCTCCGAAGCATGAAAGCAAGTCAGATGAA TGGAAACTTTCTTCCAGTGCAGATACCAATGGCAACGCCCAGCCCTCCCCACTTGCTGCCAAGGGCTATA GAAGTGTGCATCCCAGCCTTTCTGCTGACAAGCCCCAGGGTGCCACCTCCTCCAGCTCAGCCCCGTCTGA GGGCAGTCCTTTACTAAACGAAGTTTCTTCTTCCCACATTGAAACCGATTCCCAAGACTTCCCTCCAACA AGCAGACCTTCGTCTGCCTACCCCTCCACCACCATCGTCAACCCTACCATTGTGCTCCTGCAGCACAATC GAGAGCAGCAAAAGCGACTCAGTAGTCTTTCAGATCCTGCCTCAGAGAGAAGAGCGGGTGAGCAGGACCC AGTACCAACCCCAGCAGAACTCACTTCGCCCGGCAGGGCTTCTGAGAGAAGGGCAAAGGATGCTAGCAGA CGGGTGGTGAGGAGCGCACAGGACCTGAGCGATGTGTCTACAGATGAAGTGGGCATCCCACTCCGGAATA CCGAGCGATCGAAAGACTGGTACAAAACTATGTTTAAACAGATCCACAAACTGAACAGAGATGATGATTC TGATGTCCATTCCCCTCGATACTCCTTCTCTGATGACACAAAGTCTCCCCTTTCTGTGCCTCGCTCAAAA AGTGAGATGAACTACATCGAAGGGGAGAAAGTGGTTAAGAGGTCCGCCACACTCCCCCTCCCAGCCCGCT CTTCCTCACTCAAGTCCAGCCCGGAAAGAAACGACTGGGAGCCCCTAGATAAGAAAGTGGATACGAGAAA ATACCGAGCAGAGCCCAAAAGCATTTACGAATATCAGCCGGGCAAGTCTTCGGTCCTGACCAATGAGAAG ATGAGCTCAGCAGTCAGCCCGACTCCAGACATTACGTCAGAGCCTCCTGGATATATCTATTCTTCCAACT TCCATGCAGTGAAGAGAGAATCGGACGGGACCCCCGGGGGTCTCGCTAGCTTGGAGAATGAGAGGCAGAT CTATAAGAGTGTCTTGGAAGGTGGCGACATCCCTCTTCAGGGCCTCAGTGGGCTCAAGCGACCTTCCAGC TCAGCTTCCACTAAAGATTCAGAGTCACCAAGACATTTTATACCAGCTGATTACTTGGAGTCCACAGAAG AATTTATTCGGAGACGGCACGATGATAAAGAGATGAGACCTGCTCGAGCCAAATTTGACTTTAAAGCCCA GACCCTGAAGGAGCTGCCTCTGCAGAAGGGAGACGTTGTTTACATCTACAGACAGATTGACCAGAACTGG TATGAAGGTGAACACCATGGCCGGGTGGGAATCTTCCCACGCACCTATATCGAGCTTCTTCCTCCAGCTG AGAAGGCTCAGCCCAGAAAGTTGGCACCCGTACAAGTTTTGGAATATGGAGAAGCCATTGCAAAGTTTAA CTTTAATGGAGATACACAAGTAGAAATGTCTTTCCGAAAGGGGGAGAGGATCACGCTGCTCCGACAGGTG GATGAGAACTGGTATGAAGGGAGGATTCCTGGGACATCTCGCCAAGGCATTTTCCCTATCACCTATGTAG ATGTGCTTAAGAGGCCATTGGTGAAAACCCCTGTGGATTACATCGACCTGCCTTATTCTTCTTCCCCAAG TCGCAGTGCCACTGTGAGCCCACAGCAACCTCAAGCCCAGCAGCGAAGAGTCACCCCAGACAGGAGTCAG CCCTCACTGGATTTGTGTAGCTACCAAGCGTTATATAGTTATGTGCCACAGAACGATGATGAGTTGGAAC TCCGAGATGGAGATATTGTTGATGTCATGGAAAAATGTGACGATGGATGGTTTGTTGGCACTTCGAGAAG GACGAGGCAGTTTGGTACTTTTCCAGGCAACTATGTAAAACCTTTATATCTATAAGAAGACTAAAAAGCA CAGAGATTATTTTTTATCGGAGGATGAAGCATCATTCATGAACTGGTCTCTTTATTTAAGTACTGAGTCA GTAAGAAAACTAATGCAGTTGGTAAAGAAAGAATTCAAAGAAGGAACAGAGAAGTGTGTTTGAAACCCAT TGTGTATCAGGGATTAACTATCTGCTGAAGACATCTGTATTTACATGACTGCTTCTGGGAGCTGCTCTAG CCCCCGCTGCTTGGGGAATCTGATCTGGAGCATGTCCATGAGCAACATTAGCCAAAAAAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3602997 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3602997 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4828377 same experiment
	EurExpress:euxassay_003610 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source