EMAGE mouse gene expression spatial database

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EMAGE:10883

1700019G17Rik RIKEN cDNA 1700019G17 gene ( MGI:1922791)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003387. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003387_01	euxassay_003387_02	euxassay_003387_03	euxassay_003387_04

euxassay_003387_05	euxassay_003387_06	euxassay_003387_07	euxassay_003387_08	euxassay_003387_09

euxassay_003387_10	euxassay_003387_11	euxassay_003387_12	euxassay_003387_13	euxassay_003387_14

euxassay_003387_15	euxassay_003387_16	euxassay_003387_17	euxassay_003387_18	euxassay_003387_19

euxassay_003387_20	euxassay_003387_21	euxassay_003387_22	euxassay_003387_23	euxassay_003387_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10883_wholemount_strong.wlz

10883_wholemount_moderate.wlz

10883_wholemount_weak.wlz

10883_wholemount_possible.wlz

10883_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10883_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6258
Entity Detected:	1700019G17Rik, RIKEN cDNA 1700019G17 gene ( MGI:1922791)
Sequence:	sense strand is shown >T6258 CTTGGGCCAGGAGTCTGCTCACCATCTCAGGAGAAAAGTGTCCGCAAGGCAGGAGAAGCATTCGCTGGGT CAGTTCCTGAGAGCGGAGCTCACCCTTAGTCTCGGTCGTCTCAGAAAGATCTTCCCCACGGAAACAAGTC CGCCACTTCCGGAAGACACAGTTCCCGCCCAAGTAGTCAGCCAATGAAGTGCCAGGCTAGATCTATGCTC TAGAGGTTCATCAGACCCTGGTAGATTCTCCCTGGATGTCATTGGAGCCTGAAGCCATCCTGGACACTTG GAACTGTGGTACTACACGAGAGGACAGAGAGTCTGAATCATACCAGTTGCTGGAAAGAGGATTTGTTGAA ACTTGCACTTGGGAACACAGGAGTTTTCCAGTCTGGACCCTGAAGAGGAAACAGAAGATCTCAGAACAGC ACATCTTTCCACAGTGTGGCACCTCAGTTCCCAAAGGGCTCAGGGAAGCTGTGCAAGAAGGTCTGGATGT CCCTTGTGATCACTGATACTTGAGAGCCAGAAGTCTCCCCATGGCTTTTTATCACATCCGACAGTACCAG GAGAAGGACCACAAAAAGGTCCTGGAATTGTTCTCCAGGGGCATGAAGGAACATGTCCCTGCTGCCTTCC ACCATATGCTGACACTACCTCAGACTCTCCTGCTTTTACCTGGGGTGCCTGTGACCATAGTCCTAGTGTC TGGCTCCTGGCTCCTGGCCACAGTATGCAGCTTTCTCTTTCTCCTTTGCCTGCGGCTCCTTATCTGGCTT TCTTGGAGAAATTACGTGGCTACAAGTTTGCAAGCTGACCTGGCTGACATCACCAAGTCTTACCTGAATG CACATGGCTCCTTCTGGGTGGCTGAGTCTGGAGACCAGGTAGTTGGCATGGTGGGTGCTCGGCCAGTCAA GGACCCTCCATTAGGGAAGAAGCAGATGCAGCTCTTTCGCCTGTCTGTGTCCTCACAGCATCGAGGACAG GGGATAGCGAAAGCACTAGTCAGAACTGTCCTCTGGTTTGCTCGGGACCAGGACTACAGTGATGTTGTCC TTGAGACCAGTTGTGTGCAGTACAGTGCTGTGGCTCTCTACCAGGCCATGGGCTTCCGGAAAACAGACCA GTACTTTGTGAGCATAGCCACAAGGTTAATGGGTCTTTCTATCCTTCATTTCACCTACTCTCTCCCTTTT GCTTGGGGATCAGGGATGTGACCTGTTATCCTTTGTTTGCCTTGGAGGCTCCAGTTCACTCTGCTGTAGG AACAAGCCATCCCTGACAAGTTCCTGCTACCAATCCCAAGGGCTCCCTTTGCACTCCTTTCAGATGCAGC CAGGCTGGTGGTGACCTGTGGGTGTTGGCTCCATTCTGCCTTTTTAAGTATCTCTCTTTCGTCTTTCTAG TGCCATGATAAGATAGCACAAAACCCAACTGAGTCCATCCTGCACACAGTTCACTTAGGAATCTGTTAGC TGTCAGTATCAGCTAGGTGGTCTCAAGACTTCAAGGCAAGCAACCTTGCTGAGCTGAAGTTCCAGCAGCT CACAGGATTTAAATCTAAGACTTGTGTAGAAATGGAGCCTCCACTGGTAGAGGCACAGCACAGATCAGGG TGGCTGCACTGAGAGCACTGGAGCAACGAAGCCTGTAACTTACACCTAAGCCACCTCTGTGCAGAGGGAG GAAGGGGACCTCTGATGCAGTGAGAGTAACTTTGAATGGCCTCTCCTGGTGGAATGATCCCTCCTGGCCT CTTGGTCCCAGTCTTACGGGAAAGCACAATTAAATGCATGAAATGTACTTGGGGATTCATGAAGATGAAA TGGGATCAGGAATTGTATGAATATGTCACCCTAGGGAAAATGCCCAGACTAATTGGCACTCGAGTAGGTT GGACATCAGTGGGCTTCCAGTGCTCCTGGATTTCCTCTGTAAAAAGTTAAAATGGCAGTTCAGGAGTTGG AACAGCACTGTAGAGTGGAGAAAGGCAGTCTCAGAACTAATAAAGTTGTTGGCTGAAGATGACCCTGCTA GGCATAAGAGCCTTCCAGAATTGTTAAGGAAAGGCCCCGAGGGCCAACGAATCACAGATGTGGTTTCCAC TGCTTTTGGTTGCATGCTGGAACTGGAGAACTCCTTGCTGAGGACATGACCTGTTTGGTTTCAGGACACA GAAGAATCAACAGAGATTGACCTGGAACAGCCTTGTGTGCTCGCTGGGTTTCTAGTGCCAAGAGGCTCGA TCCAGGCTGCTGAGGGACAGTTATTGAGACAGTCTTGCTCCCTGTGCTGTGTTGTTACATGCCGGGAAGA TGTATGTTGTAGTGTTATAGTGGCATTGTTATGTTTGCACACACATACTTTCTGATTAAGGCCTCCACAG GTGGATATTCCTATTGATTATTCTAAATCAAGACAAAATTATGTGATGTGCAGGCCATAGGCCATAGTAA GGAAACTACTACTATTGTTTTAGTTTTTATTAAGCAGGTATAGTATATCAGCTTGCCTTCAAAACATTTG TGTTCATGCCCAATGACTGCTGTTCCAGTGTCACTTGTAATTGGGAGCCTTTTCACAGTGGGAGATGGTC ACTCCAAAGACTCATAACTTGTACAATTGCAGAGAATACGTGACTGATCCTTTGATATAGCCCAATATTG GGCCATAGATGGAAGAAAATAGATGTATATGTCCAAGGCTTGGAAGAGTTATGGAAGCGGATGCATAAAG GTTTAAGATCTGGAGTGGGTGGGATACCCTGTATCTCTCTGGTCTGAATTGAAACTTCCCCTTTTAGGGG GCTGACAGAGGCTCAGGAGACTCTGGAGAGCGTTCCTCTCTGATGTCACCCAGGCAGTAAACATTTATCA GCAGAGAGAAGCCTCTTAGGAACCCCAGGGTTGGAGGTTTGTAAGTCATTACCAATGCAATGGCCATACT GCCTGGGCAACCCGTGCTCCTGTAAGTAATCCCTCAGCTGTGCTCCTGTGCTCCAATAAACTGTTTCATC AAACTTGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3501234 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3501234 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10887 same embryo
	EMAGE:10888 same embryo
	EMAGE:10886 same embryo
	EMAGE:10884 same embryo
	EMAGE:10885 same embryo
	EurExpress:euxassay_003387 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source