EMAGE mouse gene expression spatial database

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EMAGE:10884

Alcam activated leukocyte cell adhesion molecule ( MGI:1313266)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003463. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003463_01	euxassay_003463_02	euxassay_003463_03	euxassay_003463_04

euxassay_003463_05	euxassay_003463_06	euxassay_003463_07	euxassay_003463_08	euxassay_003463_09

euxassay_003463_10	euxassay_003463_11	euxassay_003463_12	euxassay_003463_13	euxassay_003463_14

euxassay_003463_15	euxassay_003463_16	euxassay_003463_17	euxassay_003463_18	euxassay_003463_19

euxassay_003463_20	euxassay_003463_21	euxassay_003463_22	euxassay_003463_23	euxassay_003463_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10884_wholemount_strong.wlz

10884_wholemount_moderate.wlz

10884_wholemount_weak.wlz

10884_wholemount_possible.wlz

10884_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10884_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6261
Entity Detected:	Alcam, activated leukocyte cell adhesion molecule ( MGI:1313266)
Sequence:	sense strand is shown >T6261 CGTGGAGGAAAGTTGCGTGTGGCACAGACCCGACGGCGCAGCGCCACAGTCCCCGGGGGACCGTGTGTCT TGGAAAAAAGTCGCTGTCCCCCTAAGGCGAGACCCACAAGCGAGCGGGCCCCGTGGGCCCGGGGACGACG CCCCCTCCTGCGGCGTGGACTTTGTCGGTGGCCTTCTAGGAGGAGGAATATGGCATCTAAGGTGTCCCCT TCTTGCCGTCTGGTTTTCTGCCTCTTGATCTCCGCCGCGGTCCTCAGACCAGGCCTTGGATGGTACACTG TCAACTCAGCATATGGAGACACCATTGTCATGCCTTGCCGTCTCGATGTACCTCAGAACCTCATGTTTGG CAAATGGAAATATGAAAAGCCCGATGGGTCCCCAGTATTTATTGCATTCCGATCTTCTACGAAGAAAAGT GTGCAGTATGATGATGTCCCAGAGTACAAGGACAGACTGAGCCTCTCAGAAAACTACACTTTGTCTATCG CCAATGCTAAGATCAGTGATGAGAAGAGATTTGTGTGCATGCTAGTGACCGAGGACAATGTGTTTGAAGC ACCTACCCTAGTCAAGGTGTTCAAGCAACCATCTAAACCTGAAATTGTAAACAAAGCACCCTTTCTTGAA ACAGATCAGCTGAAAAAGTTAGGTGACTGCATTTCAAGAGACAGTTACCCAGATGGCAACATCACATGGT ACAGGAATGGCAAAGTGCTGCAGCCTGTGGAAGGAGAGGTGGCCATACTTTTTAAAAAGGAAATTGATCC AGGAACTCAGTTGTATACAGTGACTTCCTCCTTGGAGTACAAGACAACCAGATCTGACATACAAATGCCA TTCACCTGTTCTGTGACATATTATGGACCCTCCGGCCAGAAAACAATTTATTCCGAACAAGAAATCTTTG ATATTTACTATCCTACAGAGCAGGTGACAATACAAGTGCTGCCACCAAAAAATGCCATCAAAGAAGGGGA CAACATCACCCTTCAGTGTTTGGGGAATGGCAACCCACCTCCTGAGGAGTTCATGTTTTACTTACCAGGA CAGCCTGAAGGCATAAGAAGCTCAAACACTTACACACTGACAGATGTGAGACGCAATGCCACAGGGGACT ACAAATGCTCACTCATTGACAAAAGGAACATGGCGGCTTCAACGACCATCACAGTTCACTACTTGGATTT ATCCTTAAACCCAAGTGGGGAGGTGACCAAGCAGATTGGCGATACCCTGCCTGTGTCGTGCACAATATCT GCAAGTAGGAACGCGACTGTGGTGTGGATGAAGGATAACATCCGGCTCCGGTCCAGCCCTTCCTTTTCCA GTCTTCATTATCAGGATGCTGGGAACTATGTCTGTGAAACTGCTCTTCAGGAGGTTGAGGGACTGAAGAA AAGGGAGTCGCTGACACTCATTGTAGAAGGGAAACCTCAAATCAAAATGACTAAGAAAACTGACCCCAGT GGACTGTCTAAAACTATAATCTGCCACGTGGAAGGGTTTCCAAAGCCAGCTATACATTGGACCATTACCG GCAGTGGGAGCGTCATAAACCAAACAGAGGAGTCTCCTTATATTAATGGCAGGTATTATAGTAAAATTAT CATTTCCCCTGAGGAGAATGTTACATTAACTTGCACAGCAGAAAACCAACTGGAGAGAACAGTAAACTCC CTGAATGTCTCTGCGATAAGTATTCCAGAGCACGATGAGGCAGACGATATAAGTGATGAAAACAGAGAAA AGGTGAATGACCAGGCAAAACTGATTGTGGGAATTGTTGTTGGTCTCCTCCTCGCTGCCCTCGTTGCTGG TGTCGTCTACTGGCTGTACATGAAGAAGTCCAAAACTGCATCAAAACATGTAAACAAGGACCTTGGTAAT ATGGAGGAAAATAAAAAGCTGGAAGAAAACAATCACAAAACAGAAGCCTAAGAGAGGAGCGGATTGGATA GCCTGAGGAAAAAACATATAAGCAAATTGAAGCATGAACGTGGATTGTATTTAAGACATATACAAAGACA AATGACAGCAATTCATGGTTCAAGTATAAAGCAGTTCATTCTACCAAGCTGTCACAGGATCTTAGAGAAT TATCTCAAGTAAAACAGTGAAATAAAGTTATAAAATAAAATTACAAGATTTGGCAGCCATGATAATAGGT CATATGTTGTATTTCGTTTGGTTTTTTTTTCTGTAAATGTCTGCACTAAGGGTTTCTTTCAGTTTGCCTT TTATGTAAATTTTTTACGTAGCTATTTTTATACACTGTAAGCTTTGTTCTGGGAGTGGCTGTTAATTGGA TGTATAATGTAACGTTTTATTTCAATTGTTTATATGGATAGTCTGAGCAGGTACGGTTCTGATTCTGATT GCTATCAATAACACCCCATACTTTCTTTGAACATACTTTGACCTGAAGCTCAAAGGTGGCAGTTGTGAGA TTGGGACACATATCTTGCCCAATCAGAAGCTGTTTCTTATCTTGAGAGTTAGGAGCACCATAGGATATGA TGGTCTATGTGCTGCCAACAGGCTCCCCGAAGCGAGTCACAAACACATACAGCAACACTACTTGAGAATG GTACTCCCAAACTGACAATTTTTTCTTTTCTGAAAAAGATATACACATAGTGTGTATCACATTTACTTAT TGACACTTACTAAGAAATGACATTCTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3501215 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3501215 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10883 same embryo
	EMAGE:10887 same embryo
	EMAGE:10888 same embryo
	EMAGE:10886 same embryo
	EMAGE:10885 same embryo
	EurExpress:euxassay_003463 same experiment
	MGI:4823079 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source