EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10885

Trim59 tripartite motif-containing 59 ( MGI:1914199)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003499. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003499_01	euxassay_003499_02	euxassay_003499_03	euxassay_003499_04

euxassay_003499_05	euxassay_003499_06	euxassay_003499_07	euxassay_003499_08	euxassay_003499_09

euxassay_003499_10	euxassay_003499_11	euxassay_003499_12	euxassay_003499_13	euxassay_003499_14

euxassay_003499_15	euxassay_003499_16	euxassay_003499_17	euxassay_003499_18	euxassay_003499_19

euxassay_003499_20	euxassay_003499_21	euxassay_003499_22	euxassay_003499_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10885_wholemount_strong.wlz

10885_wholemount_moderate.wlz

10885_wholemount_weak.wlz

10885_wholemount_possible.wlz

10885_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10885_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6260
Entity Detected:	Trim59, tripartite motif-containing 59 ( MGI:1914199)
Sequence:	sense strand is shown >T6260 GCCCGCCTCGCCCGCCTCGCCCGCCTCGCCCGCCTCGCCCGCCTCGCGAATAACAAAGGATCCTGGAAAA GCTGGAGAAGATAAAGTACACCCAGTGTCCAGATCAGGAGATTGACAGACCAGGAAATGCACAATTTTGA GGAGGAGTTAACGTGTCCCATCTGTTACAGCATCTTTGAAGATCCCCGTGTACTACCATGCTCTCATACA TTTTGTAGAAACTGTTTGGAAAATGTTCTTCAGGCATCTGGTAACTTTTACATATGGAGGCCTCTGCGAA TTCCACTCAAGTGTCCTAACTGCAGAAGTATTATTGAAATTGCTTCTACTGGCATAGAATCCTTACCTGT AAACTTTGCATTACGAGCAATTATTGAAAAGTACCAGCAAGAAGACCACCCAGATGTTGTCACCTGCCCT GAACACTACAGGCAACCATTAAATGTTTATTGTCTACTAGATAAGAAATTAGTTTGTGGCCATTGTCTTA CTATAGGCCAACATCATGGCCATCCTATAGATGACCTTCAAAGTGCCTATCTGAAAGAAAAGGATACACC TCAGAAGTTGTTGAAACAGTTAACTGACACACATTGGACAGATATCACTCGCCTTATTGAAAAGCTTGAA GAACAGAAATGTCATTCTGAGAAAATAGTTCAAGGTGATAAGGAAGTTGTTCTCCAGTATTTTAAGGAGC TAACTGATACATTAGAACAGAAAAAAAAGTATTTTTTGGCAGCTCTTTGTGATGTTGGCAAAATGATTAA TCAAGAATACACTCCACAGATTCAAGGAATGAAGGAGATAAGGGAACAACAACTTGAATTAATGACAATA ACGACATCTTTACAAGATGAGTCTCCACTTAAATTTCTGGAAAAAATTGATGAAGTCCGCCAACGTGTGC AGATGTTGAAACAGAGACCACTTCCTGAGGTCCAGCCTGTTGAAATTTATCCTCGAGTAAGCAATGTATT AAAAGAAGAGTGGAGCAGAATAGAAATTGGACGTATTAAGAAAGCTGTTATTCCTGAAATGAGAGTTTCT TCAAAAAGAACGCCATGTTCCTGGTCTGATAATGATGAAAAAGAAATGGAACTTTTTAAAATTTTAAACA TTGCTATAGTTTCACTCATTTCAGTGATACTAATGTTGATACTCCTTTTCAACCATCACATAATAACATT CTTAAATGAAATCACTTCAATATGTTTCTCTGAAGTCTTTCTCTCTGTTTACCAGAGTTTATCTAAGAAC TTATATGACTTAAACAATACAGTGTGTTACACTTTATATTTGTTAAAGGAATTTATGTGGAAAATAGTTT CTCGTTGAAAACCTAGAATTTCCAGTTTTATGTGGGCTTCTGTATAGCAGAGGCTAAGCATTGATTGCTA GATGGAAAGTCAGGGTGGCATAAACAGCACATCAACTTCACATCTTACAACAGATGCAGAGAAATACGAT AAACTTCCCATGCTTGGCTACAAGTGCTGTCAGAAATGAGACATTACCTGAAAGAAGACTAAAATCAAAT TAGAAAATGACAACTTTTGGGTTGAATATTAATACCTTACCCTGTTTTTATTGGCTTTCTAGTTAATGTT TTATTCTGATTAATAGGTCTTTTTCTTTAACTGTTACATGTTATAGTGGCTAACTTGGCAGTTCTTTGTG TATGTAGATATAAGATTTTTAAACAAATGACAAGATCCTTGAGGTCCACAAGCCTATTTTGATGATTTGA AGCTTTCCAATGCACAGATGGAGAGGATTTATAAGTTGTTTTAAAAATCTAGGCCAGGATTTTTTAAACC TTTTTTTGGGGTCAAGGCTATTCATAGGTTTAGGAGCAGTCATCCCAAGTAGCACTGCTCGCTGCTTGTG GATAAACTGTCTAGTACACTTGAGATTAATTCATCCTTGATATCAGTGAGGTCTCAAAAACCCATCAGAA AGTGTCTGGACTGCTGGCTCTAGCACCACAAAGCAAATCAAGTACATATTTTGATTTTGTATTTTAGCCT TGTTTTTTTATGAAAACTATACGAAACGTACATTGGGACAATCATGTTTATGTGATTCATCTAATAGATT ATGATGTAAAAACTATATTCATCTTAAAATGTTCTCACCCTGTAGCTTGAGTACTGGGATTATGAGTTGA TGCTGTACCTGTTTAGTTTTAAAAGAGGTCATTAAGTCAGAACCTTTAATTTTGCTCACTTGACATGGAC TACCATGATGCATGTGGTAGAACTTTTGGTTCACAGCCATTGAAATCCCCTACACAGTGTTTTCCAGAAA CCCCTTATGAATGCTCTTGAATGGAGACTTTATTTCAAATAAAAGCTGTGTTTCTTAAGGTCAGAACAAT GCTGACAAGTTTTATCACTAAAGTCCACTTATCAGACTCAAAATCTAGGCATGGTAACAAAATGAGGTCA TTTTGAAGATGCAATGAAGGTGAGGGGAAATTATGTAAATATTAGCAAGCGAAGAATATTGGAACATTTT TCAAGGGGTTACTAGCTTCTTTGTAGAGATGAGGAAACAAAAGTGCCAGTGTTAGCAGGTGGGAAAGCTC ATTCTATTCTGCTTATAAACAACTTGAGTAGGCTGATCTATGTAATTAACGGAAACTTCTATTTCAGTAT GCCCAGTATTACATTGTAGGCTTGACGATTTTGTGTACACAATTATCTTTGTGCTCTGATATACTGTTTT ATATCTTTGCCAGGAGGCTGAGTTTGTACAACTTAAAAGTTTAGAAATTCTGGGTTTTATGTATAACAAT ATATTTTAATAAATGATATTTTATTGAAGTCTTAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3501285 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3501285 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10883 same embryo
	EMAGE:10887 same embryo
	EMAGE:10888 same embryo
	EMAGE:10886 same embryo
	EMAGE:10884 same embryo
	EurExpress:euxassay_003499 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source