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EMAGE:10886

Dbr1 debranching enzyme homolog 1 (S. cerevisiae) ( MGI:1931520)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10886 EMAGE:10886 EMAGE:10886 EMAGE:10886 EMAGE:10886
"Pseudo-wholemount" of euxassay_003456. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003456_01 euxassay_003456_02 euxassay_003456_03 euxassay_003456_04
EMAGE:10886 EMAGE:10886 EMAGE:10886 EMAGE:10886 EMAGE:10886
euxassay_003456_05 euxassay_003456_06 euxassay_003456_07 euxassay_003456_08 euxassay_003456_09
EMAGE:10886 EMAGE:10886 EMAGE:10886 EMAGE:10886 EMAGE:10886
euxassay_003456_10 euxassay_003456_11 euxassay_003456_12 euxassay_003456_13 euxassay_003456_14
EMAGE:10886 EMAGE:10886 EMAGE:10886 EMAGE:10886 EMAGE:10886
euxassay_003456_15 euxassay_003456_16 euxassay_003456_17 euxassay_003456_18 euxassay_003456_19
EMAGE:10886 EMAGE:10886 EMAGE:10886 EMAGE:10886 EMAGE:10886
euxassay_003456_20 euxassay_003456_21 euxassay_003456_22 euxassay_003456_23 euxassay_003456_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10886Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10886_wholemount_strong.wlz
10886_wholemount_moderate.wlz
10886_wholemount_weak.wlz
10886_wholemount_possible.wlz
10886_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10886_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
adrenal gland
moderate moderate
regionalmoderate expression: see section 10 11 17 18 19
thymus primordium
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 17 18 19 20
pancreas
moderate moderate
regionalmoderate expression: see section 09 10 11 12 15 16
thyroid gland
moderate moderate
regionalmoderate expression: see section 11 15 16
central nervous system
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
epidermis
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
vibrissa
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 21 22 23 not examined expression: see section 19 20
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 07
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 08
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 09 18
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 06 07 08 weak expression: see section 18
not examined not examined
regionalnot examined expression: see section 01 02 03 04 23 24
naris
moderate moderate
regionalmoderate expression: see section 18
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 15 16 17 18 19 20
nasal cavity respiratory epithelium
moderate moderate
regionalmoderate expression: see section 13 14 17 18
stomach
weak weak
regionalweak expression: see section 02 03 04 05 06 07 08 09 10
midgut
weak weak
regionalweak expression: see section 07 08 09 10 11 12 13 14 15 16 17
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 12 13 16 17 18
lower jaw molar
moderate moderate
regionalmoderate expression: see section 07 08 20
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 12 13 16 17 18
upper jaw molar
moderate moderate
regionalmoderate expression: see section 07 08 20
liver
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
metanephros
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 17 18 19 20 21
lung
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
tail dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 16 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6263
Entity Detected:Dbr1, debranching enzyme homolog 1 (S. cerevisiae) ( MGI:1931520)
Sequence:sense strand is shown

>T6263
CGAGCCGCGGCTCGGTTGGTGTTGGCGGGAGCCGGGTGGGCCGGCTGCCTGGTGTTCTTTGCTGTGCGAC
CCGGGCTGCAGGTGCTCGGGGGTGTTGCTGTGGAGTTCCGCGGCCCAACGTCACGATGCGGGTGGCCGTG
GCCGGCTGCTGCCACGGCGAGCTGGACAAGATCTACGAGACTCTGGCGCTGGCGGAGCGGCGCGGCTCGG
GACCCGTGGATCTCCTTCTGTGCTGCGGCGACTTCCAGGCGGTGCGCAACGAGGCCGACCTGCGCTGCAT
GGCCGTGCCGCCCAAGTACCGTCACATGCAGACCTTCTACAGATATTACTCTGGAGAGAAAAAGGCCCCG
GTTCTCACCATCTTCATTGGAGGAAACCATGAAGCCTCAAACCATTTGCAAGAGTTACCCTATGGTGGCT
GGGTAGCGCCGAATATTTATTATTTAGGCTTGGCTGGTGTTGTCAAATACCGAGGAGTAAGGATTGGTGG
CATCTCTGGCATATTTAAATCTCATGATTATAGAAAAGGTCATTTTGAGTGCCCCCCTTATAATTCATCT
ACCATAAGGAGTATATATCATGTGAGAAATATAGAGGTCTACAAATTAAAACAGTTAAAGCAGCCTGTAC
ATATATTCTTGTCTCATGATTGGCCAAGAAATATATATCATTATGGCAATAAGAAGCAGCTTCTTAAGAC
CAAATCTTTTTTCCGCCAAGAAGTGGAAAACAGCACCCTGGGGAGCCCCGCTGCCTCGGAGCTGCTGGAG
CACCTACAGCCTGCGTACTGGTTCTCCGCGCACCTGCATGTCAAGTTCGCAGCCCTGATGCAGCATCAGG
CCACAGATAAAGGCCGAGCAGGCAAAGAAACCAAGTTTTTAGCCTTGGACAAGTGCTTGCCACACAGAGA
CTTCCTTCAGGTGTTAGAGATAGAACATGACCCCAGTGCTCCTGAGTACTTAGAGTATGACGTTGAATGG
CTTACTGTTCTCAGGGCCACTGATGACCTGATTAATGTGACTGGGGGCCTGTGGAATATGCCTGAAGACA
ACGGTCTGCACACAAGGTGGGATTATAGTGCAACGGAAGAAACTATGAAGGAAGTAATGGAAAAATTGAA
CCATGACCCTAAAGTCCCCTGTAACTTCACCATGACAGCTGCTTGTTATGATCCCAGTAAGCCCCAGACA
CAAGTGAAGCTGGTTCACAGGATCAATCCACAGACGACTGAGTTCTGTGCCCAGCTTGGCATCACAGACA
TCAATGTCATGATTCAGAAGGCCAGAGAGGAAGAGCACCATCAGTGTGGTGAATATGAGCAGCAGGGTGA
CCCGGGGACTGAGGAGTCTGAAGAGGACCGGAGTGAGTACAACACGGACACGTCGGCCCTCTCCTCCATT
AATCCAGATGAAATCATGCTGGATGAAGAGGAGGAGGAGGAGGAGGAGGCTGTAAGCGCTCACAGTGACA
TGAACACACCTTCTGTGGAACCTGCTTCTGACCAGGCTTCTGACCTCTCTACAAGCTTTTCTGACGTCAG
GAACTTACCAAGTTCCATGTTTGTGTCCTCTGACGATGCATCCCGTTCTCCAGCAAGTGGAGAGGGGAAA
TGTGGTGAAACGGTGGAGTCTGGGGATGAAAAAGACTTGGCTAAGTTTCCTCTGAAGCGGCTGAGTGATG
AACACGAACCTGAACAAAGGAAGAAAATAAAGAGGAGGAACCAAGCCATCTACGCTGCAGTGGATGATGG
GGATGCTAGTGCCGAGTGAGACAGAGGCTGGACTGTGATAAATGTAGGGGTGGGATTTCCAGACCCTGTT
TTTAGGGCAGAATTTCTGATTTATTACTGGACTTTGTAATAATGAAATGATGGAATTTTGGTTATAAGCT
CTGTATTTAGATGTATGTTTATATGATTCAGATATAGACTTATTAAATTTCATATGAGATTTGATTGATG
ACCATTTTTAAGACATTCACTTCCATAGTTTTCAGCACTTAGTACACATGAATTTTGAGAAACACTGTTC
TCTAAGATGGGACTATAAAGATTTTGTAAATACATTAAATGCCACTTAATTGCTGAAATTATCCTATTGG
CTCTTGTATATTACATTTTGACTGAATTTTTATGTAACTTTGGTACCTATATTCAAGTTATCTTGGGATT
GTACAGTTTAATATTAAATGATATTATATTAATAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3501390 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3501390 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10883 same embryo
 EMAGE:10887 same embryo
 EMAGE:10888 same embryo
 EMAGE:10884 same embryo
 EMAGE:10885 same embryo
 EurExpress:euxassay_003456 same experiment
 MGI:4824239 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS