EMAGE mouse gene expression spatial database

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EMAGE:10887

Cenph centromere protein H ( MGI:1349448)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003389. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003389_01	euxassay_003389_02	euxassay_003389_03	euxassay_003389_04

euxassay_003389_05	euxassay_003389_06	euxassay_003389_07	euxassay_003389_08	euxassay_003389_09

euxassay_003389_10	euxassay_003389_11	euxassay_003389_12	euxassay_003389_13	euxassay_003389_14

euxassay_003389_15	euxassay_003389_16	euxassay_003389_17	euxassay_003389_18	euxassay_003389_19

euxassay_003389_20	euxassay_003389_21	euxassay_003389_22	euxassay_003389_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10887_wholemount_strong.wlz

10887_wholemount_moderate.wlz

10887_wholemount_weak.wlz

10887_wholemount_possible.wlz

10887_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10887_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 12 weak expression: see section 13 14 15 16

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 10 19 weak expression: see section 09 18 20

vibrissa

moderate

regional

moderate expression: see section 07 08 22

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 11 12

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 10 11 12 14 15 16 17 18 19 20 21 22 23 weak expression: see section 09

midbrain ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6267
Entity Detected:	Cenph, centromere protein H ( MGI:1349448)
Sequence:	sense strand is shown >T6267 GAACTGCTGGGTTATTTTCTGTGGCGTTCAAACCTGCGGGCCTTTGCAATGGAGGAGCAGCCGCGGGAGA GGTCTGAGGCCGGGGCTGAGGCCTGCGAAGAGAAGCGGGGTCTGTCGCAGGCCGCGGAGGAGCGTATCGA GGACCGTATCAGCCTGCTGCTCAGGCTGAGAGCTCAGACAAAACAGCAGCTCTTAGAATATAAGTCCATG ATCGACACAAATGAAGAAAAAACTCCAGAACAGATTATGCAAGAAAAACAGATCGAAGTTAAAATTGAAG AGCTGGAGAATGAAATTGAAGATGTAAAAAGTAATATTGAAATGAAAAGTCTTGCACTGAGCAGGATGAA ACTTTCAGTTGCACTTCGGGATAACATGGAGAACATGGGACCTGAGAATTGTGTGCTCACAGATGACATG AAGCACATATTAAAGCTACAAAAGTTAATAATGAAGTCCCAGGAGGAGTCTTCAGAACTGGAGAAAAAGC TGCTTGATGTCAGAAAGAAGAGACTACAACTAAAGCAAGCTTCAAGAAGTAAGCTTTTAGAAATACAAAT CGAGAAGAACAAACAGAAAGAGGATGTCGACAAGATGGAGAATTCTGAAATGATAAAGACCATGAAAAAA AAACTACAGACGGAGATAAAGATCACTACAGTTGTTCAACATACATTCCAGGGCCTTATTTTGGCGAGTA AAACCAACTGGGCAGAGGACCCTGCCCTCAGAGAGACAGTGCTGCAGCTGGAGAAGGACCTCACCACTCT ATGAGAAGAATCCCGCAGTTTAAAATTGGCTTTGTGACCAATCTGTCATTTAAATAGCCGAGTTCATGGG AAATGTAAATGGTGTTTTTGGAACCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3501244 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3501244 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10883 same embryo
	EMAGE:10888 same embryo
	EMAGE:10886 same embryo
	EMAGE:10884 same embryo
	EMAGE:10885 same embryo
	EurExpress:euxassay_003389 same experiment
	MGI:4823807 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source