EMAGE mouse gene expression spatial database

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EMAGE:10906

Ctns cystinosis, nephropathic ( MGI:1932872)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003334. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003334_01	euxassay_003334_02	euxassay_003334_03	euxassay_003334_04

euxassay_003334_05	euxassay_003334_06	euxassay_003334_07	euxassay_003334_08	euxassay_003334_09

euxassay_003334_10	euxassay_003334_11	euxassay_003334_12	euxassay_003334_13	euxassay_003334_14

euxassay_003334_15	euxassay_003334_16	euxassay_003334_17	euxassay_003334_18	euxassay_003334_19

euxassay_003334_20	euxassay_003334_21	euxassay_003334_22	euxassay_003334_23	euxassay_003334_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10906_wholemount_strong.wlz

10906_wholemount_moderate.wlz

10906_wholemount_weak.wlz

10906_wholemount_possible.wlz

10906_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10906_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5967
Entity Detected:	Ctns, cystinosis, nephropathic ( MGI:1932872)
Sequence:	sense strand is shown >T5967 CTCAGAGAGTCCTTACACTCCTAAGCGGTTTCTGGTGCGAGATTGTTGCTTTTGGTGAGGTCTGTCCACC AAGCCTGGGAAGCTGTCAGTTCTGAGAAGTCAGAGACCATGAGGAGGAATTGGCTGCTTATTTTGACCCT TTTTCTCTTGATGTTCATAGAGAAGTATGAGTCAACTGTCAGTCTCACTGCTCCTCCCACCGTGAAGCTG GAAAATGGCAGTTCAACCAACGTCGACATCACCCTTGGGCATCCACTAAATTCGACCTTGGTGATCACTT TTGAAGTCACGTTTCGCTCAAAAAATCTGACTATTGTGGAGCTTCCTGATGAAGTTATAGTGCCTCGCGG AGAGAAAAATGCCTCTTTCCAAGTGACTTCTCAAAATATTGGACAAGTGACTGTTTTTCTGCATGGAAAT CATTCCAACCAGACCTGCCCCAGGATCCGGTTCTTGGTGATACACAGCAGAATCGTTAGCATCATAAACC AGGTGATTGGCTGGATCTACTTCATGGCGTGGTCTGTCTCCTTTTACCCACAAGTGATCCAGAACTGGAG ACGGAAAAGTGTCATTGGTCTCAGCTTTGATTTCTTGGCCCTGAACTTGACGGGCTTCGTGGCCTACAGT GTTTTCAACATCGGTCTTTTGTGGGTGCCCTATATCCAGGAGGAGTTTCTCCTCAAATACCCCAATGGTG TGAACCCCGTCGACAGTAATGATGCCTTCTTCAGCTTGCATGCTGTCGCCCTCACCCTGATTGTCATCCT GCAGTGCTGCCTGTATGAGCGAGGCAACCAGCGTGTGTCATGGCCCTCCATTGGCTTCCTGGTACTCGCA TGGCTCTTTGTCCTAGTCACCATGATTGTGGCTGCAGTCGGTATCACCACATGGCTCCAGTTCCTCTTCT GCTTCTCCTACATCAAGCTCATCATCACACTGATCAAGTATTTCCCACAGGCCTATATGAACTTCTACTA CAAAAGCACCAAGGGCTGGAGCATTGGCGGTGTGCTCCTCGATTTTACAGGGGGCAGCTTTAGCCTCCTC CAGATGTTCCTCCAGTCTTACAATAATGACCAATGGACATTGATCTTCGGAGACCCAACCAAGTTCGGAC TTGGCGTCTTCACCATCTTCTTTGATGTTGTCTTCTTCATCCAGCACTTCTACTTGTACAGAAAGAAACC AGGGTACGACCAGTTGAATTAGAATCCAGAGACCCAGTGTAAACAGCCAGGGCCTGGGCCCTGGTAGGAA AGGTTGTATCCAGCAAAGGCCAGAGAAGCAGCTGGCACACAGGGCTGGAACAGTATGCCAACAAGAGCAG CATTCTCTTCCTCAAGAATCAAAAGCCTTAAGCTCAGCCTGCCTGTCTTCATCCAGGCCTCTCCTGAACC ATGGAGGACTCCCTCTCTGGAACAGACACCTGACTCTGCTTGAGACTGAAAGCTAGACTTGAGGCAGTCT CTCCCAACTTTAAAGCTCCAGCCCTCTGCTGGAGGCACCCAGCCCTCCTGCGTCTTGATATACCATCTCT CTCTCTTTAAGGCTTCAGGCAGCACACACAGGTCCAGACAGCCATCCCAGCCAGAACTGGGCATCATGTT TGCAGCTGATGACCTTGCCCCAAAGCACCAATATTTCTGGGAGCAGCTTGAAAAGCTAACCTTGCAACTG GGAGAGCCAAGGGTGCTTTGTCCCACCACAGTGTTCACAGAGACCAAGCAGCCAGGTGCTGTGGCCCATG GACTTGAAGGTGCTGATGCTGGTGGTGGAGGGGAGAGAACTGTGGGATCAAGCTTTGGGGTCCTTGCTCT CGGGGCCACTTTCCTTATGCTCATTCTATATAACGCTGCTCTAGTAACTGCAGAACAAGAGGCCCAGGCC AGTGCCTGGTAAAGTATATTTCTGATCCCTGAGGTGCCTGCCAGATTGGTCTGAAGTGGACCCGTACCTG CTGCCCTCCGGATCTTTGCACACAAAGATACCACAGTACCTTTTTTATTTTTATTTTACCTCCAAGGTAT ATTTTAAAATGGCTTTTCTTTTGCCCTCTTTCCTACCTCCACTTTCTGAGAGTCGGATGGAGCACCATCT TGAGTCCCCAGACTCCCCGTAACCTTACACCTATGGAGCAGGTACTCAGGACCAGCCTGTGCCTTAGAAG CCCATCAGTCAATCAGTAAGCTGCCCTGGATGGGAAGGGAGGCCTCCTTCCCCAGCCCCAGACTGAGACT CCAACTCCTCAGCTGTTGAGGAGCCTCAGCGCACAGGGGCTTACCTCCTATGTTGCTTGTGGTCCCACCC TGATCCCACACCTCCTTCCTTCTCATCCCATTATTCTTCCTACGCCAGGTAGGGAAGGAACCAGGGTGTG CCTTGAGTTTATCAATCTGTTTCTTTTGTTTCTTATAAGACGAATATTGAGGGATCCTTGTATTTCTTTC TTTTGTATTTTTTTCTTGTTCGGTTTCATTTTTGTGGCGTGTGTGTGTGTCCTTAATTAAAAGGTGGTTT GTTTCTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3484952 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3484952 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10903 same embryo
	EMAGE:10902 same embryo
	EMAGE:10901 same embryo
	EMAGE:10905 same embryo
	EMAGE:10904 same embryo
	EurExpress:euxassay_003334 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source