EMAGE mouse gene expression spatial database

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EMAGE:10912

Arl16 ADP-ribosylation factor-like 16 ( MGI:1917567)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006483. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006483_01	euxassay_006483_02	euxassay_006483_03	euxassay_006483_04

euxassay_006483_05	euxassay_006483_06	euxassay_006483_07	euxassay_006483_08	euxassay_006483_09

euxassay_006483_10	euxassay_006483_11	euxassay_006483_12	euxassay_006483_13	euxassay_006483_14

euxassay_006483_15	euxassay_006483_16	euxassay_006483_17	euxassay_006483_18	euxassay_006483_19

euxassay_006483_20	euxassay_006483_21	euxassay_006483_22	euxassay_006483_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10912_wholemount_strong.wlz

10912_wholemount_moderate.wlz

10912_wholemount_weak.wlz

10912_wholemount_possible.wlz

10912_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10912_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6782
Entity Detected:	Arl16, ADP-ribosylation factor-like 16 ( MGI:1917567)
Sequence:	sense strand is shown >T6782 GCGTTATCCGAGGAAGGGATGTGTCTCCTGCTTGGAGCTGCTGGGGTCGGGAAGACCCTGCTGGTGAAAC GCCTTCAGAAGCTGAGCTCCGGAGATGGCAAAGGCGACCTGGGAGAGCCCCCACCGACGCGGCCCACGGT GGGCACCAACCTTACAGACATTGTGGCCCACAGGAAGATCACCATCCGAGAGCTCGGGGGATGCATGAGC CCCATCTGGTCCAGTTACTATGGAAACTGCCATTCGCTCTTGGTGAGTTAATACTTCAAGCTTCTTTGGA GGTCAGTTTTGTCTGACTCCCTGAACCATCAAGTATTGGCCCTGCTTTTCATGGGATCGGGTTTGGGGGT CTTTGATGACTGTGCATTGGTCTGATTTCCTGAGCAGGGCTCAGATTCTAGGAGGCTTGTTGAAGGCACA GCATGTTCTATGCCTTGATACCTGAATACGACCAACACTTTACATCCCTCTTGTTGGTCCTATCTGTAGA AGGAAGGGGCATCAAATTCTGGGATGAAGAGCTGATGGCCACAGTAGACTTAATTTTACTTAAAGACACC AGTGACAGTCATCCTCGTACAACTCCCCCTTGGCTATTTCCAAAACCTGTTGTTTAAAAGCCAATCTGGG GCTGGATATGTAGCTCAGGAGAGTGCTTGCCCTAGCTACACTTGAAGCCCTAGGTTCAAACTCCTGCATC ATAAATTTAAATCCACTTAAAAAGTTGTCTGTGTGCTTTTGTGAAATGACCAGACAGGGCTCTGGTCAGG ACTATCTTCTGTAGCATGTTTTCCTTTTCTCCCTAGTTCATGATGGATGCTTCTAACCCCACCCAGCTCT CTGCATCCTGCATGCAGCTCCTGGGTCTCCTTTCTGCAGAAGAACTTGAAAAAGCCTCCGTCCTCATACT CTTCAATAAAATGTATGGGTCTAAGGGCTGGGCAGGATGAAGGGCAAGGTATCCCAAGGGGTTGCTCTAC TTGAGTACAGCCAAGACCATAGGGTCTGCAGCGGGGAGGTGCAGCTGTGGGTAGAACAACTTTGTGGCGA GCACCACTTTGACGAAGTGCTTCTTCAACTACAGTGACCTACCCTGTTACATGACCATGGAGGAAATGAA GTCATTGATGAGGCTTCCAGACATCATTGCTTGTGCCAAGCAAAACATCACCGCAGTAGAAATAAGTGCC AGGAATGGCACTGGCTTGGCAACAGTATTGCTCTGGCTCCAGAATCAACACAGGCACAGCAGTTGACAGC AAGATGGAGAAGTATGGCTGACCCCACCCAGTGCCTAGGAGGCAAATGCATGAGAGCCTGAGGTGTTTGA TAGGAGCAGGCCACCCAGTGTATCTGCAAGTGATGGGGGCTGTGCTGTGACCAGTAAACAAGCATACACT TACAAACACCACTGTCATCTCACTTCTGAACAATGGTCCCAAGACAGAACAAACTGCAGGTGTCTTCACG TCTCCCACCAAGAAGTTGGACCCACTGAGGGCTTTGGACTGCACGGAGCCCAGGTCATTTTTATAGTACA GACTCTGCCTTTCTGAGTTCCTATATCCTGACCATGTTGCCCAGAAAAAAATATAAAGTTAATGAAGAAT TGGGAAGTGGGCCGGGCATGGTGGCACATGCCTTTAATCCCAGTACTCGGGAGGCAGAGGCAGGTGGATT TCAGAGTTCGAGGCCAGCCTCGTCTACAAAGTGAGTTCCAGGACAGCCAGAGCTACACAGAGAAACCCTG TCTCGAAAACCAAAAAAAAATTTGGGAAGTTGGGGAGGGGTGAGATGGCTCAGTGGGTAAGAGCACTGGC TTCTCTTCAGAAGATCCTGAATTCAAATCCCAGCAACCGCATGGTGGCCCACAGCCCTCTGTAATGAGAG CTGACTCACTCTTCTGGAGTGTCTAAAGACAGCTACAGTAGTGAGCAGAGGTTGAGTTCAATTCCCAGCA ACCACATGATGGCTCACAATCATCTATACAGCTACAGTGTACTCATATACATAAAATATAATTTAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3708096 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3708096 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10913 same embryo
	EMAGE:10911 same embryo
	EurExpress:euxassay_006483 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source