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EMAGE:10932

Tmsb4x thymosin, beta 4, X chromosome ( MGI:99510)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10932 EMAGE:10932 EMAGE:10932 EMAGE:10932 EMAGE:10932
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EMAGE:10932 EMAGE:10932 EMAGE:10932 EMAGE:10932 EMAGE:10932
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EMAGE:10932 EMAGE:10932 EMAGE:10932 EMAGE:10932 EMAGE:10932
euxassay_003553_11 euxassay_003553_12 euxassay_003553_13 euxassay_003553_14 euxassay_003553_15
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euxassay_003553_21 euxassay_003553_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10932Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10932_wholemount_strong.wlz
10932_wholemount_moderate.wlz
10932_wholemount_weak.wlz
10932_wholemount_possible.wlz
10932_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10932_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6268
Entity Detected:Tmsb4x, thymosin, beta 4, X chromosome ( MGI:99510)
Sequence:sense strand is shown

>T6268
GACTCTCCTCGTTCGCGCAGCTCGCTCGGCTCCTTCCAGCAACCATGTCTGACAAACCCGATATGGCTGA
GATCGAGAAATTCGATAAGTCGAAGTTGAAGAAAACAGAAACGCAAGAGAAAAATCCTCTGCCTTCAAAA
GAAACAATTGAACAAGAGAAGCAAGCTGGCGAATCGTAATGAGGCGAGCGCCGCCAATATGCACTGTACA
TTCCACGAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAACTTTGTAAGATGCAAAGAGGTTGGA
TCAAGTTTAAATGACTGTGCTGCCCCTTTCACATCAAAGAATCAGAACTACTGAGCAGGAAGGCCTCCCC
TGCCTCTCCCACCCATCTGATGGTCTGGCTAGCAGAGAGGGAAAAGAACTTGCATGTTGGTGAAGGAAAA
AGCTGGGTGGGAGATGATGAAATAGAGAGGAAAATTCAAGATGGTCAAAGATGTCCTGCAGGATGTAAAA
TGCAGTTTAATCAGAGTGCCATTTTTTTTTTGTTCAAACAATTTTAATTATTGGAATGCACAATTTTTTT
AATATGCAAATAAAGTTTTAAAACCTGAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3501378 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3501378 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10931 same embryo
 EurExpress:euxassay_003553 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS