EMAGE mouse gene expression spatial database

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EMAGE:10943

Enoph1 enolase-phosphatase 1 ( MGI:1915120)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003092. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003092_01	euxassay_003092_02	euxassay_003092_03	euxassay_003092_04

euxassay_003092_05	euxassay_003092_06	euxassay_003092_07	euxassay_003092_08	euxassay_003092_09

euxassay_003092_10	euxassay_003092_11	euxassay_003092_12	euxassay_003092_13	euxassay_003092_14

euxassay_003092_15	euxassay_003092_16	euxassay_003092_17	euxassay_003092_18	euxassay_003092_19

euxassay_003092_20	euxassay_003092_21	euxassay_003092_22	euxassay_003092_23	euxassay_003092_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10943_wholemount_strong.wlz

10943_wholemount_moderate.wlz

10943_wholemount_weak.wlz

10943_wholemount_possible.wlz

10943_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10943_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 weak expression: see section 10 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4699
Entity Detected:	Enoph1, enolase-phosphatase 1 ( MGI:1915120)
Sequence:	sense strand is shown >T4699 CCACGCGTCCGGTCCCAGCTCTGTCTCTGAGCCCCTGGTCGTTCGCTGTCGCCAGCGCCTCGGTTGTTGC TGGGCCCTCGGGAGCTCAAGACGCGCAGGCGGTGTTGCCCTCCTTAACCAAGGCCGAGCCCGTGGTAGCA TCCGGCAGGGAAATGGTCGTGGTTTCCGTGCCCGCCGAAGTCACCGTAATCCTGTTAGATATCGAAGGTA CCACAACCCCGATTGCTTTCGTGAAGGATGTTTTATTTCCGTACATCAAAGAAAATGTCAAGGAGTACCT GCAGACCCACTGGGAGGAGGAGGAGTGTCAGCAGGACATCAGCCTGCTGAGGAAACAGGCCGAGGAAGAT GCACATCTGGACGGGGCTGTTCTGATACCCGTGGCTTCTGGCAGTGATCTGCAGCAGATGATCCAGGCTG TGGTGGACAACGTGTACTGGCAGATGTCCCATGACCGAAAGACCACAGCGCTCAAACAGCTGCAAGGCCA CATGTGGAAGGCAGCTTTCACGGCTGGCCGCATGAAGGCAGAGTTCTTTGCAGATGTGGTTCCTGCAGTC AGAAGGTGGAGAGAGGCTGGGATGAAGGTATACATCTATTCTTCAGGGAGCGTGGAGGCACAGAAGCTGC TCTTCGGCCATTCCACAGAGGGAGACATTCTTGAGCTCATCGACGGTCACTTTGACACCAAGATTGGACA CAAAGTGGACAGTGAGAGTTACCGGAAGATTGCTGATAGCATCGGTTGTTCAACCAACAACATCCTGTTT CTCACGGATGTCACCGTAGAGGCCAGTGCTGCTGAGGAGGCGGACGTGCACGTGGCTGTGGTGGTGCGAC CTGGCAACGCGGGGCTCACTGACGACGAGAAGACATACTACAACCTCATCACGTCCTTCAGCGAGCTCTA CCTGCCGTCCACCTAGAGCCAGGTCTGAGGAAGAATTGCCGTCCCCTAGTGTCTAGCTTTATTCTAACTG TAAAAGTAACGTACTTATGTGTGCACACAGACATGTGCAAGGGTATATACATGGACATGTGCTCAGATTA GTTTCCATGGGCACATAATGTGAGAAAAGTTGTCCGTTCCATGAAGACCAAATTCATGTAAAGACACTTT ATGTGTAGACCATAAATCTGTCTTACCAAGCCCGGGGTGTATTTGAAACTGCAAAATACTAATCAAATTT GTGATGTTTATCAAATTGTTTACCAAGTGGAAAGCTAGTTCTGAGAAATTATTATCCAGAGCCTGTGTTA TTTTAAAGACTGGACTTGCTTCAAAGGTACCCATACGAGGAGCCCACCGTCCCTGTCCCTCGAGGTCACG TCCCACCGCTGCACTAAGGACTCACGGCCCAGCGTCCACAAGACTGAGGCCCTGTCCCTGCTCAGAGAAC AGATCCGGAGCAGGTGGTCTGGTCAGTCAGGATGGAACTGCCTCTGAGCAGTGCTCGGAACAGCTGAGGC CGTGGCGGGCTCCACTAACCCCCAGTGTTATCGTGTTTTATAGCTGCCTTTCTGTGTCTTACACAGTTTG AGAGTATATGTTAATGACTATTTTTTTTAAAAATGTTCTATTGAAACCAGTGTAACAGCACAAAGAGCTC TAATGTGTAACACTTCCTCTGAAATGGATATAAAGATGTAAATTTTATAACAGCACTGTTTATTCTGATG CATTCTGATGGGTCAGTGTCGTGTCATTAATTAATAAAGGCATTTTCTTCTCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5030617 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5030617 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4824555 same experiment
	EurExpress:euxassay_003092 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source