EMAGE mouse gene expression spatial database

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EMAGE:10959

Prpf6 PRP6 pre-mRNA splicing factor 6 homolog (yeast) ( MGI:1922946)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003459. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003459_01	euxassay_003459_02	euxassay_003459_03	euxassay_003459_04

euxassay_003459_05	euxassay_003459_06	euxassay_003459_07	euxassay_003459_08	euxassay_003459_09

euxassay_003459_10	euxassay_003459_11	euxassay_003459_12	euxassay_003459_13	euxassay_003459_14

euxassay_003459_15	euxassay_003459_16	euxassay_003459_17	euxassay_003459_18	euxassay_003459_19

euxassay_003459_20	euxassay_003459_21	euxassay_003459_22	euxassay_003459_23	euxassay_003459_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10959_wholemount_strong.wlz

10959_wholemount_moderate.wlz

10959_wholemount_weak.wlz

10959_wholemount_possible.wlz

10959_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10959_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6685
Entity Detected:	Prpf6, PRP6 pre-mRNA splicing factor 6 homolog (yeast) ( MGI:1922946)
Sequence:	sense strand is shown >T6685 GGAGGACTCTGAGTCTTCTGTCGTATACTTGCTCCCCTGCTTTTCTGTGTTTCGGTCAATAACACCGCCA TGAACAAGAAGAAGAAGCCGTTCCTGGGCATGCCTGCGCCGCTGGGCTACGTACCCGGGCTGGGCAGGGG TGCCACTGGCTTCACCACCCGATCAGACATCGGGCCTGCTCGGGATGCAAATGATCCTGTGGATGATCGC CATGCCCCACCAGGGAAGAGAACTGTGGGGGACCAGATGAAGAAAAATCAGGCGGCTGATGATGATGATG AAGATCTGAATGATACCAACTACGATGAGTTTAATGGCTATGCTGGGAGCCTCTTCTCAAGTGGGCCCTA TGAAAAAGATGATGAGGAAGCAGATGCTATTTATGCAGCTCTGGATAAAAGGATGGATGAGAGAAGAAAA GAGAGAAGAGAGCAAAGGGAGAAAGAAGAGATTGAGAAATACCGAATGGAACGTCCCAAAATCCAGCAGC AATTTTCAGACCTTAAGAGGAAGCTGGCAGAAGTCACAGAAGAAGAGTGGCTGAGCATCCCAGAAGTTGG CGATGCCAGAAACAAACGTCAACGGAACCCACGTTATGAGAAGCTGACTCCTGTTCCTGATAGTTTCTTT GCCAAACATTTACAGACTGGAGAGAACCATACTTCTGTGGATCCCCGACAGACTCAATTTGGAGGTCTGA ACACACCCTACCCGGGAGGCCTGAACACTCCATACCCAGGTGGAATGACACCTGGATTGATGACACCTGG TACAGGGGAGCTGGACATGAGGAAGATTGGCCAGGCAAGGAATACCCTGATGGACATGAGGCTTAGCCAG GTATCTGACTCAGTGAGTGGGCAGACTGTTGTGGACCCCAAAGGCTACCTGACAGATCTAAATTCCATGA TCCCAACCCATGGCGGGGACATAAATGACATCAAGAAAGCACGACTACTTCTCAAATCTGTTCGAGAAAC AAACCCTCATCACCCACCAGCTTGGATTGCATCAGCTCGCCTGGAAGAAGTCACTGGAAAGTTACAAGTA GCTCGAAACCTTATTATGAAAGGGACAGAGATGTGCCCCAAGAGTGAAGATGTCTGGCTAGAAGCAGCTA GGTTACAGCCTGGGGACACAGCCAAGGCTGTGGTGGCACAAGCTGTCCGTCATCTCCCGCAGTCTGTTAG GATTTACATCAGAGCAGCAGAGCTAGAAACAGACATTCGAGCAAAGAAGCGTGTTCTTCGGAAAGCACTT GAGCATGTTCCAAACTCTGTTCGATTGTGGAAAGCAGCTGTTGAGCTTGAAGAGCCTGAAGATGCTAGAA TCATGCTGAGCCGAGCAGTAGAGTGCTGTCCCACCAGTGTAGAGCTATGGCTTGCTTTGGCAAGACTAGA AACTTATGAAAATGCTCGCAAGGTCTTAAATAAAGCACGTGAGAACATTCCTACAGATCGGCACATCTGG ATCACAGCTGCCAAACTGGAGGAGGCCAATGGGAACACTCAGATGGTGGAAAAGATCATTGACCGAGCCA TCACCTCCCTACGGGCCAATGGTGTAGAGATCAACCGCGAACAGTGGATCCAGGATGCTGAGGAGTGTGA CAGGGCAGGGAGTGTGGCCACGTGCCAGGCTGTCATGCGTGCTGTAATCGGTATTGGGATTGAGGAGGAA GATCGCAAACACACCTGGATGGAAGACGCTGACAGTTGTGTGGCCCACAATGCCCTGGAGTGTGCACGAG CCATATATGCCTATGCCCTACAAGTCTTCCCCAGCAAGAAAAGTGTGTGGTTACGAGCTGCCTACTTTGA GAAGAATCATGGTACCAGGGAGTCTCTGGAAGCACTCTTGCAGAGGGCTGTAGCCCACTGCCCCAAGGCA GAGGTGCTATGGCTCATGGGTGCCAAGTCAAAGTGGCTGGCAGGGGATGTACCTGCAGCAAGGAGCATCC TGGCCCTGGCCTTTCAGGCCAACCCCAACAGCGAAGAGATCTGGTTGGCGGCTGTGAAGCTGGAGTCAGA GAACAATGAATACGAGCGAGCCCGGAGGCTACTGGCCAAGGCTCGGAGCAGCGCACCCACTGCCCGTGTA TTCATGAAGTCTGTGAAGCTGGAGTGGGTTCTGGGGAACATCTCTGCGGCGCAGGAGCTGTGTGAGGAGG CTCTGAGGCACTATGAAGATTTCCCCAAGCTGTGGATGATGAAGGGGCAGATTGAGGAGCAGGGAGAACT GATGGAGAAGGCCCGGGAGGCCTACAACCAGGGGCTGAAGAAATGTCCTCATTCCACACCGCTTTGGCTC TTGCTTTCCCGACTAGAGGAAAAGATTGGGCAGCTGACACGTGCTCGGGCCATTTTAGAGAAATCTCGTC TGAAAAACCCAAAGAACCCTGGGCTATGGTTGGAGTCAGTGCGGCTGGAGTACCGGGCAGGGCTGAAGAA CATTGCCAATACCCTCATGGCCAAGGCGCTGCAGGAGTGCCCTAACTCTGGTATCCTGTGGTCTGAAGCT GTCTTCCTTGAGGCAAGGCCCCAGAGAAAGACCAAGAGTGTGGATGCCCTGAAGAAGTGTGAACATGACC CCCATGTGCTTTTAGCTGTGGCTAAGTTGTTCTGGAGTGAAAGAAAGATCACCAAGGCTCGGGAGTGGTT CCATCGCACTGTGAAGATTGACTCAGATCTGGGGGATGCCTGGGCCTTCTTCTACAAATTTGAACTGCAG CATGGAACTGAGGAGCAACAGGAGGAGGTGAGGAAACGCTGTGAGAATGCAGAGCCCCGACATGGAGAGC TGTGGTGTGCTGTGTCCAAGGATATCACCAACTGGCAGAGGAAGATTGGGGAAATCCTAGTCCTGGTGGC CGCCCGCATCAAAAATACCTTCTGATAGGAGGGGTGACAATAGGAGCAACACATGGACAATGGGCACAAA CCCTACACTGTCTTTTACTCATTAAAGGTTTTTATAGAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3602158 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3602158 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10958 same embryo
	EMAGE:10961 same embryo
	EMAGE:10960 same embryo
	EMAGE:10962 same embryo
	EurExpress:euxassay_003459 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source