EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10962

Sftpd surfactant associated protein D ( MGI:109515)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003496. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003496_01	euxassay_003496_02	euxassay_003496_03	euxassay_003496_04

euxassay_003496_05	euxassay_003496_06	euxassay_003496_07	euxassay_003496_08	euxassay_003496_09

euxassay_003496_10	euxassay_003496_11	euxassay_003496_12	euxassay_003496_13	euxassay_003496_14

euxassay_003496_15	euxassay_003496_16	euxassay_003496_17	euxassay_003496_18	euxassay_003496_19

euxassay_003496_20	euxassay_003496_21	euxassay_003496_22	euxassay_003496_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10962_wholemount_strong.wlz

10962_wholemount_moderate.wlz

10962_wholemount_weak.wlz

10962_wholemount_possible.wlz

10962_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10962_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 16 17 18 19 20 21 22 23

otic capsule

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 17 18 19 20 21 22 23

viscerocranium

weak

regional

Expression in the turbinate bone.

clavicle

weak

regional

weak expression: see section 09 10 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6684
Entity Detected:	Sftpd, surfactant associated protein D ( MGI:109515)
Sequence:	sense strand is shown >T6684 CCTGGGGCCAACAAGGAAGCAATCTGACATGCTGCCCTTTCTCTCCATGCTTGTCTTGCTTGTACAGCCC CTGGGAAATCTGGGAGCAGAAATGAAGAGCCTCTCGCAGAGATCAGTACCCAACACCTGCACCCTAGTCA TGTGTAGCCCAACAGAGAATGGCCTGCCTGGTCGTGATGGACGGGATGGGAGAGAAGGTCCACGGGGTGA GAAGGGTGATCCAGGTTTGCCAGGACCTATGGGGCTCTCAGGGTTGCAGGGCCCTACAGGTCCAGTTGGA CCCAAAGGAGAGAATGGCTCTGCTGGCGAACCTGGACCAAAGGGAGAACGTGGACTAAGTGGACCTCCAG GACTTCCAGGTATTCCTGGTCCAGCTGGGAAAGAAGGTCCCTCTGGGAAGCAGGGGAACATAGGACCTCA AGGCAAACCAGGTCCTAAAGGAGAGGCTGGGCCCAAAGGAGAAGTAGGTGCTCCTGGCATGCAAGGATCT ACAGGGGCAAAAGGCTCCACAGGCCCCAAGGGAGAAAGAGGTGCCCCTGGTGTGCAAGGAGCCCCAGGGA ATGCTGGAGCAGCAGGACCTGCCGGACCTGCCGGTCCACAGGGAGCTCCAGGTTCCAGGGGGCCCCCAGG ACTCAAGGGGGACAGAGGTGTTCCTGGAGACAGAGGAATCAAAGGTGAAAGCGGGCTTCCAGACAGTGCT GCTCTGAGGCAGCAGATGGAGGCCTTAAAAGGAAAACTACAGCGTCTAGAGGTTGCCTTCTCCCACTATC AGAAAGCTGCATTGTTCCCTGATGGCCGAAGTGTTGGAGACAAGATCTTCAGGACAGCAGACTCTGAAAA GCCTTTTGAGGATGCCCAGGAGATGTGCAAACAGGCTGGAGGACAGCTGGCCTCCCCACGTTCTGCTACT GAGAATGCTGCCATACAGCAACTCATCACAGCCCACAACAAGGCTGCTTTCCTGAGTATGACAGATGTGG GCACAGAGGGCAAGTTCACTTACCCCACAGGAGAGCCCCTGGTCTATTCTAATTGGGCTCCAGGGGAGCC CAACAACAATGGTGGAGCAGAGAACTGTGTGGAGATCTTCACCAATGGGCAATGGAATGATAAGGCTTGT GGAGAGCAGCGCCTTGTTATCTGTGAGTTCTGAGCCACTGGAGGTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3601620 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3601620 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10958 same embryo
	EMAGE:10959 same embryo
	EMAGE:10961 same embryo
	EMAGE:10960 same embryo
	EurExpress:euxassay_003496 same experiment
	MGI:4828017 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source