EMAGE mouse gene expression spatial database

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EMAGE:10965

Snrpb2 U2 small nuclear ribonucleoprotein B ( MGI:104805)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003430. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003430_01	euxassay_003430_02	euxassay_003430_03	euxassay_003430_04

euxassay_003430_05	euxassay_003430_06	euxassay_003430_07	euxassay_003430_08	euxassay_003430_09

euxassay_003430_10	euxassay_003430_11	euxassay_003430_12	euxassay_003430_13	euxassay_003430_14

euxassay_003430_15	euxassay_003430_16	euxassay_003430_17	euxassay_003430_18	euxassay_003430_19

euxassay_003430_20	euxassay_003430_21	euxassay_003430_22	euxassay_003430_23	euxassay_003430_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10965_wholemount_strong.wlz

10965_wholemount_moderate.wlz

10965_wholemount_weak.wlz

10965_wholemount_possible.wlz

10965_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10965_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 08 09 10 11 17 18 19 20 21

vibrissa

moderate

regional

moderate expression: see section 07 08 09 10 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5951
Entity Detected:	Snrpb2, U2 small nuclear ribonucleoprotein B ( MGI:104805)
Sequence:	sense strand is shown >T5951 CGGGGAAGCTCTTCCGGTATTAGTTTCAAGCGGACCGCGCCGGCTTCAGGCTATTGGAAGGGTTCTACCC AAACATGGATATCAGACCAAATCACACGATTTATATCAACAACATGAATGACAAAATTAAAAAAGAAGAA TTGAAGAGATCCCTGTATGCCCTTTTTTCTCAGTTTGGACACGTGGTAGATATTGTGGCTTTAAAGACCA TGAAGATGAGGGGACAGGCTTTTGTTATATTTAAGGAACTGGGTTCATCCACAAATGCTTTGAGACAGTT ACAAGGATTTCCATTTTATGGTAAACCAATGAGAATTCAGTACGCAAAAACAGATTCTGATATAATATCT AAAATGCGTGGTACTTTCGCTGACAAGGAAAAGAAAAAGGAAAAGAAGAAAGCTAAAACCATGGAACAGG CTGCAGCTGCTGCAAACAAGAAGCCTGGTCAGGGAACACCAAATGCAGCTAATACCCAAGGCACTGCAGC GCCAAATCCTCAGGTCCCTGATTATCCTCCAAATTATATCCTATTTCTTAATAACTTACCAGAGGAGACA AATGAGATGATGTTATCCATGCTGTTCAACCAGTTCCCTGGATTCAAGGAAGTTCGCTTGGTACCGGGGA GACATGACATTGCATTTGTAGAATTTGAGAATGATGGTCAGGCTGGAGCTGCCAGAGATGCTCTGCAGGG GTTTAAGATTACACCGTCCCATGCCATGAAGATCACCTATGCCAAGAAGTAACATGTGATGCCAATGTGG AAGGACTTGGTTATTTATAGTGTTTGTTTTGATCATATTCGGTCAAGTCATTTTAAATGGTTGGAAGTGA AGGTGAAGTTTTGGGAGAAGAGTTGTCTAATTTTTTTTAGTTTTGCTGAACTATGAAATACGGAGCCTTA ATTTTGTACAATAAACTTTTATTTGTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3483683 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3483683 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10966 same embryo
	EMAGE:10964 same embryo
	EMAGE:10968 same embryo
	EMAGE:10963 same embryo
	EMAGE:10967 same embryo
	EurExpress:euxassay_003430 same experiment
	MGI:4828355 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source