EMAGE mouse gene expression spatial database

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EMAGE:10981

Mal myelin and lymphocyte protein, T-cell differentiation protein ( MGI:892970)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003604. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003604_01	euxassay_003604_02	euxassay_003604_03	euxassay_003604_04

euxassay_003604_05	euxassay_003604_06	euxassay_003604_07	euxassay_003604_08	euxassay_003604_09

euxassay_003604_10	euxassay_003604_11	euxassay_003604_12	euxassay_003604_13	euxassay_003604_14

euxassay_003604_15	euxassay_003604_16	euxassay_003604_17	euxassay_003604_18	euxassay_003604_19

euxassay_003604_20	euxassay_003604_21	euxassay_003604_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10981_wholemount_strong.wlz

10981_wholemount_moderate.wlz

10981_wholemount_weak.wlz

10981_wholemount_possible.wlz

10981_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10981_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

peripheral nervous system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

not examined

spotted

not examined expression: see section 17 18

kidney calyx

strong

regional

strong expression: see section 07 08 09 14 15 16 17 18

kidney pelvis

strong

regional

strong expression: see section 16

lung

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6613
Entity Detected:	Mal, myelin and lymphocyte protein, T-cell differentiation protein ( MGI:892970)
Sequence:	sense strand is shown >T6613 GTCAGTGCGGAGCCGGAGGCGAGCGTGTCCAGTCCCAAGGCCGACGCCAGCTCGCAGGCATGGCTCCGGC AGCGGCTTCGGGTGGCAGCACCCTGCCCAGTGGCTTCTCGGTCTTCACCACCTTCCCTGACTTGCTCTTC GTTTGTGAGTTTGTCTTTGGAGGCCTGGTGTGGATCCTGATTGCCTCCTCCCTGGTACCCTTGCCCCTGG CCCAGGGCTGGGTGATGTTTGTGTCTGTGTTCTGCTTTGTGGCCACCACTTCCCTGATGATCTTGTACAT AATTGGTACTCATGGCGGTGAGACTTCCTGGATCACACTGGATGCAGCCTACCACTGTGTGGCTGCCCTA TTTTACCTCAGTGCCTCAGTTCTGGAAGCCCTGGCCACCATCTCAATGTTTGATGGCTTTACTTACAAGC ATTACCATGAAAACATCGCCGCAGTGGTGTTTGCCTACGTGGTCACTCTGATCTACGTGGTCCATGCTGT GTTTTCCTTAATCAGATGGAAGTCTTCATAGGACAGCAGATCGGGAGCTGAGACCCAGATGCAATTAACT GGTCAGCCCATCTTCCCCATTAACTTCCTAGAACACAGACTGGATGGTGGAGAAAAGAAAACAAGCCAAA AAGAAAACAAAACAAACACAAAAACAAAAGGAAGCCATATTCAAGCCATATTCGCGTCTCTTGGGTGTTA TGTTTACCTTCTGTCAAGGTTTAGGGCTTGCTATATTTAACTTCTAGCTAAGGGAGGAAGGAGTTGTCTT GGAAGGGGACCTTTTTGCCCTTGACCGGGACAGTTGGTGGGAACTGGGAACCTTGATCTGAAGAAATGAC GATTTACACTTGACCCTTGAAGTATGTCCTAAGAATTTGCCTCTTGGAATTTTTTAAGGGCTCTTTTGAG CACTCATCCCCTGGCATGTCTTAGTCTTTGTGAATAGTCTGGGTGTCACAGGCACTGAAATTCACCCAAC AGAGGTCTCCGGACGTTCACAGTGGAAGATGAGCTCCTTTGAAATGCTTCATTAAATACTTTTGTGTCTC ATGCTTCAGGTGGGGAACTGGAGGGATGTCTTTCATTCTTGAGGACCAGGGACACCTTCCTGTTCAACTT TCTTCCTGCAGAGTCCTTTGATTAACCTGATTCTCAAGGGACAGTTCAGATGTGTGTGCTGGGCCAGTCT TTCCCAAAGTGACTCACTGAGGACGCCACCCTCACAACAGTGTCCACCAGGATAGCGTCTCCCTTGTAGA CACATCCCGTAGAGCTCTGCCTTTGTCTTTCAAGGCACAACAGTCTGCTGAATTAGAGTCTTCTCAAGCT CTGAGGAAACTAGGTGTGGGCATTCTTTCTATTCTTAAGGCTGATCTAAAATGTCCGGGTGTCTGCAGTC TCTTGATGTGTTTGGACATGTTCTCAGACCACTCCTTGTGGGAGAAAGGGACCTGACTCTAGAGCAAAGT GAGAGGCAATGGCTATCTCTGGCTGCCACACTCCAGGTGAGGGATGTCTGACCTGAAAGAAGAAAGTATA ATTAGGAAGGACATGGAGAATCTGCAAAGTCAGACGTTCTGCTAGGAGATCTCACTTTGTCCCCACACTT TAAAGAACTGAGTGACATCCTAGAGATGGAGTCCCAGATGCAGGGATGTCCTGCCAGCCACCAGGAGATT CAGGATTCTAGCCTCAGTCAGCTTTCACTAGCTGATGTTTTATTCTCCAACAAAGTAGGAGATTGAGACC TACAACTAAATTAGATTTGGGGATTTTTCTATGTTGGTTCTCAGGATGGAACCCAGGGGGTTTGCATGCT GGGTAAGCACTCTTCTACCAGATCCCATCATCAGCCCCAAATTGGATCTCACTGGCACATTCAGAAGGAC TTTGGTTATCCCAGCCATACTGGTATCTGAATTCACCATTTGCCCACTTAACACAGCCAAAATTTCATAC TAAAACACAAGCAAAAGATTAGGGGGAGTTATTTTTAAAAATTAAAATATAAACTAAGAGATTATACAAA GTTTATTATAATGTTTAACAAAATCTGTAACTGAGTGCTGACTGGGGTAGTCTTATACTTTCAACAAAGC TGAACCCTAACATTACACTAACCAGCAGCTCAACACGAGTGAGCATCACTTTTAGACAAGAAGAAGAAAG AGATGAAGGAAGGAAGAAAAGAGGCAAGGAAGGGTGACTAAGAGGAATTGCCCAAGGTTTCTCAATGTTT CAGATTTTTAATCATTAAAAGAAACTTTCATACAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3597561 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3597561 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10982 same embryo
	EMAGE:10984 same embryo
	EMAGE:10983 same embryo
	EurExpress:euxassay_003604 same experiment
	MGI:4826066 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source