EMAGE mouse gene expression spatial database

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EMAGE:10983

Ttl tubulin tyrosine ligase ( MGI:1916987)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003613. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003613_01	euxassay_003613_02	euxassay_003613_03	euxassay_003613_04

euxassay_003613_05	euxassay_003613_06	euxassay_003613_07	euxassay_003613_08	euxassay_003613_09

euxassay_003613_10	euxassay_003613_11	euxassay_003613_12	euxassay_003613_13	euxassay_003613_14

euxassay_003613_15	euxassay_003613_16	euxassay_003613_17	euxassay_003613_18	euxassay_003613_19

euxassay_003613_20	euxassay_003613_21	euxassay_003613_22	euxassay_003613_23	euxassay_003613_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10983_wholemount_strong.wlz

10983_wholemount_moderate.wlz

10983_wholemount_weak.wlz

10983_wholemount_possible.wlz

10983_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10983_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 09 15 16 weak expression: see section 12 13

pons mantle layer

moderate

regional

moderate expression: see section 07 08 17

facial vii ganglion

weak

regional

weak expression: see section 04 19 21

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 18

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

ventral grey horn

moderate

regional

moderate expression: see section 11 12 15 16 weak expression: see section 14

cervico-thoracic ganglion

weak

regional

weak expression: see section 11 16

cervical ganglion

moderate

regional

moderate expression: see section 09 weak expression: see section 17

thoracic ganglion

weak

regional

weak expression: see section 12 15

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 16 17 18 weak expression: see section 11 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6615
Entity Detected:	Ttl, tubulin tyrosine ligase ( MGI:1916987)
Sequence:	sense strand is shown >T6615 CGGACGCGTGGGCGGACGCGTGGGCGGACGCGTGGGCGGACGCGTGGGGGCGCGGGCGCCCAGGGCAGAC ACTAGGGTAATCCGGAGGCGCGGCCCTGGAATTAGACCGACAGCGGGGCTGAGGCTGAGGCTGGGGCAGT GTGCACGCGGGGCCGCCGTGCCCTGGACTCGTGTAGCGGCTGCGGGGCGCGCTGCCCTTCGCCATGTACA CCTTCGTGGTGCGCGACGAGAATAGCAGCGTCTACGCCGAGGTCTCCCGGCTGCTGCTGGCCACCGGCTA CTGGAAGAGGCTACGGCGAGACAACCCCAGGTTCAACCTGATGCTGGGGGAGAGGAACCGACTGCCCTTC GGGAGACTAGGTCACGAGCCTGGGCTGGCACAGCTGGTGAATTACTACCGGGGGGCGGACAAACTGTGCC GCAAAGCTTCCTTAGTAAAGCTAGTCAAGACCAGTCCGGAACTGTCTGAGTCCTGCTCATGGTTCCCCGA GTCGTATGTGATTTATCCAACTAACCTCAAGACGCCAGTCGCTCCGGCGCAGAATGGCATCCAACTTCCG GTCAGTAACTCCAGGACAGATGAGAGAGAATTCTTCTTGGCTTCTTATAACAGAAAGAAAGAGGACGGGG AGGGCAACGTTTGGATTGCAAAGTCATCAGCTGGTGCCAAAGGTGAGGGCATCCTCATCTCCTCAGAGGC ATCGGAGCTTCTGGATTTCATCGACAGCCAGGGCCAAGTGCACGTGATCCAGAAATACCTCGAGCGCCCT CTGCTCCTCGAGCCCGGCCACCGCAAGTTTGACATTCGAAGCTGGGTCCTGGTTGACCATCAGTATAATA TCTACCTCTATAGAGAGGGTGTGCTTCGAACTGCTTCAGAACCATATCATGTTGATAATTTCCAAGATAA GACCTGCCATCTGACCAATCACTGCATTCAGAAAGAGTACTCAAAGAACTATGGGAAATACGAAGAAGGG AATGAAATGTTCTTCGAAGAGTTCAATCAGTACCTAACCAGCGCTCTGAACATCACCTTAGAAAGTAGCA TTTTGCTGCAAATCAAACACATAATAAGGAGCTGCCTCATGAGTGTGGAGCCCGCCATCAGCACCAAACA CCTCCCTTACCAGAGCTTCCAGCTCCTCGGCTTTGACTTCATGGTGGACGAGGAGCTGAAGGTCTGGCTC ATTGAAGTCAACGGCGCCCCAGCGTGTGCCCAGAAACTCTATGCAGAACTGTGCCAGGGCATCGTGGACA TAGCCATATCCAGTGTCTTCCCACCCCCGGACACGGAGCAGGTGCCGCAGCAGCCGGCTGCGTTCGTCAA GCTGTGAAGCACCGGAAGGCACAGAGCCACCTTGGGAAATGTACGGGGTCCTGCTCCAGAGGAAAGAGAG AGAACTGGACTGCTCCTTGTCAAGGCATGCTGGGGGACAGATGTGCAGGAAGAGTGTGAAGAGATGGCGG CTGTGGAAGAGCAGCTGAGCTGCCCACAGTGCTGTGGAGACCTTGGGAAGCTGGCGCAAGCGACTGAGAG GGAGAGCCCAGCACTTCTCTGAAGGGCTGGGACCAGGGATTTGTTATGGCGTGATCTGATCCTTTGAGCG GTTTCTGGCCTTGGATGGTACTGGGAACAGAGCGCCTTCTCCCCGGGATGCAGCCCAGGGGCCTCTGAGG GGGTGTCTGCTCACCTGCTGCAGCTTTAGTGCCTTAGTCTGTGCATGGTGCAGCCTCACTGGACACAGAC TGGACCTTAGAGCATTTTAGAGGTTGCCACATGTTCCCTGCGACACTATGTCCCGCTGAACTCTGGACCA GCATGCATCACAGTCCGGGCTGTGCCCTGGCCTGTCAGTGAAGGCCTCTTGCTTGTCACCATCCCGGTGG GCTATGAGTGAGCGCCTGCCAGGGGTCCTGTGGACGCCAGTGCATGCTGCTCACTGCATGTTTTAGAAAC AAAAGCCGCAGGCCTCACGAATCTGCAAGCATCAAATAAGCATGAGAGAGAGAAAAACAAAAGCAAAACC AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3597859 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3597859 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10982 same embryo
	EMAGE:10981 same embryo
	EMAGE:10984 same embryo
	EurExpress:euxassay_003613 same experiment
	MGI:4828997 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source