EMAGE mouse gene expression spatial database

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EMAGE:10991

Asb6 ankyrin repeat and SOCS box-containing 6 ( MGI:1919573)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003096. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003096_01	euxassay_003096_02	euxassay_003096_03	euxassay_003096_04

euxassay_003096_05	euxassay_003096_06	euxassay_003096_07	euxassay_003096_08	euxassay_003096_09

euxassay_003096_10	euxassay_003096_11	euxassay_003096_12	euxassay_003096_13	euxassay_003096_14

euxassay_003096_15	euxassay_003096_16	euxassay_003096_17	euxassay_003096_18	euxassay_003096_19

euxassay_003096_20	euxassay_003096_21	euxassay_003096_22	euxassay_003096_23	euxassay_003096_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10991_wholemount_strong.wlz

10991_wholemount_moderate.wlz

10991_wholemount_weak.wlz

10991_wholemount_possible.wlz

10991_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10991_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4714
Entity Detected:	Asb6, ankyrin repeat and SOCS box-containing 6 ( MGI:1919573)
Sequence:	sense strand is shown >T4714 CCACGCGTCCGCCCACGCGTCCGGGAGGATTGCTCACCGGCGCGGGGTCTGAACCGGTAGATCGGGAGGT GGTGGCCTGTGGGTGTCAGAACACGCGGCCGACGCCATGCCGTTCTTGCACGGCTTCCGCAGGATCATCT TTGAGTACCAGCCCCTAGTGGACGCCATCCTTGGCGCCCTGGGCATCCAGGATCTGGAGCGGCAGGAGCC CCTGGATGATTCTGCTTCCAGCGAGGAGAGCCGGATCCTGGTCCTCACGGAGCTGCTGGAGCAGAAGGCT CACTCTCCATTCTACCAGGAAGGCGTGAGCAATGCGTTGCTGAAGATGGCTGAGCTGGGCCTGACCCGGG CAGCTGCTGTCCTTCTGCAGAGTGGGGCCAACCTCAATTTCGAAGACCCTGTTACCTACTACACAGCCCT GCACATTGCTGTCCTGAGAAACCAGCCTGACATGGTTGAGCTGCTGGTGCGCCACGGGGCTGACATCAAC AGGAGGGACCGGATCCATGAGAGCAGCCCCTTGGATCTGGCCAGCGAGGAACCCGAACGCCTGCCCTGCC TGCAGCGCCTCTTGGATCTTGGAGCAGATGTCAATGCAGCTGACAAGAATGGGAAGACAGCTTTACTTCA CGCCCTGGCCAGCAGCGATGGTGTGCAGATCCACAACACAGATAACATCCGGCTCCTCCTGGAGGGAGGG GCAGACGTCAAGGCCACCACCAAGGATGGGGACACTGTATTCACCTGCATCATCTTCCTACTCGGTGAGA CTGTCTGTGGGGACAAGGAGGAGGCCCCGATGATCAACCGCTTCTGCTTCCAAGTCACGCAGCTCTTGCT GGCCCACGGTGCCGACCCCAGCGAGTGCCCGGCCCATGAGTCCCTCACGCACATCTGCCTCAAGAGCTTC AAGCTGCACTTCCCACTCCTCTGCTTCCTGCTGGAGTCCGGAGCCGCCTACAACTGCTCCCTGCACGGTG CATCCTGTTGGTCTGGCTTCAACCTCGTTTTTGAGAGGCTCTGCTCGCACCCGGGCTGTGCCGAGGACGA CAGCCACATTGAGCTTCTGCATAAGGCTGAGACCGTGCTGGACCTCATGGTGACCAGCTCCCAGAGGCTG CAGCTGCCTGAGAACCTCAACATCCACCCAGTGGGTAGCCTGGCAGGGAAGATCCAGGCCCTTCATGCCT CCCTGAGGCAGCTCGAGAGCTACCCGCCACCTCTCAAACACCTGTGCCGGGTGTCCATCCGGCTGTGCCT GCGACCGTGGCCTGTGGACACCAAGGTCAAAGCACTGCCCTTGCCCGACAGGCTCAAGTGGTACCTGCTC AGTGCACACAGTGATACCCAAGACACTTGCTGACAAGTCCCAGGGTGGAATGATCTGGGGTACCTGAAAC CAGCCTGAGGATAGACCTGGCACCTATAATGGCCTTTGGGGGAGGGGTCCCTTTTATCTGGGAAAGGCCT GCCCTTTACTGCTATGAAACCACTAGGGTGGTGCCCTGGAGGCCAAAGGCATGGAAGGCTGCTCCAGGGA GATCCCCGCCCCCTCCCTCTCAGTGTTTCACAATGGGAGGGGCCAGTGCAGATGGGACACAGCTGCCCTC TGTATTAGGAGCTGACCAGGCTGTGCACAGGGAATGAGGCCCAGGCCCCTCAGAGCCCTGGCAGCTGTGG TTCGTCCCATGGCGGAGGCACATGCCTGGGGTTTCCCAGGACCAGTGCCAAAAATCCTGGGCCATTCAGT AAGGCCCTACCCATTCATTCTGCCGCCTCGGGTCTCATGATGCCTTTGAGGAGCTGGGGACAGAACTGAG GTGGCCACAGGCTGGTCTTCTGGGTTAGAGGCCTGCCCAGTCCTCTGGTGGTCAGGGCTCTGGCACTGTG GTTGGTGGTAGCTCTGCTCACGCCACACCCAGGTCTCGGGGCTGGTGCCTTCTGTTTAAAGGCATCACGA GCACAGCAGGATTTATGAGGGGCCCAGAAGACACTGCTGAGGCCTGAAGCTGGCTCCACTCTAGTGCCAA GCCCTCACGAGATCTTCGCAGTCAGCTTGGCTTTTGGGAATGAGCAGAGATGATTAAATATATTTATAAG ATTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5035395 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5035395 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10993 same embryo
	EMAGE:10994 same embryo
	EMAGE:10995 same embryo
	EMAGE:10992 same embryo
	EurExpress:euxassay_003096 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source