EMAGE mouse gene expression spatial database

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EMAGE:11029

Nme7 non-metastatic cells 7, protein expressed in (nucleoside-diphosphate kinase) ( MGI:2449121)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003414. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003414_01	euxassay_003414_02	euxassay_003414_03	euxassay_003414_04

euxassay_003414_05	euxassay_003414_06	euxassay_003414_07	euxassay_003414_08	euxassay_003414_09

euxassay_003414_10	euxassay_003414_11	euxassay_003414_12	euxassay_003414_13	euxassay_003414_14

euxassay_003414_15	euxassay_003414_16	euxassay_003414_17	euxassay_003414_18	euxassay_003414_19

euxassay_003414_20	euxassay_003414_21	euxassay_003414_22	euxassay_003414_23	euxassay_003414_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11029_wholemount_strong.wlz

11029_wholemount_moderate.wlz

11029_wholemount_weak.wlz

11029_wholemount_possible.wlz

11029_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11029_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

oral region

weak

regional

weak expression: see section 20

submandibular gland primordium

moderate

regional

moderate expression: see section 05 06 07 08 15 16 17 18 19

pancreas

moderate

regional

moderate expression: see section 09 weak expression: see section 08 10

vibrissa

moderate

regional

moderate expression: see section 03 04 05 06 19 20 21

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12

telencephalon ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 10 11 12 13 14 15 16 17 18 19 20 21 22

4th ventricle

moderate

regional

moderate expression: see section 01 02 05 13 17 18 21 weak expression: see section 03 04 06 07 09 14 15 16 19 20

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 20 21 22 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 10 14 15 16 17 18

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 11 14 15

tongue

moderate

spotted

moderate expression: see section 10 11 12 13 14 15 16 17

lower jaw incisor

moderate

spotted

moderate expression: see section 10 11 14 15 16

lower jaw molar

moderate

spotted

moderate expression: see section 05 06 17 18 19

oral cavity

moderate

spotted

moderate expression: see section 13

oral epithelium

moderate

spotted

moderate expression: see section 04 05 06 07 08 10 11 12 14 15 16 17 18 19

upper jaw incisor

moderate

spotted

moderate expression: see section 10 11 14 15

upper jaw molar

moderate

spotted

moderate expression: see section 05 06 17 18 19

metanephros

moderate

regional

moderate expression: see section 06 07 08 09 10 16 17 18 19 20

lung

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5949
Entity Detected:	Nme7, non-metastatic cells 7, protein expressed in (nucleoside-diphosphate kinase) ( MGI:2449121)
Sequence:	sense strand is shown >T5949 TACAAACTATGCTTGCAGACCCTTATCGACGTGGTGCTGTTTCAACACTAACTTGGATCTGAATCTCCCT GAGTGCTGGAGTCAGTTCTCCCAAGCGGTGGAGTTCTACCTTGCATCTCCTACCAGGACCGCTTAGTAAT AGCTGTCTGCTGGCTCAGCGTCTAGTTCATTTCATACTTTCCAGATCACTGTGTTTTAGCAGCAGCTGGA GTTGCATCCTCGGGCAGTATGAGAGCCTGTCAGCAGGGAAGAAGTTCCAGTTTGGTTTCTCCATATATGG CACCCAAGAATCAGAGCGAGAGATTCGCTTTCATTGCAGAGTGGTATGATCCAAATGCTTCATTGCTCCG ACGCTATGAGCTGCTGTTTTACCCCACAGACGGATCTGTTGAAATGCATGATGTAAAGAATCGTCGCACC TTCTTAAAGCGGACCAAGTATGAGGACCTGCGCCTGGAAGATCTATTTATAGGCAACAAAGTCAATGTGT TTTCTCGACAGCTGGTGTTGATTGACTATGGGGACCAATACACAGCCCGCCAGCTGGGCAGCAGGAAAGA GAAAACTTTAGCCCTGATCAAACCAGATGCAGTGTCAAAGGCCGGAGAAATCATTGAGATGATAAACAAA AGTGGATTTACTATAACCAAACTCCGAATGATGACTCTGACAAGGAAAGAAGCAGCGGACTTTCATGTAG ACCATCACTCAAGGCCTTTTTATAACGAACTGATCCAGTTTATCACAAGTGGGCCTGTTATTGCCATGGA GATCTTAAGAGATGACGCGATCTGTGAGTGGAAAAGGTTGCTTGGACCCGCAAACTCTGGGCTATCACGG ACAGATGCCCCCGGAAGCATCCGAGCCCTCTTTGGGACAGATGGCGTGAGAAATGCAGCTCACAGCCCTG ATACTTTTGCATCTGCTGCCAGAGAAATGGAATTGTTTTTTCCTTCAAGTGGAGGCTGTGGGCCAGCGAA CACTGCTAAATTTACCAATTGCACCTGTTGCATCATTAAGCCTCATGCTATCAGTGAAGGAATGTTGGGA AAGATTTTAATAGCTATTCGGGATGCATGCTTTGGAATGTCAGCGATACAGATGTTCAATTTGGATCGGG TTAATGTTGAAGAATTCTATGAAGTCTATAAAGGTGTAGTGTCTGAGTATAATGATATGGTGACAGAGCT GTGCTCCGGCCCTTGCGTAGCAATAGAGATCCAACAGAGCAACCCTACAAAGACATTTCGAGAATTCTGC GGACCTGCTGATCCTGAAATCGCCCGGCATTTACGACCTGAGACCCTCAGGGCAATTTTTGGTAAAACTA AGGTTCAAAATGCTGTTCATTGCACGGATCTGCCGGAGGATGGGCTCCTGGAGGTCCAGTATTTCTTCAA GATCTTGGATAATTAGCTCTCCAGAGTAAAGACGACACAGACTGAGACGCCCAGACAATCGAGTCACACA CGTGAGGAATGTGTTCATTCTTTTATTGTCCGTTGTGTTAACCTGACTGACACAAGATCAACAAGAGCAC TGTACTCCTGGCAATTATTACATATGCTAGAACATGGATTTGCACTGTAGACAACACTTAACACCAGTCT ATGGGGTACTGCATTGCTTTTTATAAAGTTCAAAATAAAGATTTTTATTTTCAAACAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:3483725 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3483725 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11030 same embryo
	EMAGE:11032 same embryo
	EMAGE:11034 same embryo
	EMAGE:11033 same embryo
	EMAGE:11031 same embryo
	EurExpress:euxassay_003414 same experiment
	MGI:4826733 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source