EMAGE mouse gene expression spatial database

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EMAGE:11033

Ccnd3 cyclin D3 ( MGI:88315)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003402. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003402_01	euxassay_003402_02	euxassay_003402_03	euxassay_003402_04

euxassay_003402_05	euxassay_003402_06	euxassay_003402_07	euxassay_003402_08	euxassay_003402_09

euxassay_003402_10	euxassay_003402_11	euxassay_003402_12	euxassay_003402_13	euxassay_003402_14

euxassay_003402_15	euxassay_003402_16	euxassay_003402_17	euxassay_003402_18	euxassay_003402_19

euxassay_003402_20	euxassay_003402_21	euxassay_003402_22	euxassay_003402_23	euxassay_003402_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11033_wholemount_strong.wlz

11033_wholemount_moderate.wlz

11033_wholemount_weak.wlz

11033_wholemount_possible.wlz

11033_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11033_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5947
Entity Detected:	Ccnd3, cyclin D3 ( MGI:88315)
Sequence:	sense strand is shown >T5947 CGGACGCGTGGGCACGCGCGTCGCTTCTCCTAGGACTCGCTAACCCGCACTCTTGCTCTCACCCGTGAGC CATCGCAGGATGGAGCTGCTGTGTTGCGAGGGCACCCGGCACGCGCCCCGGGCCGGGCCCGACCCGCGGC TGCTTGGGGACCAGCGTGTCCTGCAGAGTTTACTCCGCCTGGAGGAGCGCTACGTGCCGCGAGCCTCCTA CTTCCAGTGCGTGCAAAAGGAGATCAAGCCGCACATGCGGAAGATGCTGGCATACTGGATGCTGGAGGTG TGTGAGGAGCAGCGCTGCGAGGAGGATGTCTTCCCTCTGGCTATGAACTACCTGGATCGCTACCTGTCCT GCGTCCCCACCCGAAAGGCGCAATTGCAGCTTCTAGGTACCGTCTGCCTGTTGCTGGCCTCCAAGCTGCG CGAAACCACGCCCCTGACTATTGAGAAGCTTTGCATCTATACGGACCAGGCTGTGGCTCCATGGCAGTTG CGGGAGTGGGAGGTGCTGGTCCTGGGGAAGCTCAAGTGGGACCTGGCTGCCGTGATTGCGCACGACTTCC TGGCCTTGATTCTGCACCGCCTGTCTCTGCCCAGTGACCGGCAGGCTTTGGTCAAAAAGCATGCCCAGAC CTTTTTGGCCCTCTGTGCTACAGATTACACCTTTGCGATGTATCCTCCATCCATGATCGCCACAGGCAGC ATTGGGGCAGCCGTGCTAGGCCTAGGCGCCTGCTCTATGTCTGCGGATGAGCTCACAGAGTTGCTGGCCG GGATCACAGGCACTGAAGTGGACTGCCTGCGAGCCTGCCAGGAACAGATCGAAGCTGCCCTCAGGGAGAG CCTCAGGGAAGCTGCTCAGACAGCCCCCAGCCCAGTGCCCAAAGCCCCCCGGGGCTCTAGCAGCCAGGGG CCCAGTCAGACCAGCACTCCCACAGATGTCACAGCCATTCACCTGTAGCTTGAGACAGGCCCTCTCAGGC CACCAAGCAGAGGAGGGGCCCCTGCCACCCCCTCCCTGCCTCTAGAACAATCCATGCTATATCTGAAGCC CGAGGGGGGCTCTCCTTCCCCCTCACAAAGCCCAAGGGGCCAGGTCCTGCCTATCCCCACAGTGTGCACT AAGGGGTTGCATGGTCATGAGGGGCGTCTTCATGGCCAGTCAGCTCCTCTTCCTTCCCACTCAACCAGCT TGGCTGTCCTGGGCCATGATGGTCAGAGAAATACAAACAGGTAAAATCCACACACCAGCATTTCTTTTGA GTCCCTCTTCTGTCCGGGGCTCCAACCTTCTCAGTTGCCAAAACGCCCCAGTACCTTCCAAAGGTGTTGG CCCTTGCAGGCTCCTTCGGGCATCCCGATAGAAGCTTATGAGGAGCTGCCCTAGATGGCCTCTGTGTAAT CCGTACTCCAGCTGCTCTTAGAGGGAACAGCCTAGGCTTTGGCCAGAGCAAGAACCCATACACTGTTGCT TTGCTTGCTGCTTAGCTTCTGTGATTGTGGGGTCTTAAGGGTGCCGTGGTCATTTTAATTTATTGCTTTG AATACAACTGTAAGAGGGTACAGTGAGGCCTGTACCCCACAAGTGGTGGTAACCCTGGCGGTTGCTCTTT CCCTCCCTTCTTCCCCTCTGCTACTGCTTTGTGGCCCAGGAGCTGCTACAGCCTGGGATGGGGGTCACGC CTTCCTCTCCTCACCCCTCCACCTCATCTTCATCAGAGCAGGGTTAGGTTGGGATGGATCGATGCCGTGG AGGTGACAGAGCTATCTGGAGAGAGGGCAAGCCCTAGGGTCACAGGTCTTTCCTCGGGCCACAAGGTTTG GGCTGGTGGCCCATTTCTATCATGCTGCCTTAATAAAGATTTCGGAATAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3483584 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3483584 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11030 same embryo
	EMAGE:11032 same embryo
	EMAGE:11034 same embryo
	EMAGE:11029 same embryo
	EMAGE:11031 same embryo
	EurExpress:euxassay_003402 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source