EMAGE mouse gene expression spatial database

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EMAGE:11035

Btf3l4 basic transcription factor 3-like 4 ( MGI:1915312)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006570. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006570_01	euxassay_006570_02	euxassay_006570_03	euxassay_006570_04

euxassay_006570_05	euxassay_006570_06	euxassay_006570_07	euxassay_006570_08	euxassay_006570_09

euxassay_006570_10	euxassay_006570_11	euxassay_006570_12	euxassay_006570_13	euxassay_006570_14

euxassay_006570_15	euxassay_006570_16	euxassay_006570_17	euxassay_006570_18	euxassay_006570_19

euxassay_006570_20	euxassay_006570_21	euxassay_006570_22	euxassay_006570_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11035_wholemount_strong.wlz

11035_wholemount_moderate.wlz

11035_wholemount_weak.wlz

11035_wholemount_possible.wlz

11035_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11035_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8072
Entity Detected:	Btf3l4, basic transcription factor 3-like 4 ( MGI:1915312)
Sequence:	sense strand is shown >T8072 AAGAGGTAATCCGGAGGACCCTCCCCGCTGCAGATTTGCAACAGCATGAATCAAGAAAAGTTAGCCAAAC TTCAAGCTCAGGTCCGGATAGGGGGCAAGGGTACAGCTCGCAGGAAGAAGAAGGTGGTACATAGGACAGC TACTGCTGATGACAAAAAGCTTCAGAGTTCACTAAAGAAACTGGCTGTGAACAATATAGCTGGTATTGAA GAGGTGAATATGATTAAAGACGATGGCACGGTTATTCATTTCAACAATCCCAAAGTCCAAGCTTCCCTCT CCGCTAACACCTTTGCAATTACTGGTCATGCAGAAGCCAAACCAATCACAGAAATGCTTCCTGGGATATT AAGTCAGCTTGGTGCTGACAGCTTAACGAGCCTTAGAAAGTTAGCTGAACAGTTCCCACGGCAAGTATTG GATAGTAAAGCGCCCAAACCAGAAGACATCGATGAAGAGGATGATGATGTTCCAGATCTTGTAGAAAATT TTGATGAAGCATCGAAAAATGAAGCTAACTAAAATCCTCTGGGTTTTGGAAGCTGGCATGGACTAGATTT AACAATCAGCTCTGTTGTTCCAAAGTTTTACAGACATGGAGAACACACCTGTTATTAGTTCAGTAATATA AATATTTTGTATATTAATGATGCTGTCTGCTCAGCAGTTCTCGGTCATTTGATTTTGCATTTTGCACTTC TTCCCAGGATCTATCTTCCCAGGATCTGTTCTTTTGGTCAAAATATGAAGTATTGGTACAGTTTGAGGGT GGTTTTTGTTTTTTTGGGGGGTGGAACTTTTGGTGTGTATGTATATGTATGTGTGTGGGTATGTGTGTAC ACAGTGGACAGCAGATTGGATAACAGTTATATCTTCCTATTCCTTCCTCCAATAGAAATAAAGCAAATCT TTACATTTGTTACTTTTTTTTTCCTAACAAATACTGCACATAATTGATGAAAAGTGAGTACCTCTGATTG CAGAGCTGAGGCGAGTCCTTCCGTTAGAAGCTTGGTGTTAATTTGTTGAATTAACTTTAATATTTTTATA CATTTCTCTGGATTGAAAATGTCCTGAACTATTTTGCCACCAGATTGCTGCGAGAGTCACAAGATGGGCC TCTTTGGTGTGGCTACCTTAGACTCACTTCCTAATCAGGCCCATACAATGTCCTTTTTTAGCTTTCTTCC TGGTGCCCTGCCTCCCCACATATTGGTCTTTAAATGAACTGGTCAGCCTTTGATATTACTCTTCTCTCCT GCCTAGCTTGAGACAGAAGTTCTCCATATAGAATTGAGAGAGTGCCTAATTCCTCTTTTTGTTCCTCACC TCGAAGAACAATGCCATTGTCAATGTTTTATTCCTTTCACATTTATAGTTGGTAGAGTTCAAACTGGAAT AGCTAGCATGTGCTTCCTAATTAACTTTATACTCGGCTTATCCTGTTTCCTAGGTGTTCTTGGTAGCAGG TGCAGGGGTGCCTCTTTTTCAGCAAAGTTGAACCTGGGAATGTCCTTTTGAATGAAAAGAACATGTAGGC CATATTATCTCCTAGCATAAATAGGCATTCTCTGAAATGGTACTGGCTGCAGGAGAATTTCTTCAGGCAG TTTCCTATTCTTGGCCTTGACCTTACCTCTGAGTAGATCTAATTGATTGTAGCTGCTCTTAACCCCCTCC TGCATGCTTAGGATAGTGCTTCGGGGGAACAGTGGTAAACACAGTATTTAATTGCTTACCTTCTTCCCCA GAACTTGGAGGTAAGTTATATTCATTCACTCAGGTTTTCTTGCTGTTCTGATAAAAGCTTGTGTTTTGAC ATATCAACATTTATTATAGCCAATATTTTAGGATAAGATCGAAAGAATCTGTTGTTTTCTGGCCCATCAC CAAACTAACTGCAGCTTAACCTTGCAGCATGCCAACTTTTGTTTCAAGCTAGATATCTTTCCTTGATACT TAAGATGCAAGACCAACAGCATATTGAGAGATTTACAGACATGAAGGCAAATCTCCCGTGTTCTTTTTTA TCCAAACATTTTCAACTTATTTTATAAATTTGTTGATTTTTTCCCCTGTTATGTTTTATATATATGGACG TAACAAAATAAATAACAGTAATAAAATAACAGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:30077136 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30077136 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11037 same embryo
	EMAGE:11036 same embryo
	EurExpress:euxassay_006570 same experiment
	MGI:4823516 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source