EMAGE mouse gene expression spatial database

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EMAGE:11037

Nrbp2 nuclear receptor binding protein 2 ( MGI:2385017)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006542. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006542_01	euxassay_006542_02	euxassay_006542_03	euxassay_006542_04

euxassay_006542_05	euxassay_006542_06	euxassay_006542_07	euxassay_006542_08	euxassay_006542_09

euxassay_006542_10	euxassay_006542_11	euxassay_006542_12	euxassay_006542_13	euxassay_006542_14

euxassay_006542_15	euxassay_006542_16	euxassay_006542_17	euxassay_006542_18	euxassay_006542_19

euxassay_006542_20	euxassay_006542_21	euxassay_006542_22	euxassay_006542_23	euxassay_006542_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11037_wholemount_strong.wlz

11037_wholemount_moderate.wlz

11037_wholemount_weak.wlz

11037_wholemount_possible.wlz

11037_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11037_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6810
Entity Detected:	Nrbp2, nuclear receptor binding protein 2 ( MGI:2385017)
Sequence:	sense strand is shown >T6810 GGGGAACCTGCGGGAGCCCTGGACAGAGACTGCCCAGTCTCCCCGCCCAACTGACATCCTGTCTTTCTCT CTGCGTTGGCTGTTGCAGTGTGGTACCGCATCTTCTCCAATGCACTTCCTGATGATCTCCGGAGCCCTAT TCGAGCTGAACGGGAAGAACTCCGAAACCTGCACTTTTTCCCACCAGAGTATGGCGAAGTCAATGATGGG ACTGCTGTGGACATCTTCTCCTTCGGGATGTGTGCACTGGAGATGGCTGTACTCGAGATCCAAGCCAACG GGGATACCAGAGTCACAGAAGAGGCCATCGCTCGAGCCAGGCACTCACTGAGTGACCCCAACATGCGGGA ATTCATCCTCTCCTGCCTGGCCCGGGACCCTGCCCGCCGACCCTCAGCCCACAACCTCCTCTTCCACCGA GTGCTCTTTGAGGTGCACTCGCTGAAGCTGCTGGCAGCTCACTGCTTCATCCAGCACCAGTACCTCATGC CTGAGAATGTGGTAGAGGAAAAGACCAAGGCCATGGACCTCCATGCAGTTTTGGCTGAGATGCCGCAGCC CCATGGACCCCCAATGCAGTGGCGGTACTCAGAGGTCTCCTTCTTGGAGCTGGACAAATTCCTAGAGGAT GTCAGGAACGGGATCTATCCACTGATGAACTTTGCGGCTGCTCGGCCCTTGGGGCTTCCCCGTGTGTTGG CCCCACCCCCAGAGGAAGCCCAAAAGGCCAAAACTCCAACGCCAGAACCCTTTGACTCGGAGACCAGGAA GGTGGTCCAGATGCAGTGCAACCTGGAAAGAAGCGAGGACAAGGCTCGGTGGCACCTTACTCTGCTCTTG GTGCTTGAGGACCGGCTACATCGGCAGCTGACCTATGATCTGCTCCCAACGGACAGTGCCCAGGACCTCG CTGCTGAACTAGTGCATTATGGCTTCCTGCACGAGGATGACAGGACAAAGCTAGCAGCCTTTCTGGAGAC CACTTTTCTCAAGTACCGAGGGACGCAAGCGTGACCTTCCCAGTCCTGACGGCCCAGCAGAGATACAGGG GCTCAGGGTTGTCCACTTGGCAAAGAGCCCCCACACTGCTCAAAGCTGCCTTCTGCCTGTGTTCCCTGGA ACTGAACACAGGCCCTGCTAGTGAAGACACCCCCACCCCCCAGCTTTCTGCAGCAGTGTGGGACCCTGGG GTGGTGATGGAGCCCTGAGCCTGGACGAGAGTGGATACAGGTCAGTTAGGGGAACCGCTCCATCTGGTAC TAGACAACAGCCATGCCTTCAGGTGGCATAGAAACCTAGGGAAGGAGCCTGAACTCAGGTGTCACAGTGC TGGGCATCAGGCAGACCAGACCTGACCTGATTGGAGAACTGTAGACTAGATAGCTTGGAGTTGAACCCAT GGCCAGGGAATTCCTTGGTCCTGCTCAGACCAGTCCTGATCCCTTGCAGACCTGCCTTGAGCCCTCTTTC TGATCTTCCACACTCTTGAGACCAGGACCTGTGTCCTCCCCAAAGCCCTTGGGAAGGATCTTTCTATTCA TCATCCCTCTGGCCTAGGGGCTCAGGGGTCAGGCATCCTCCACATTCCCTCCCTGGGGAAGTTGTGTGTT TGAGTTGAGGATGTGGGTTCCTGGCTCCCTCTTTCTCCCCAGCCCAACTTGTCTCTTTCTTACTGGTTTC AAAGTCCTGATGAACGCTTCCCCTCAGAGCCACCCTGGTTTCCTTGGTTCTTGAACTGCCTCTCTCCCAA CTTCAAACCAGGTCTTAAACGTTTTTTAAATGCATATATAAATGTAATGCAGTCACGGTCCTTTTTAAAC ACTTTGTGTATGAAACCAGGAAAGCTCACTATTGTATTAGGAATAGTTCCACATTGCTGCTGTTAACAGA TATCATAAACCCAGTGGTTTGAGACGACACACACACACACACACACACACACACACAGAGAGAGAGAGAG TTCTGTACATCAAGTGTGATCCAGGCTCTCACTAGATTAATACCCAGGCTAAGTTCCTTTCTGGAAGCTG GGACTTACCTCCTGCTCCTTCAAGCTATTGGCAGAACTCACTTCCCTGCAATGGTAAGGCAGAAATCCCT ATTTTCTCAACAGCTGCCAACTAAGAACCCCTCTCAGCTTCTAGAGGCCACCAACTTTTCTTAGTTCTTC TTTCTCCCCCCTCAAGACCAGCAGCGTCAAGTTGAATCTTTGTCCTGGGCTAGCTGACTGGCTTGCCACT GCTGGGAAGAGTTGGGGCCTTTTGTGAGTAGGTTGGACCCACCAGGATAACCGAGGATGATCCCCTTCTC AGGGTCTATAGATGAACCACACCTGCGCAGTTCCTTCTGCTGTCATCCTGGGCTTTGGTGCTTGGAGAAC AGCCGTGGGCGGTGGGTGTTGTTACTGTGGTACCTACCATGCCATCTTAACCGAAACCAAGACCTAAAAT AAAACAGATTTGTCATGGGACATCTAATAAATTAAATGAACTCTGAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3710089 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3710089 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11036 same embryo
	EMAGE:11035 same embryo
	EurExpress:euxassay_006542 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source