EMAGE mouse gene expression spatial database

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EMAGE:11049

Cyp7b1 cytochrome P450, family 7, subfamily b, polypeptide 1 ( MGI:104978)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001156. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001156_01	euxassay_001156_02	euxassay_001156_03	euxassay_001156_04

euxassay_001156_05	euxassay_001156_06	euxassay_001156_07	euxassay_001156_08	euxassay_001156_09

euxassay_001156_10	euxassay_001156_11	euxassay_001156_12	euxassay_001156_13	euxassay_001156_14

euxassay_001156_15	euxassay_001156_16	euxassay_001156_17	euxassay_001156_18	euxassay_001156_19

euxassay_001156_20	euxassay_001156_21	euxassay_001156_22	euxassay_001156_23	euxassay_001156_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11049_wholemount_strong.wlz

11049_wholemount_moderate.wlz

11049_wholemount_weak.wlz

11049_wholemount_possible.wlz

11049_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11049_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme

weak

regional

weak expression: see section 06 07 08 10 11 12 17 18

kidney calyx

moderate

regional

moderate expression: see section 07 08 09 10 11 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4884
Entity Detected:	Cyp7b1, cytochrome P450, family 7, subfamily b, polypeptide 1 ( MGI:104978)
Sequence:	sense strand is shown >T4884 CCACGCGTCCGAGCGGGCAGGCCACAGCCTCTGGTCTAGAAGAGAGGGCACTGTGCAGAAGCCATCGCTC CCTACAGAGCCGCCAGCTCGTCGGGATGCAGGGAGCCACGACCCTAGATGCCGCCTCGCCAGGGCCTCTC GCCCTCCTAGGCCTTCTCTTTGCCGCCACCTTACTGCTCTCGGCCCTGTTCCTCCTCACCCGGCGCACCA GGCGCCCTCGTGAACCACCCTTGATAAAAGGTTGGCTTCCTTATCTTGGCATGGCCCTGAAATTCTTTAA GGATCCGTTAACTTTCTTGAAAACTCTTCAAAGGCAACATGGTGACACTTTCACTGTCTTCCTTGTGGGG AAGTATATAACATTTGTTCTGAACCCTTTCCAGTACCAGTATGTAACGAAAAACCCAAAACAATTAAGCT TTCAGAAGTTCAGCAGCCGATTATCAGCGAAAGCCTTCTCTGTAAAGAAGCTGCTTACTGATGACGACCT TAATGAAGACGTTCACAGAGCCTATCTACTTCTACAAGGCAAACCTTTGGATGCTCTTCTGGAAACTATG ATCCAAGAAGTAAAAGAATTATTTGAGTCCCAACTGCTAAAAATCACAGATTGGAACACAGAAAGAATAT TTGCATTCTGTGGCTCACTGGTATTTGAGATCACATTTGCGACTCTATATGGAAAAATTCTTGCTGGTAA CAAGAAACAAATTATCAGTGAGCTAAGGGATGATTTTTTTAAATTTGATGACATGTTCCCATACTTAGTA TCTGACATACCTATTCAGCTTCTAAGAAATGAAGAATCTATGCAGAAGAAAATTATAAAATGCCTCACAT CAGAAAAAGTAGCTCAGATGCAAGGACAGTCAAAAATTGTTCAGGAAAGGCAAGATCTGCTGAAAAGATA CTATAGGCATGACGATCCTGAAATAGGAGCACATCATCTTGGCTTTCTCTGGGCCTCTCTAGCAAACACC ATTCCAGCTATGTTCTGGGCAATGTATTATATTCTTCGGCATCCTGAAGCTATGGAAGCCCTGCGTGACG AAATTGACAGTTTCCTGCAGTCAACAGGTCAAAAGAAAGGACCTGGAATTTCAGTCCACTTCACCAGAGA ACAATTGGACAGCTTGGTCTGCCTGGAAAGCACTATTCTTGAGGTTCTGAGGCTGTGCTCATACTCCAGC ATCATCCGAGAAGTGCAGGAGGATATGAATCTCAGCTTAGAGAGTAAGAGTTTCTCTCTGCGGAAAGGAG ATTTTGTAGCCCTCTTTCCTCCACTCATACACAATGACCCGGAAATCTTCGATGCTCCAAAGGAATTTAG GTTCGATCGCTTCATAGAAGATGGTAAGAAGAAAAGCACGTTTTTCAAAGGAGGGAAGAGGCTGAAGACT TACGTTATGCCTTTTGGACTCGGAACAAGCAAATGTCCAGGGAGATATTTTGCAGTGAACGAAATGAAGC TACTGCTGATTATGCTTTTAACTTATTTTGATTTAGAAATTATCGACAGGAAGCCTATAGGGCTAAATCA CAGTCGGATGTTTTTAGGTATTCAGCACCCCGATTCTGCCGTCTCCTTTAGGTACAAAGCAAAATCTTGG AGAAGCTGAAAGTGTGGCAGAGAAGCTTTGCAGAGTAAGGCTGCATGTGCTGAGCTCCGTGATTTGGTGC ACTCCCCCAAATGCAACCGCTACTCTTGTTTGAAAATGGCAAATTTATATTTGGTTGAGATCAATCCAGT TGGTTTTGGGTCACAAAACCTGTCATAAAATAAAGCAGTGTGATGGTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5098739 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5098739 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11053 same embryo
	EMAGE:11050 same embryo
	EMAGE:11052 same embryo
	EMAGE:11051 same embryo
	EurExpress:euxassay_001156 same experiment
	MGI:4824182 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source