EMAGE mouse gene expression spatial database

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EMAGE:11079

Ace angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 ( MGI:87874)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003654. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003654_01	euxassay_003654_02	euxassay_003654_03	euxassay_003654_04

euxassay_003654_05	euxassay_003654_06	euxassay_003654_07	euxassay_003654_08	euxassay_003654_09

euxassay_003654_10	euxassay_003654_11	euxassay_003654_12	euxassay_003654_13	euxassay_003654_14

euxassay_003654_15	euxassay_003654_16	euxassay_003654_17	euxassay_003654_18	euxassay_003654_19

euxassay_003654_20	euxassay_003654_21	euxassay_003654_22	euxassay_003654_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11079_wholemount_strong.wlz

11079_wholemount_moderate.wlz

11079_wholemount_weak.wlz

11079_wholemount_possible.wlz

11079_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11079_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 04 05 06 07 08 09 20 21 22 23

3rd ventricle

strong

regional

strong expression: see section 12 13

choroid invagination

strong

regional

strong expression: see section 05 06 07 08 09 10 11 15 16 17 18 19

4th ventricle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

aorta

strong

regional

strong expression: see section 07 08 09 10 11 12 13

mesenteric artery

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

umbilical artery embryonic part

strong

regional

strong expression: see section 13 14

heart

strong

regional

strong expression: see section 11 12 13

metanephros

strong

spotted

strong expression: see section 06 07 08 09 17 18 19

lung

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6865
Entity Detected:	Ace, angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 ( MGI:87874)
Sequence:	sense strand is shown >T6865 CCCACGCGTCCGGCGCGCACCGCGCCATGGGGGCCGCGTCCGGCCAGCGGGGGCGGTGGCCGTTGTCGCC TCCGCTCTTGATGCTGTCGCTGCTGGTGCTGTTGCTGCAGCCGTCGCCCGCCCCGGCACTCGACCCTGGA TTGCAGCCGGGCAACTTTTCCCCGGACGAGGCAGGGGCGCAGCTTTTCGCTGAAAGCTATAACTCGAGTG CCGAGGTGGTGATGTTCCAGAGCACCGTGGCCAGTTGGGCGCACGACACCAACATCACGGAGGAGAACGC GCGACGCCAGGAGGAAGCGGCCCTGGTCAGCCAGGAGTTTGCAGAGGTCTGGGGCAAGAAGGCCAAGGAG TTGTATGAGTCCATTTGGCAGAACTTTACTGACTCAAAGCTGCGAAGGATCATCGGATCTATTCGGACCC TAGGACCTGCCAATCTGCCCCTGGCCCAGCGGCAGCAGTACAACTCTCTGCTAAGCAACATGAGCAGAAT CTACTCCACTGGCAAGGTCTGCTTCCCCAACAAGACTGCCACCTGCTGGTCCCTGGACCCAGAGCTCACC AACATCCTGGCTTCCTCACGAAGCTATGCCAAGTTGTTGTTTGCCTGGGAGGGCTGGCATGATGCTGTGG GTATCCCACTGAAACCCCTCTATCAAGACTTTACTGCCATCAGTAACGAAGCCTACAGACAAGACGACTT CTCAGACACAGGAGCCTTCTGGCGCTCCTGGTATGAGTCCCCCTCCTTTGAGGAGAGTCTGGAACATATC TACCACCAACTAGAGCCCCTCTACCTGAACCTCCATGCCTACGTCCGCCGCGCACTGCACCGCCGCTATG GGGACAAATACGTCAATCTCAGGGGGCCTATTCCTGCCCATTTGCTGGGAGACATGTGGGCCCAGAGCTG GGAGAACATCTACGACATGGTAGTGCCTTTCCCAGACAAACCCAACCTCGATGTCACCAGTACAATGGTA CAGAAGGGCTGGAACGCCACACACATGTTCCGGGTATCAGAGGAATTCTTCACCTCGCTGGGGCTCTCAC CCATGCCTCCTGAGTTCTGGGCGGAGTCAATGCTGGAGAAACCAACGGATGGACGGGAGGTGGTGTGCCA CGCCTCTGCCTGGGACTTCTACAACCGGAAGGACTTCCGGATTAAGCAATGCACACGGGTCACGATGGAA CAGCTGGCCACAGTACACCACGAGATGGGCCACGTGCAGTACTACCTCCAGTACAAGGACCTGCACGTCT CTCTGCGTAGAGGTGCCAACCCTGGCTTCCATGAGGCCATTGGGGATGTGCTTGCACTCTCCGTCTCTAC CCCTGCACATCTGCACAAAATCGGCCTACTGGACCATGTTACCAATGACATAGAGAGTGACATCAATTAC CTGCTAAAGATGGCCCTAGAGAAAATCGCCTTCTTGCCCTTTGGCTACCTGGTGGACCAGTGGCGTTGGG GGGTCTTCAGTGGACGGACCCCACCCTCTCGCTACAACTTCGACTGGTGGTATCTTCGAACCAAGTATCA GGGGATCTGCCCACCAGTTGCCCGGAATGAAACCCATTTTGATGCTGGAGCCAAGTTTCACATCCCAAAC GTGACACCGTACATCAGGTACTTCGTGAGCTTTGTGCTGCAGTTCCAGTTCCATCAAGCACTGTGCAAGG AGGCAGGCCACCAGGGCCCACTACACCAGTGTGACATCTACCAGTCCGCCCAGGCGGGGGCCAAGCTCAA GCAGGTGTTGCAGGCTGGCTGCTCCAGGCCCTGGCAGGAGGTACTGAAGGACCTGGTAGGCTCAGATGCC CTGGATGCCAAGGCACTGCTGGAGTACTTCCAACCGGTCAGCCAGTGGCTGGAAGAGCAGAATCAGCGGA ATGGCGAAGTCCTAGGCTGGCCAGAGAATCAGTGGCGTCCACCGTTACCCGACAACTATCCAGAGGGCAT TGACCTAGAGACTGATGAAGCCAAGGCTGACAGGTTCGTGGAAGAGTATGACCGGACAGCCCAAGTGTTG TTGAACGAGTACGCAGAGGCCAACTGGCAATATAACACCAACATTACCATAGAGGGCAGCAAGATCCTGC TTGAGAAAAGCACGGAGGTATCCAATCACACCCTGAAATATGGCACCCGGGCCAAGACATTTGATGTGAG CAACTTTCAAAACTCTTCCATCAAGCGGATCATAAAGAAGCTTCAGAACCTGGACCGGGCAGTGCTGCCT CCCAAGGAATTAGAAGAGTACAACCAGATCCTGCTAGACATGGAGACAACTTACAGCTTATCCAACATTT GCTACACAAATGGCACTTGTATGCCCCTGGAACCTGATCTAACAAACATGATGGCCACATCCCGGAAATA TGAAGAATTGCTATGGGCATGGAAGAGCTGGAGAGACAAGGTGGGGAGAGCCATCCTTCCTTTTTTCCCA AAGTATGTGGAGTTCTCCAACAAGATTGCCAAGCTCAATGGCTACACGGATGCAGGGGATTCATGGAGAT CCTTATACGAGTCTGACAACCTGGAGCAAGACCTGGAAAAACTGTACCAGGAGCTGCAGCCACTCTACCT GAACCTGCATGCCTATGTGCGTCGTTCCCTGCACCGCCACTATGGGTCCGAGTACATCAACCTGGATGGC CCCATTCCTGCCCATCTGCTAGGGAACATGTGGGCGCAGACCTGGTCCAACATCTATGATTTGGTGGCGC CCTTCCCTTCCGCCCCCAATATAGATGCCACGGAAGCCATGATAAAGCAGGGATGGACACCCAGAAGGAT ATTTAAGGAAGCTGACAATTTCTTTACCTCCCTGGGGCTGTTACCTGTGCCCCCTGAGTTCTGGAACAAG TCGATGTTAGAGAAGCCCACCGATGGAAGGGAGGTGGTGTGCCATCCCTCAGCCTGGGACTTCTACAACG GCAAGGACTTCAGGATCAAGCAGTGTACCTCTGTGAACATGGAGGACTTGGTGATAGCGCACCACGAAAT GGGCCACATCCAGTATTTCATGCAGTACAAAGACTTACCCGTGACTTTCCGGGAGGGTGCCAACCCTGGT TTTCATGAAGCTATTGGAGATATAATGGCTCTCTCAGTGTCTACCCCCAAGCATCTATACAGTCTCAACC TGCTTAGCACTGAGGGCAGTGGCTACGAGTATGACATCAACTTTCTAATGAAGATGGCCCTCGACAAGAT CGCCTTTATCCCCTTCAGCTACCTCATCGACCAGTGGCGCTGGAGGGTCTTTGATGGAAGCATCACCAAG GAGAACTATAACCAGGAGTGGTGGAGCCTCAGGCTGAAGTATCAGGGTCTGTGCCCCCCAGTGCCAAGAT CCCAAGGTGACTTTGACCCAGGGTCCAAGTTCCACGTTCCTGCGAACGTGCCATACGTCAGGTACTTTGT CAGCTTCATCATCCAGTTCCAGTTCCACGAGGCGCTGTGTCGCGCAGCCGGGCACACGGGTCCCCTGCAC AAGTGTGACATCTACCAATCCAAGGAAGCAGGGAAGCTCCTGGCGGATGCCATGAAGCTGGGCTACAGTA AGCCGTGGCCAGAGGCCATGAAGCTGATCACAGGCCAGCCTAACATGTCAGCCTCCGCCATGATGAATTA CTTCAAGCCACTGACAGAATGGCTCGTCACCGAGAACAGGAGACATGGAGAGACACTGGGCTGGCCGGAG TACAACTGGGCGCCAAACACCGCTCGCGCAGAAGGCTCCACCGCAGAGTCCAACCGCGTCAATTTCCTGG GCCTGTACCTGGAGCCACAGCAGGCCCGCGTGGGCCAGTGGGTGCTGCTCTTCCTGGGCGTCGCCCTGCT CGTGGCCACCGTGGGTCTCGCCCATCGGCTCTACAACATCCGTAACCATCACAGCCTCCGCCGGCCCCAC CGTGGGCCCCAGTTTGGGTCTGAGGTGGAGCTCAGACACTCCTGAGGTGACCCTGCTGGCAAGGCCAGCA GAGGAGTGTCCCATAAGAAACTGGATGGGGGACATGGTGTTTGAGTGGAACATACCCTAGCTGAGCCTTC CTCCTTTGCTGTCCCCATCCACTCTGCCCCACCCCCACCCCCCAGGCCCAGCCCCCATTCTCTGGATACC CAGTGTCTAACACCGACTTCCCTGCCCAGTCTCTGTGAATACAATTAAAGGTCCTCCCCCAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:3965649 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3965649 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11081 same embryo
	EMAGE:11078 same embryo
	EMAGE:11082 same embryo
	EMAGE:11080 same embryo
	EMAGE:11077 same embryo
	EurExpress:euxassay_003654 same experiment
	MGI:4822915 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source