EMAGE mouse gene expression spatial database

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EMAGE:11080

Nupl1 nucleoporin like 1 ( MGI:1919094)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003663. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003663_01	euxassay_003663_02	euxassay_003663_03	euxassay_003663_04

euxassay_003663_05	euxassay_003663_06	euxassay_003663_07	euxassay_003663_08	euxassay_003663_09

euxassay_003663_10	euxassay_003663_11	euxassay_003663_12	euxassay_003663_13	euxassay_003663_14

euxassay_003663_15	euxassay_003663_16	euxassay_003663_17	euxassay_003663_18	euxassay_003663_19

euxassay_003663_20	euxassay_003663_21	euxassay_003663_22	euxassay_003663_23	euxassay_003663_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11080_wholemount_strong.wlz

11080_wholemount_moderate.wlz

11080_wholemount_weak.wlz

11080_wholemount_possible.wlz

11080_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11080_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

testis

strong

regional

strong expression: see section 04 05 06 07 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6854
Entity Detected:	Nupl1, nucleoporin like 1 ( MGI:1919094)
Sequence:	sense strand is shown >T6854 GCCGCACCGCCGCTTGGGGTTGTTTCCCACCCGGTACCCGCTGGGCACCAGCTATGTGGCACGTCCCTCT TCGGCAGTGAAGGGACAGAAGCGTCCTAGGCCTCTCCACCCTAGCTGACGCCTTCGCGCCAAGGGCCGCC ATGGCCACGGGGTTCTCCTTCGGGTCTGGGACCCTCGGCTCCACCACTGTGGCCCCCGGCGGGACCGGAG CGGGCAGCGGCTTCTCCTTCGGGACCGTCGCGTCTAGCACACCTTCTGTGGGACTCAACTTTGGATCTCT TGGAAGCTCTGTGACCCCAGCATCTACATCTGCTTCAGCTGGTGGTTTTGGAACAGGCCTCTTTGGATCT AAGCCTGCCACTGGATTTACTCTAGGAGGAACAAGTGCAGGAACAGCAGCAACAACATCTGCGTCTACAA CAGGCTTCAGTTTAGGATTCAGTAAACCTGCGGCATCTGCCACACCATTTGCCCTGCCTGTCACTTCTAC CTCAGCTAGTGGTCTTACTCTCTCATCTGCTCTGACATCAACTCCAGCAGCATCCACAGGATTTACTTTG AATAACTTGGGAGCAACACCAGCCACAACTACAACAGCATCAACTGGGCTTTCTTTAGGGGGAGCCTTAG CTGGTTTGGGAGGTTCACTTTTTCAGAGTGGAAACACAGCAACATCAGGTCTCGGACAAAATGCTTTAAG CTTGTCTTTGGGAACTACAGCACCTACTTCAGCTGCCAGCAATGAAGGCCTTGGTGGTATAGACTTTAGT ACCTCATCAGATAAGAAGAGTGATAAAACGGGAACAAGGCCAGAGGACAGTAAAGCACTGAAAGATGAAA ACCTGCCTCCTGTCATCTGTCAGGATGTTGAAAACCTCCAGAAATTTGTCAAGGAACAGAAACAAGTCCA AGAAGAAATTAGCCGAATGTCTTCCAAAGCAATGCTTAAAGTCCAGGAAGACATCAAGGCCCTGAAGCAG CTTCTGTCATTGGCTGCCAGTGGGTTGCAGAGGAATACACTCAATATTGACAAACTGAAGTTAGAAACTG CTCAGGAGTTAAAGAATGCCGAAATAGCTTTAAGAACCCAGAAAACACCACCTGGACTCCAACATGAAAA CACAGCTCCTGCTGACTACTTCAGAATATTGGTTCAGCAGTTTGAGGTACAGCTTCAGCAGTACAGGCAG CAGATTGAAGAACTAGAAAACCATCTTGCCACTCAAGCCAGTAATTCACATATAACTCCTCAAGATTTAT CAATGGCTATGCAGAAAATTTATCAAACATTTGTAGCTTTAGCCGCACAACTTCAATCTATTCATGAAAA TGTCAAGGTGCTTAAAGAACAGTATCTCGGCTACAGAAAAATGTTTTTGGGTGATGCTGTGGATGTGTTT GAAGCCAGGAGAACAGAAGCCAAGAAGTGGCAGAATGCACCCAGAGTTACTACTGGACCCACTCCTTTCA GCACCATGCCAAACGCAGCAGCCGTTGCCATGGCTGCAACACTTACACAGCAGCAACAGCCTGCTACAGG GCCACAGCCGTCTCTGGGAGTTAGTTTTGGAACGCCATTCGGCTCAGGTATTGGCACTGGCTTGCAATCA AGTGGCTTAGGTTCTTCAAACCTTGGAGGATTTGGAACTAGCTCTGGTTTCGGATGCGGTACCACAGGGG CCTCCACATTTGGATTTGGAACAACAGATAAACCCTCAGGAAGTCTTAGTGCAGGCTTTGGCAGCTCAAG TACGTCTGGGTTTAACTTCAGCAATCCTGGCATCACGGCATCAGCTGGTTTGACATTTGGGGTATCCAAT CCTGCATCTGCGGGGTTTGGAACAGGAGGACAACTCCTTCAATTGAAGAGACCTCCAGCTGGAAACAAAA GAGGAAAGAGATAAACATACGGGTCGATGTGTTGGCCGTGCTTAGCTGGCTGCCGCTCATTCTATGATTC TATTTTTCTAACGATGTAGTAATTGAACTGCATCTCGGGAGATTTATAACATTTTGATATTCCTCACTCT GGAACTTGAACTTTGTTTACCTTACCAAACATCTTTCTTGTGAATTCAAAATCCAGTTGCAGTTTTGGTT GCTCTTTTTTTTGATTCTCCATGTAAGAAATGTTGTGATTAAATTACCAATTGATAGTGGTGGGAAAAAA ATCCATCAACCTAAGCCTATGATCTATCCTAGTACTAATAGCAATGTTTCTGTTGCTGAGGTTTATGCTG TGAATACATGCACATTGGTTTCTTATGCAGATGGTATAAACTCTAGGGCCTGTGCTAGAAGTAGTTTGTT CCTTGACAAGGCCTTTTGAACTGTGCTGCAGGGCATCTTGTGGATATGTATGTAATTATATAGAGAGTAA TGCACACAATGTGTGCACATAAAATATATATTTACACACCTACAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3964978 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3964978 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11081 same embryo
	EMAGE:11079 same embryo
	EMAGE:11078 same embryo
	EMAGE:11082 same embryo
	EMAGE:11077 same embryo
	EurExpress:euxassay_003663 same experiment
	MGI:4826835 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source