EMAGE mouse gene expression spatial database

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EMAGE:11082

Irf1 interferon regulatory factor 1 ( MGI:96590)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003661. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003661_01	euxassay_003661_02	euxassay_003661_03	euxassay_003661_04

euxassay_003661_05	euxassay_003661_06	euxassay_003661_07	euxassay_003661_08	euxassay_003661_09

euxassay_003661_10	euxassay_003661_11	euxassay_003661_12	euxassay_003661_13	euxassay_003661_14

euxassay_003661_15	euxassay_003661_16	euxassay_003661_17	euxassay_003661_18	euxassay_003661_19

euxassay_003661_20	euxassay_003661_21	euxassay_003661_22	euxassay_003661_23	euxassay_003661_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11082_wholemount_strong.wlz

11082_wholemount_moderate.wlz

11082_wholemount_weak.wlz

11082_wholemount_possible.wlz

11082_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11082_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 10 11 12 13 14 15

midgut

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

testis

strong

regional

strong expression: see section 04 05 06 07 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6657
Entity Detected:	Irf1, interferon regulatory factor 1 ( MGI:96590)
Sequence:	sense strand is shown >T6657 CCCAGAGTGGCCGTCGCGCGCACCCGCGAGCAGCGCCGGGGGACTCGGATGTCCGCCTGCGCGCACTTTT GGCGGTCGCAGCTTGGCTCCCAGCTCTTGCTTTCGGACGCGCAGTGTTCGGTGCAGAGGTTGGCTCGCTG CCTTGACTGGCTGGACCGGGTCGTGAACTACTGGGCTTTCGGGAGGAGGTGCCACAGCCACCATGCCAAT CACTCGAATGCGGATGAGACCCTGGCTAGAGATGCAGATTAATTCCAACCAAATCCCAGGGCTGATCTGG ATCAATAAAGAAGAGATGATCTTCCAGATTCCATGGAAGCACGCTGCTAAGCACGGCTGGGACATCAACA AGGATGCCTGTCTGTTCCGGAGCTGGGCCATTCACACAGGCCGATACAAAGCAGGAGAAAAAGAGCCAGA TCCCAAGACATGGAAGGCAAACTTCCGTTGTGCCATGAACTCCCTGCCAGACATCGAGGAAGTGAAGGAT CAGAGTAGGAACAAGGGCAGCTCTGCTGTGCGGGTGTACCGGATGCTGCCACCCCTCACCAGGAACCAGA GGAAAGAGAGAAAGTCCAAGTCCAGCCGAGACACTAAGAGCAAAACCAAGAGGAAGCTGTGTGGAGATGT TAGCCCGGACACTTTCTCTGATGGACTCAGCAGCTCTACCCTACCTGATGACCACAGCAGTTACACCACT CAGGGCTACCTGGGTCAGGACTTGGATATGGAAAGGGACATAACTCCAGCACTGTCACCGTGTGTCGTCA GCAGCAGTCTCTCTGAGTGGCATATGCAGATGGACATTATACCAGATAGCACCACTGATCTGTATAACCT ACAGGTGTCACCCATGCCTTCCACCTCCGAAGCCGCAACAGACGAGGATGAGGAAGGGAAGATAGCCGAA GACCTTATGAAGCTCTTTGAACAGTCTGAGTGGCAGCCGACACACATCGATGGCAAGGGATACTTGCTCA ATGAGCCAGGGACCCAGCTCTCTTCTGTCTATGGAGACTTCAGCTGCAAAGAGGAACCAGAGATTGACAG CCCTCGAGGGGACATTGGGATAGGCATACAACATGTCTTCACGGAGATGAAGAATATGGACTCCATCATG TGGATGGACAGCCTGCTGGGCAACTCTGTGAGGCTGCCGCCCTCTATTCAGGCCATTCCTTGTGCACCAT AGTTTGGGTCTCTGACCCGTTCTTGCCCTCCTGAGTGAGTTAGGCCTTGGCATCATGGTGGCTGTGATAC AAAAAAAGCTAGACTCCTGTGGGCCCCTTGACACATGGCAAAGCATAGTCCCACTGCAAACAGGGGACCA TCCTCCTTGGGTCAGTGGGCTCTCAGGGCTTAGGAGGCAGAGTCTGAGTTTTCTTGTGAGGTGAAGCTGG CCCTGACTCCTAGGAAGATGGATTGGGGGGTCTGAGGTGTAAGGCAGAGGCCATGGACAGGAGTCATCTT CTAGCTTTTTAAAAGCCTTGTTGCATAGAGAGGGTCTTATCGCTGGGCTGGCCCTGAGGGGAATAGACCA GCGCCCACAGAAGAGCATAGCACTGGCCCTAGAGCTGGCTCTGTACTAGGAGACAATTGCACTAAATGAG TCCTATTCCCAAAGAACTGCTGCCCTTCCCAACCGAGCCCTGGGATGGTTCCCAAGCCAGTGAAATGTGA AGGGAAAAAAAATGGGGTCCTGTGAAGGTTGGCTCCCTTAGCCTCAGAGGGAATCTGCCTCACTACCTGC TCCAGCTGTGGGGCTCAGGAAAAAAAATGGCACTTTCTCTGTGGACTTTGCCACATTTCTGATCAGAGGT GTACACTAACATTTCTCCCCAGTCTAGGCCTTTGCATTTATTTATATAGTGCCTTGCCTGGTGCCTGCTG TCTCCTCAGGCCTTGGCAGTCCTCAGCAGGCCCAGGGAAAAGGGGGGTTGTGAGCGCCTTGGCGTGACTC TTGACTATCTATTAGAAACGCCACCTAACTGCTAAATGGTGTTTGGTCATGTGGTGGACCTGTGTAAATA TGTATATTTGTCTTTTTATAAAAATTTAAGTTGTTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3600525 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3600525 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11081 same embryo
	EMAGE:11079 same embryo
	EMAGE:11078 same embryo
	EMAGE:11080 same embryo
	EMAGE:11077 same embryo
	EurExpress:euxassay_003661 same experiment
	MGI:4825618 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source