EMAGE mouse gene expression spatial database

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EMAGE:11105

Slc7a9 solute carrier family 7 (cationic amino acid transporter, y+ system), member 9 ( MGI:1353656)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019720. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019720_01	euxassay_019720_02	euxassay_019720_03	euxassay_019720_04

euxassay_019720_05	euxassay_019720_06	euxassay_019720_07	euxassay_019720_08	euxassay_019720_09

euxassay_019720_10	euxassay_019720_11	euxassay_019720_12	euxassay_019720_13	euxassay_019720_14

euxassay_019720_15	euxassay_019720_16	euxassay_019720_17	euxassay_019720_18	euxassay_019720_19

euxassay_019720_20	euxassay_019720_21	euxassay_019720_22	euxassay_019720_23	euxassay_019720_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11105_wholemount_strong.wlz

11105_wholemount_moderate.wlz

11105_wholemount_weak.wlz

11105_wholemount_possible.wlz

11105_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11105_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

midgut

weak

regional

weak expression: see section 16 17 18 19

metanephros

moderate

regional

moderate expression: see section 09 21 weak expression: see section 10 11 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T56043
Entity Detected:	Slc7a9, solute carrier family 7 (cationic amino acid transporter, y+ system), member 9 ( MGI:1353656)
Sequence:	sense strand is shown >T56043 TGGTGGTCAGCTGAGAGAGTATCACCGACTCAGACAGAGTCTTTCTATGTACCCCAATTTGGCTTGACTT GCTGGGTAACCCAGGCTGGCCTTGAACTTACTATTTCCTACCTCAGCTACCTCGGTACTGGGAGTACAGC TGACTCAAGAGACTCTCCTCTCAGCCCCTGGAGCTACCAGCGGGCAACCATGGAGGAGACAAGCCTGAGG AGACGGAGAGAGGATGAGAAATCCACCCACAGTACTGAACTCAAGACCACAAGTCTCCAGAAAGAGGTGG GTCTTCTCAGTGGCATCTGTATCATTGTGGGCACCATCATCGGCTCCGGGATCTTCATCTCCCCCAAGTC TGTGCTGGCCAACACAGAATCCGTGGGGCCCTGTCTCATCATATGGGCCGCCTGTGGAATCCTGGCTACA CTGGGCGCCCTGTGCTTTGCAGAGCTTGGCACAATGATTACCAAGTCAGGGGGTGAGTACCCCTATCTGA TGGAGGCCTTTGGCCCCATCCCTGCCTACCTCTTCTCCTGGACCAGCCTGATCGTCATGAAGCCCTCATC CTTCGCCATCATCTGCCTCAGCTTTTCAGAGTATGTGTGTGCAGCCTTTTACTCAGGTTGCAAGCCTCCT GCTGTGGTAGTGAAACTCCTGGCTGCTGCTGCCATCTTGTTTATCACGACAGTGAATGCGCTAAGCGTGC GGCTTGGCAGCTATGTCCAGAATGTCTTCACGGCAGCCAAGATGGTGATCGTGGCCATCATCATCATCAG CGGACTGGTCTTCCTGGCCCAAGGAAATGTAAAGAACTTTCAGAATTCTTTTGAGGGTACACAGACCTCT GTGGGTGCCATCAGTCTGGCATTTTACAATGGACTCTGGGCCTACGATGGTTGGAACCAACTCAACTATA TCACTGAAGAGCTTAGAAACCCTTACAGAAACCTGCCCATGGCCATTGTCATTGGGATTCCTCTGGTGAC TGTATGCTACATCCTCATGAATATTGCCTACTTCACAGTGATGACCCCAACGGAGCTCTTGCAGTCCCAG GCTGTGGCTGTGACCTTCGGAGACCGTGTTCTCTATCCAGCATCTTGGGTAGTCCCACTTTTTGTGGCAT TTTCAACCATCGGTGCTGCTAATGGTACCTGCTTTACAGCCGGCAGGCTCATCTATGTGGCAGGCCGGGA AGGCCACATGCTTAAAGTGCTCTCCTACATCAGTGTCAAGCGCCTCACGCCAGCCCCTGCCCTCATATTT TATGGTATAATAGCCATCATTTACATCATCCCTGGGGACATCAACTCCTTAGTCAATTATTTCAGTTTTG CTGCATGGCTGTTTTATGGTATGACAATCCTAGGACTCGTTGTGATGCGATTCACAAGGAAAGATCTAGA GAGGCCCATCAAGGTGCCCCTCTTCATCCCTATCATTGTGATTCTCGTATCTCTTTTCTTGATCCTGGCT CCAATCATCAGTGAGCCGGCCTGGGAGTATCTCTACTGTGTGTTGTTCATACTGAGTGGACTTATATTTT ACTTCCTTTTTGTCTACTACAAGTTCGGATGGGCCCAGAGAATCTCCAGGCCGGTCACAAAGCACCTGCA GATGCTCATGGAAGTCGTCCCACCAGAAAAGGACCCAGAGTAAAAGGTCCATCACTCGTAGCCCAAAGCT GTGATCAATTTATTTTTGTAAGCAATTATTTGTGGTTATTTCTTCCTGGTGTTTTTCTTAATGAAAACAT GTATTCAGATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4828282 same experiment
	EurExpress:euxassay_019720 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source