EMAGE mouse gene expression spatial database

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EMAGE:11128

Lass2 LAG1 homolog, ceramide synthase 2 ( MGI:1924143)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003624. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003624_01	euxassay_003624_02	euxassay_003624_03	euxassay_003624_04

euxassay_003624_05	euxassay_003624_06	euxassay_003624_07	euxassay_003624_08	euxassay_003624_09

euxassay_003624_10	euxassay_003624_11	euxassay_003624_12	euxassay_003624_13	euxassay_003624_14

euxassay_003624_15	euxassay_003624_16	euxassay_003624_17	euxassay_003624_18	euxassay_003624_19

euxassay_003624_20	euxassay_003624_21	euxassay_003624_22	euxassay_003624_23	euxassay_003624_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11128_wholemount_strong.wlz

11128_wholemount_moderate.wlz

11128_wholemount_weak.wlz

11128_wholemount_possible.wlz

11128_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11128_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6625
Entity Detected:	Lass2, LAG1 homolog, ceramide synthase 2 ( MGI:1924143)
Sequence:	sense strand is shown >T6625 CACCGGCCGGGCGCTAGAAGTGGGAAACGGAGTAGCGGCGCCCGGGCCGGAGCAGCTCTGCACCGGACGC CGAGATGCTCCAGACCTTGTATGACTACTTCTGGTGGGAACGACTATGGCTGCCTGTGAACTTAACCTGG GCTGATCTAGAAGACAAAGATGGACGTGTCTATGCCAAAGCCTCAGATCTCTACATCACACTCCCCCTGG CCCTGCTCTTTCTCGTCATTCGATACTTCTTTGAGCTTTATGTGGCAACACCCCTGGCTGCCCTCCTGAA TGTTAAGGAGAAAACCCGACTGCGGGCACCTCCCAATGCCACCTTGGAACATTTCTACCAGACCAGCGGC AAGCAGCCCAAGCAGGTGGAGGTAGACCTTTTGTCACGGCAGAGTGGGCTCTCTGGACGCCAGGTAGAAC GCTGGTTCCGCCGCCGCCGCAACCAGGACAGGCCCAGTCTTCTCAAAAAGTTCCGAGAAGCCAGCTGGAG ATTCACGTATTACCTAATTGCCTTTGTTGCTGGCATGGCTGTCACTGTGGATAAACCCTGGTTCTATGAC TTAAGGAAAGTTTGGGAGGGATATCCCATACAGAGCATCATCCCTTCTCAGTATTGGTACTACATGATTG AACTTTCCTTCTACTGGTCCCTGCTCTTCAGCATTGCCTCTGATGTCAAGCGAAAGGATTTTAAGGAACA GATCATCCACCATGTGGCCACTATCATTCTCCTCTGCTTCTCCTGGTTTGCCAATTACGTCCGGGCAGGG ACCCTCATCATGGCTCTGCATGACGCTTCTGACTACCTGCTGGAGTCTGCCAAGATGTTTAACTACGCGG GATGGAAGAACACCTGCAACAACCTCTTCATTGTGTTCGCCATCGTTTTCATCATCACTCGGCTGGTTAT CATGCCTTTCTGGATCCTACACTGCACGATGATATACCCACTGGAGCTCTACCCTGCCTTCTTTGGCTAT TATTTCTTCAATTTTATGATGGCAGTGCTACAGATGCTCCATATCTTCTGGGCCTACTTCATTCTGCGTA TGGCCCACAAGTTCATAACTGGAAAGCTGATAGAAGATGAACGCAGTGACAGAGAAGAAACAGAGAGTTC AGAGGGGGAGGAGACTGCAGCTGGGGCAGGAGCAAAGAGCCGGCTCCTAGCTAATGGCCACCCCATCCTC AATAACAATCATCCTAAGAATGACTGAACCATTGTCCTAGCTGCCTCCCACATCAATGCATGAGGCCAAG AAACTAGCCAAGCTTCCCTTTAGGGGCATTTGAGCTCTGGGGAGAAAGGATATGAGGAGTCTACACTGAC CTTCCACTTCTTTTTTCACCCAGTTGCCTTTAAACCAGACTCTAAACAGCCTATCTCCAGGTAGAAAGGA GGCTTGGCCACATCCACATCCTGTTGAGAGACATAGTCTCATTTCCCTCTCTACCTTCTAAGCTACCCTT CTTGCTAATCCTTCTTCAGCTCTGAGGGTTGGAATCATAACTCACATTCCTCATTCCTAAGAATTTGGAG CTAGCTGTTTGCCTTTGACTCCCTGACTTCAAGCCAGGGTTGTGCCTTACTGTCCCTTTTCTGGGCCTCA TTCTGCCAAAGCTGGACCAAGGTTCCATCTTTCTAAGCTCCCTAGCTCAGGCCAAGAAAGAAAGCTGAGC TACTTTTAACTTTCTCTTCCTTAAATACAAGTCAATGAAGTGAAGGAGGGTATGCATACATGCCCCTCAC CCTACCCCAGGGACCATGGTTCCTAGGATCTCACTGCCTCCCTCTCTGGCCTTCCTGTCCCCTCCCTTCA GCTATGACAGCTGGTGTGGAGTAGAAGGGCAACTAGTTCTGTTATTTATTGAACATTTGGGGTTTCAGTT GTAAAGCCACAACTACAGGTAGGACCTGATATTTCGGCGAGGGACCATTTCAGACCAAAATGTACTGTTA ATTTTTTTTAATTAAAGTATATTAAAGGTTAAATAAAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3598473 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3598473 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11132 same embryo
	EMAGE:11131 same embryo
	EMAGE:11129 same embryo
	EMAGE:11133 same embryo
	EMAGE:11130 same embryo
	EurExpress:euxassay_003624 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source