EMAGE mouse gene expression spatial database

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EMAGE:11130

Senp8 SUMO/sentrin specific peptidase 8 ( MGI:1918849)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003630. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003630_01	euxassay_003630_02	euxassay_003630_03	euxassay_003630_04

euxassay_003630_05	euxassay_003630_06	euxassay_003630_07	euxassay_003630_08	euxassay_003630_09

euxassay_003630_10	euxassay_003630_11	euxassay_003630_12	euxassay_003630_13	euxassay_003630_14

euxassay_003630_15	euxassay_003630_16	euxassay_003630_17	euxassay_003630_18	euxassay_003630_19

euxassay_003630_20	euxassay_003630_21	euxassay_003630_22	euxassay_003630_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11130_wholemount_strong.wlz

11130_wholemount_moderate.wlz

11130_wholemount_weak.wlz

11130_wholemount_possible.wlz

11130_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11130_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

testis

moderate

regional

moderate expression: see section 05 06 weak expression: see section 07 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6628
Entity Detected:	Senp8, SUMO/sentrin specific peptidase 8 ( MGI:1918849)
Sequence:	sense strand is shown >T6628 CCACGCGTCCGAGGCTGGTTGGGTTGGTGAGGCCTGAGGAATAGCTTCTGGAATTTCTGAGAATCCCTGC TCAATACAAGATGGACCCTGTGGTCTTGAGTTATATGGACAGTCTATTGCGGCAATCAGATGTCTCACTA TTGGACCCTCCAAGCTGGCTCAACGACCACATTATTGGGTTTGCCTTTGAATACTTTGCCAATAGTCAGT TTCATGACTGCTCAGACCATGTCTGTTTCATCAGCCCTGAAGTCACCCAGTTCATTAAGTGCACCAGCAG CCCTGCAGAAATTGCCATGTTCCTTGAACCCCTGGATCTTCCCCACAAAAGAGTGGTATTTTTAGCCATC AATGATAATTCTAACCAGGCAGCCGGAGGTACCCACTGGAGTTTGTTGGTTTATCTCCAAGATAAAAATA GCTTCTTTCATTATGATTCCCATAGCAGGAGCAACTCAATCCATGCAAAGCAGGTAGCAGAGAAACTGAA GGCTTTCTTAGGCAGCAAAGGAGACAAACTGGTCTTTGTGGAAGAGAAAGCCCCAGCTCAAGAGAACAGC TATGACTGTGGGATGTACGTGATATGTAACACCGAGGCCTTGTGTCAGAGTCTCTTTAGACGGCAGCCAG AGTCCCCGCTGCAGCTACTCACCCCGACATACATCACAAAGAAGAGAGGAGAATGGAAAGATCTAATTGC CAGACTTGCTAAAAAAAACGAAGTAGCTACTGAAGAATGCTCGTGATGTTTGAAGTCTGGTCTTTCTGCC TTTCTCTATGGTGACAGCCTCAGTGCCTGAGGGAAAACCACTAGTAGATGAAAGTTTACAATTCCTGCTT CTTTATTGTTCTGTAAGAATGTCATCCTCTGTGTTCTCTGAATGGGAAGTTTCACTGTAATCCTGAGGAA AGCGGTACATTCTGTGGAAATGTCTTGAACTTACGTACCAAAACTTTAGTAACAAACCTCTCATTACACA GTTTCAAGTAAGAGCCTTCTTTTCTTGTTGGTATAAAACTATTATCCCTTTTGGTCTCCCTTCCTTGTTG GCTTATTCCAAAGAAGTGATGTTCCAAAGCAAACCAATAACATGAAGTCTAAAGACATGCATGAAGAAAA CAGCAGAAGAACCAACATCTTTGTTTCACAGCCATAGTGAGGCGTCCGACTGGAGGTGGATCATTACCTA ACTCCACTTATAGCCTTCGGTTTATAAAAATAAGGCATTCTCGGGTGCAAAGGCTTTCCCGTCTGCAAAT ACTTAATAAAGATGACTTTAGCTCATCTTTATCTATTTCCTGGAATGCTTTAAGAACATGGAACGTCATT AAAATAAATGGCTATAGACTGGGAGCATAACTTTGAGGAAGAGCACCTGCTGTGGTGTGCAGGAAGACTT GAACTCAATTCCAAATTGCAGTAAATAAAAACAAAACAAAACCACCTGCGGGTGTGTGGTGCATCTCTGG TTTCCCTGAGTGAGTGGGTGACTCAGCTCTAAGTGATGGTGACTTTGTCTTGTCCTTTACATGGATTTAG TCCCTCCCTTTACCTAACGTGCTGTAGTTCTCAAGCTCACAGGATAACCAGCCAAGATTAGCTGTTATTT CAGACATGTTCTACTTTTGGTAGATAGAGCTTTTACACTAATCTAGCAGAGTTGTTTTCAGGTATGTTGT GGTAATCTCCATACTATTAATGATCTAAAACATAGGAGCAGTTTATTTTTTAAATATAAAGGAAAAAAAA TGTCACCTTCCTCAAACTTAATCCAAAAAGTAAATGGTATAACTTTAAAATATGGAAACAAGACTAAAAT GAAATACTACTCCCCACAAAGCTATTGATAAATTTTTCTACTTGTTTCAAGTAATTAACACAAGAAGTTG GGCACACATAATAATTAGACCCCGATAACAATTTGCAGTGTATATGGGATGTAAAACACAAAGATGTGAT TCACTCACTGAAACTGACTTCAGCTACTTCTCCACTCTAACCCTGACCCCAGCGTTCATAACCCAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3599077 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3599077 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11132 same embryo
	EMAGE:11131 same embryo
	EMAGE:11129 same embryo
	EMAGE:11128 same embryo
	EMAGE:11133 same embryo
	EurExpress:euxassay_003630 same experiment
	MGI:4827954 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source