EMAGE mouse gene expression spatial database

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EMAGE:11134

Sh3bgrl3 SH3 domain binding glutamic acid-rich protein-like 3 ( MGI:1920973)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003517. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003517_01	euxassay_003517_02	euxassay_003517_03	euxassay_003517_04

euxassay_003517_05	euxassay_003517_06	euxassay_003517_07	euxassay_003517_08	euxassay_003517_09

euxassay_003517_10	euxassay_003517_11	euxassay_003517_12	euxassay_003517_13	euxassay_003517_14

euxassay_003517_15	euxassay_003517_16	euxassay_003517_17	euxassay_003517_18	euxassay_003517_19

euxassay_003517_20	euxassay_003517_21	euxassay_003517_22	euxassay_003517_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11134_wholemount_strong.wlz

11134_wholemount_moderate.wlz

11134_wholemount_weak.wlz

11134_wholemount_possible.wlz

11134_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11134_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15

medulla oblongata basal plate

moderate

regional

moderate expression: see section 07 08 09 10 12 14 15 16

metencephalon basal plate

moderate

regional

moderate expression: see section 06 16

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 19 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 07 17

ventral grey horn

moderate

regional

moderate expression: see section 09 14 15 weak expression: see section 10 11 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5918
Entity Detected:	Sh3bgrl3, SH3 domain binding glutamic acid-rich protein-like 3 ( MGI:1920973)
Sequence:	sense strand is shown >T5918 CTCCCAAGCGCGGCGGCGGCAGCGTAGTTCCTAGCCGTGCAGCTGCCGCTCCCACCGTCCCTCAGTTGGT CTGTGTCCCCCGCCTGCAGCATGAGTGGCCTGCGCGTCTACAGCACGTCGGTCACCGGCTCCCGAGAAAT CAAGTCCCAGCAGAGTGAGGTGACCCGCATCCTAGATGGGAAGAGGATCCAATACCAGCTAGTGGACATC TCCCAGGACAACGCCCTTCGAGATGAGATGCGAACCTTGGCTGGCAACCCCAAAGCTACCCCACCTCAGA TTGTCAACGGGAACCACTACTGTGGGGACTATGAACTCTTCGTGGAGGCCGTGGAGCAGGACACACTGCA GGAGTTCCTGAAGCTGGCCTGAGTCAGGATGGCCACCGATCCTATCCAGCTAGCTCTTCACCAGGCCTTG TAACCAACTCCTTGTCGTCCTAATCTGCAAACCCAGACCCAGACCCTTTCCGCAGCCGCCTCCTCTTCCT CCAGAGGCAACATTCCTTATTCACCAACTAGTCTCAAAAGATTGTCTTAAGCCCTGACGATGACTGTCCC CAGCCACACCTTGGGGCATCCTCTCTGCCTGGCCCTGTCTGATGAGCATTTCTGTTGCTTCCATCAGCCA GAGCTCTGCCAAAGGCCCCGCATCCCCATCCTAGGAGTGCCCTAGAGACAATTAAATGTCCATTCCTCTG GTGCTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3481987 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3481987 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11139 same embryo
	EMAGE:11138 same embryo
	EMAGE:11137 same embryo
	EMAGE:11136 same embryo
	EMAGE:11135 same embryo
	EurExpress:euxassay_003517 same experiment
	MGI:4828035 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source