EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11135

Imp4 IMP4, U3 small nucleolar ribonucleoprotein, homolog (yeast) ( MGI:106572)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003515. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003515_01	euxassay_003515_02	euxassay_003515_03	euxassay_003515_04

euxassay_003515_05	euxassay_003515_06	euxassay_003515_07	euxassay_003515_08	euxassay_003515_09

euxassay_003515_10	euxassay_003515_11	euxassay_003515_12	euxassay_003515_13	euxassay_003515_14

euxassay_003515_15	euxassay_003515_16	euxassay_003515_17	euxassay_003515_18	euxassay_003515_19

euxassay_003515_20	euxassay_003515_21	euxassay_003515_22	euxassay_003515_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11135_wholemount_strong.wlz

11135_wholemount_moderate.wlz

11135_wholemount_weak.wlz

11135_wholemount_possible.wlz

11135_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11135_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 21 22

thymus primordium

moderate

homogeneous

moderate expression: see section 12 13 14 15 16

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 11 17 18 19 20

vibrissa

moderate

regional

moderate expression: see section 07 08 09 10 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5912
Entity Detected:	Imp4, IMP4, U3 small nucleolar ribonucleoprotein, homolog (yeast) ( MGI:106572)
Sequence:	sense strand is shown >T5912 CTCATCATGCTCCGTCGGGAGGCTCGCCTGCGCCGCGAGTACCTGTACCGCAAAGCCCGGGAGGAGGCGC AGCGATCCGTTCAGGAGAAGAAGGAGCGAGTCAAGCGTGCTCTAGAAGAAAACCAGCTGATTCCCACCGA GTTACGCCGGGAGGCTCTAGCCTTACAGGGGTCCTTGGAGTTCGATGATGCTGGTGGAGAAGGTGTAACC AGCCATGTAGATGATGAATATCGATGGGCCGGGGTTGAGGATCCTAAGGTCATGATCACTACCTCCAGAG ACCCTAGTTCTCGCCTGAAAATGTTTGCAAAGGAACTGAAGCTGGTGTTCCCAGGTGCCCAGCGCATGAA CCGAGGTCGGCATGAGGTTGGAGCACTAGTGAGAGCTTGCAAAGCCAACGGGGTCACCGACCTGTTGGTG GTCCATGAGCATCGAGGCACACCCGTGGGGCTCATTGTCAGCCATCTGCCCTTCGGTCCTACTGCCTATT TCACACTGTGTAACGTGGTCATGCGGCACGACATCCCTGACCTGGGCACCATGTCAGAAGCCAAGCCCCA CCTCATCACTCATGGCTTTTCCTCACGGCTGGGCAAGCGGGTGTCGGACATCCTTCGTTACCTATTCCCC GTGCCAAAAGACGACAGCCATCGGGTCATCACCTTTGCAAACCAGGATGACTACATTTCATTCCGGCACC ATGTCTACAAGAAGACAGACCACCGCAATGTGGAGCTGACTGAGGTCGGGCCTCGATTTGAGCTCAAGTT GTATATGATTCGCCTGGGCACACTGGAGCAGGAGGCCACCGCAGATGTAGAGTGGCGCTGGCACCCCTAC ACCAACACCGCCCGCAAGAGGGTCTTCCTGAGTGCCGAGTGAGCTGAGCTACTTGCCAGTCAGAATGCAG ACTTGGAATCCTGGATCAGAGACTTGTCACAGACAGATTCACTAATCCTAAGTGGAGACTGGAGGGATCT AAATAACCCCTCTGTCATCACGCTCCCAGCTGCCTCTAACTGGTCACGGTTGGCCTACTGTCTGTCAGGA CAAACCTGAAGCCCACAGAGTATCTATGGGTTGAGGACCTAATGACAGTGGACTGAATAGTCACCTTCAG GCTGGGGTAGGGCTGCAGATCTGCACTGGAAAGTGGTTACTTTCTTCAGAGTTGGGCCCTGTGAACACTT TTTTCCTGTGGTTTAGGGTCAGTCTTTGTCACCAGCAGCAAGCCTACACCATTTAATTACCAGCCTAACT TACAAGCAGGGGCACCCTCCTCTAACTGCGAGCTTTTCCCCTCAGCCTCTTTGTTCGAAGAGATAGCGAC TCTAAGACTTAATATTTCATGAATAAACGCCTAGGCCAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3481801 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3481801 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11139 same embryo
	EMAGE:11138 same embryo
	EMAGE:11137 same embryo
	EMAGE:11136 same embryo
	EMAGE:11134 same embryo
	EurExpress:euxassay_003515 same experiment
	MGI:4825587 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source