EMAGE mouse gene expression spatial database

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EMAGE:11137

Ceacam10 carcinoembryonic antigen-related cell adhesion molecule 10 ( MGI:1347248)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003439. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003439_01	euxassay_003439_02	euxassay_003439_03	euxassay_003439_04

euxassay_003439_05	euxassay_003439_06	euxassay_003439_07	euxassay_003439_08	euxassay_003439_09

euxassay_003439_10	euxassay_003439_11	euxassay_003439_12	euxassay_003439_13	euxassay_003439_14

euxassay_003439_15	euxassay_003439_16	euxassay_003439_17	euxassay_003439_18	euxassay_003439_19

euxassay_003439_20	euxassay_003439_21	euxassay_003439_22	euxassay_003439_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11137_wholemount_strong.wlz

11137_wholemount_moderate.wlz

11137_wholemount_weak.wlz

11137_wholemount_possible.wlz

11137_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11137_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 06 07 08 09 16 17 18 19 20 moderate expression: see section 05

hindgut

strong

regional

strong expression: see section 11

rectum

strong

regional

strong expression: see section 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5917
Entity Detected:	Ceacam10, carcinoembryonic antigen-related cell adhesion molecule 10 ( MGI:1347248)
Sequence:	sense strand is shown >T5917 CCCACGCGTCCGCCCACGCGTCCGGGCAGATACCTGGGCAGCCAGGCTTAGCAGTAGTGTTGGAGAAGAA GCTAGCAGGCAGGCAGCAGAGACATGGAGCTGGCCTCAGCACATCTCCACAAAGGGCAGGTTCCCTGGGT TGGACTACTGCTCACAGCCTCACTTTTAACTTACTGGAGCCCTGCCACTACTGCCCAAGTCACCGTTGAG GCTGTGCCGCCCAACGTTACTGCAGACAACAATGTTCTTCTACTTGTTCACAATCTGCCGCAGACGCTCC GAGTCTTTTACTGGTACAAGGGAAACAGTGGGGCTGGACACAATGAAATTGGACGATTTGTAACATCCAT TAATAGGAGTAAAATGGGGCTTGCACACAGCGGCAGAGAGACAATATATAGCAATGGATCCCTGTTCTTC CAAAGTGTCACCAAGAATGATGAGGGAGTCTACACATTATATATGCTAGATCAAAACTTTGAAATTACTC CGATTTCTGTGCGATTTCATGTACACCCCTCACTTTTACCCTCCTTGAGCCCTCCCACCACTGGCCAAGT CACCGTTGAGGCTGTGCCGCCCAACGTTGCCGAAGGGGAAAACGTTCTTCTACTTGTTCACAATCTGCCA CGGACGCTCCGAGCAATTTACTGGTACAGGGGAACCACTGCGGGTGAAAGAAATGAAATTACACGATTTA TAACAGCCTCCAATAAGATTATATTGGGGCCTGCACACAGCGACAGAGAGATCATATACAACAATGGATC CCTGTTCTTCCAAGGTGTCACCAAGAATGATGAGGGAGCCTACGCACTAGATATGCTATTTCAAAACTTC GATCATACCCTAATGCCTGTGCAATTTAATGTTCACGCTAAAAAGCAGTAGGAATTTTTTTTCCAGCCCC CTTGCATTTAATTCTTCTTTCATTGCTCTTTCTAACTGGAACCGAACTCTGGGAAAGAGGGCTGATGCTC ACTAGTGAACCCCAGGGATTTCATCAGCTGACGGTGCCTTGCTTCACCTCCTTCCACCAGCAATAAAGTC CGCATAACTTCTGCAAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3481822 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3481822 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11139 same embryo
	EMAGE:11138 same embryo
	EMAGE:11136 same embryo
	EMAGE:11135 same embryo
	EMAGE:11134 same embryo
	EurExpress:euxassay_003439 same experiment
	MGI:4823795 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source