EMAGE mouse gene expression spatial database

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EMAGE:11146

Polr3f polymerase (RNA) III (DNA directed) polypeptide F ( MGI:1924086)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003491. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003491_01	euxassay_003491_02	euxassay_003491_03	euxassay_003491_04

euxassay_003491_05	euxassay_003491_06	euxassay_003491_07	euxassay_003491_08	euxassay_003491_09

euxassay_003491_10	euxassay_003491_11	euxassay_003491_12	euxassay_003491_13	euxassay_003491_14

euxassay_003491_15	euxassay_003491_16	euxassay_003491_17	euxassay_003491_18	euxassay_003491_19

euxassay_003491_20	euxassay_003491_21	euxassay_003491_22	euxassay_003491_23	euxassay_003491_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11146_wholemount_strong.wlz

11146_wholemount_moderate.wlz

11146_wholemount_weak.wlz

11146_wholemount_possible.wlz

11146_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11146_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

testis

moderate

regional

moderate expression: see section 05 06 07 08 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6691
Entity Detected:	Polr3f, polymerase (RNA) III (DNA directed) polypeptide F ( MGI:1924086)
Sequence:	sense strand is shown >T6691 CTCGCCTCTCTCTCAAGCCTCGGTCCCCGGCGCGCGCCTCCACCGGTTCCCGAGACTTCCAGCGCGCTCC GAGGGGCCACTTTTCCTCTAGTCTCGAAAACTGCCCTCATGGCGGAGGTGAAGGTGAAGGTGCAGCCGCC GGACGCGGATCCGGTGGAGATAGAAAACAGAATTATAGAATTATGCCACCAGTTCCCTCATGGAATCACA GATCAAGTAATTCAGAACGAAATGCCTCACATTGAAGCCCAGCAGCGGGCAGTGGCCATCAATAGACTGT TATCCATGGGTCAGTTGGATCTTTTAAGGAGTAATACAGGCCTCTTATATAGAATAAAGGATTCTCAGAA TGCTGGTAAAATGAAGGGGTCCGATAACCAGGAAAAACTGGTCTATCAAATCATAGAGGATGCAGGGAAT AAAGGAATATGGAGCAGAGATATTCGGTACAAAAGTAACTTGCCGTTAACAGAAATCAACAAAATTCTCA AGAATTTGGAAAGTAAAAAGCTAATTAAAGCCGTGAAGTCTGTAGCAGCTTCCAAAAAGAAGGTTTATAT GCTCTATAACCTGCAGCCAGACCGATCTGTGACTGGCGGTGCCTGGTACAGTGACCAGGATTTTGAATCT GAATTTGTTGAGGTGCTTAATCAACAGTGTTTCAAATTCCTACAAAGCAAGGCAGAGACAGCAAGAGAAA GTAAACAGAATCCAGTGATACAAAGAAATAGTTCATTTGCATCGTCACATGAAGTCTGGAAGTACATCTG TGAGCTGGGCATCAGCAAGGTAGAGTTGTCCATGGAGGACATTGAAACCATCTTGAACACACTCATTTAT GATGGCAAAGTGGAGATGACTATCATTGCTGCGAAGGAAGGCACTGTTGGCAGCGTAGATGGACACATGA AACTGTACAGGGCAGTCAACCCAATCCTCCCACCTACAGGCGTGGTCCGGGCACCCTGTGGACTCTGCCC CGTTTTTGAGGACTGCCATGAAGGTGGTGAGATTTCGCCGTCTAATTGTATTTACATGACCGAGTGGCTT GAATTTTAATGAGAGCTGTGCACTTTATCTACATTTGGCAAACTGAACTCCATGGAGAACAAACAATTCA TGATGGAGTGTAAATTTCTTTGAAAGCATACTTCACAATAATGGATACTGACTTGCTGCTATGAAAACAT TTATTTATGAAGACAGAACACAAAGCAGATTGAGTTTAATAAAGTTTAATTGGGAAGTCTCTTCTTCAGT AAGTAAAATACCTTGAAGGTCTGGAAGGCAAGGAAAAGAAGCCAGCAAGCCTATGTTGACCAAGTACTGA GATAAAGGCTGGAACGAGAAGATGTTCACTAGAGTTGCTTCATTGAAAACTCATCGGGGTTATTACCACC ATTTGTTTGGTTGTTTTTGGGTTTTGTTGAGAAGAAATTACAAGTGGTGCAGATAGGAACAGCTGATGGC ATGTGTGCCATCTTGGGGCCTGGGTAGTCAAGTGGGCATTCCTTTTTCATGCCATCTGTTGCTCTTCACC CCAGGACTCTTTCAGGGGAGTGTGCCTCCATACCGACCTCACTCCCAAATGCTGTTGTACAGGACGTGTT TCCACATCACTGTTATTCCATGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3602180 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3602180 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11149 same embryo
	EMAGE:11151 same embryo
	EMAGE:11147 same embryo
	EMAGE:11148 same embryo
	EMAGE:11150 same embryo
	EurExpress:euxassay_003491 same experiment
	MGI:4827326 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source