EMAGE mouse gene expression spatial database

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EMAGE:11148

Fyn Fyn proto-oncogene ( MGI:95602)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003478. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003478_01	euxassay_003478_02	euxassay_003478_03	euxassay_003478_04

euxassay_003478_05	euxassay_003478_06	euxassay_003478_07	euxassay_003478_08	euxassay_003478_09

euxassay_003478_10	euxassay_003478_11	euxassay_003478_12	euxassay_003478_13	euxassay_003478_14

euxassay_003478_15	euxassay_003478_16	euxassay_003478_17	euxassay_003478_18	euxassay_003478_19

euxassay_003478_20	euxassay_003478_21	euxassay_003478_22	euxassay_003478_23	euxassay_003478_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11148_wholemount_strong.wlz

11148_wholemount_moderate.wlz

11148_wholemount_weak.wlz

11148_wholemount_possible.wlz

11148_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11148_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6696
Entity Detected:	Fyn, Fyn proto-oncogene ( MGI:95602)
Sequence:	sense strand is shown >T6696 CCACGCGTCCGAAACAAAGCAAAGGAACAGGTGGTGGGGAGTTTGCTGCATGGATGGGTGCCCCAATCGA TCGATCGAGTCAGTCCGACCGCTGGTTTCGAAGACATGTGGTGTATATAAAGTTTGTGATAGTTGGTGGA AATTTGGGAGCTTGGATAATGGGCTGTGTGCAATGTAAGGATAAAGAAGCAGCGAAACTGACAGAGGAGA GGGACGGCAGCCTGAACCAGAGCTCTGGGTACCGCTATGGCACAGACCCCACCCCTCAGCACTACCCCAG CTTCGGCGTGACCTCCATCCCGAACTACAACAACTTCCACGCAGCTGGGGGCCAGGGACTCACCGTCTTT GGGGGTGTGAACTCCTCCTCTCACACTGGGACCCTACGCACGAGAGGAGGGACAGGAGTGACACTGTTTG TGGCGCTTTATGACTATGAAGCACGGACGGAAGATGACCTGAGTTTTCACAAAGGAGAAAAATTTCAAAT ATTGAACAGCTCGGAAGGAGACTGGTGGGAAGCCCGCTCCTTGACAACCGGGGAAACTGGTTACATTCCC AGCAATTACGTGGCTCCAGTTGACTCCATCCAGGCAGAAGAGTGGTACTTTGGAAAACTTGGCCGCAAAG ATGCTGAGAGACAGCTCCTGTCCTTTGGAAACCCAAGAGGTACCTTTCTTATCCGCGAGAGCGAAACCAC CAAAGGTGCCTACTCACTTTCCATCCGTGATTGGGATGATATGAAAGGGGACCACGTCAAACATTATAAA ATCCGCAAGCTTGACAATGGTGGATACTATATCACAACGCGGGCCCAGTTTGAAACACTTCAGCAACTGG TACAGCATTACTCAGAGAAAGCTGATGGTTTGTGTTTTAACTTAACTGTGGTTTCATCAAGTTGTACCCC ACAAACTTCTGGATTGGCTAAAGATGCTTGGGAAGTTGCACGTGACTCGTTGTTTCTGGAGAAGAAGCTG GGGCAGGGGTGTTTCGCTGAAGTGTGGCTTGGTACCTGGAATGGAAATACAAAAGTAGCCATAAAGACCC TTAAGCCAGGCACCATGTCTCCGGAGTCCTTCCTGGAGGAGGCGCAGATCATGAAGAAGCTGAAGCATGA CAAGCTGGTGCAGCTCTACGCGGTCGTGTCTGAGGAGCCCATTTACATCGTCACGGAGTACATGAGCAAA GGAAGTTTGCTTGACTTCTTAAAAGATGGTGAAGGAAGAGCTCTGAAGTTGCCAAACCTTGTGGACATGG CGGCACAGGTTGCTGCAGGAATGGCTTACATCGAGCGCATGAATTATATCCACAGAGATCTGCGATCAGC AAACATTCTAGTGGGGAATGGACTAATTTGCAAGATTGCTGACTTTGGATTGGCTCGGTTGATTGAAGAC AATGAATACACAGCAAGACAAGGTGCGAAGTTTCCCATTAAGTGGACAGCCCCCGAAGCGGCCCTGTATG GAAGGTTCACAATCAAGTCTGACGTATGGTCTTTTGGAATCTTACTCACAGAGCTGGTCACCAAAGGAAG AGTGCCATACCCAGGCATGAACAACCGGGAGGTGCTGGAGCAGGTGGAGAGAGGCTATAGGATGCCCTGC CCACAGGACTGCCCGATCTCCCTGCACGAGCTCATGATCCACTGCTGGAAAAAGGATCCGGAAGAGCGCC CGACCTTCGAGTACTTGCAGGGCTTCCTGGAGGACTACTTTACGGCCACAGAGCCCCAGTATCAGCCCGG TGAAAACCTGTGAGAGCCTGCGCTTCAGACGCCTCTTCCCGAGGCCTCCCTACCCCTCCCCATTAGCTTC CAATTCTGTAGCCAGCTGCCCCAGAGCAGCGAGAACCGTCCAGGATCAGATTGCATGTGACTCTGAAGCT GAACTTCCACGGCCCTCATTAATGACACTTGTCCCCCAGTCCGAACCTCCTCTGTGAACCATCTGAGACA GAAGCGTGTTATTTCTCAGACTTGGAAATGCATTGTATCGATGTTATGTCAAAGGCCAAACCTCTGTTCA GTGTAAATAGCTGCTCCTGTGCCAACAATCCCAGTGCTTTCCTTTTTTAAAAAAGAAAAAGCAAATCCTA TGTGATTTTAACTCTGTCTTCACCTGATTCAACTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3602546 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3602546 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11149 same embryo
	EMAGE:11151 same embryo
	EMAGE:11147 same embryo
	EMAGE:11150 same embryo
	EMAGE:11146 same embryo
	EurExpress:euxassay_003478 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source