EMAGE mouse gene expression spatial database

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EMAGE:11149

Mettl13 methyltransferase like 13 ( MGI:1918699)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003461. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003461_01	euxassay_003461_02	euxassay_003461_03	euxassay_003461_04

euxassay_003461_05	euxassay_003461_06	euxassay_003461_07	euxassay_003461_08	euxassay_003461_09

euxassay_003461_10	euxassay_003461_11	euxassay_003461_12	euxassay_003461_13	euxassay_003461_14

euxassay_003461_15	euxassay_003461_16	euxassay_003461_17	euxassay_003461_18	euxassay_003461_19

euxassay_003461_20	euxassay_003461_21	euxassay_003461_22	euxassay_003461_23	euxassay_003461_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11149_wholemount_strong.wlz

11149_wholemount_moderate.wlz

11149_wholemount_weak.wlz

11149_wholemount_possible.wlz

11149_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11149_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 04 05 06 07 08 21 22 23 24

submandibular gland primordium

moderate

regional

moderate expression: see section 21 weak expression: see section 08 09 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6692
Entity Detected:	Mettl13, methyltransferase like 13 ( MGI:1918699)
Sequence:	sense strand is shown >T6692 CTAAGATTCCCGCCGCTGCTGCCTCCCGGTGCTTCCCGGGGTGATCTGTCACCAGGAGCAGTGTCGAAGA CGCCGCTCACGGTGGGAACATGAATCTCCTACCTAAAAGCTCCAAGGAGTTTGGCTCTGCCGACTACTGG GAGAAGTTCTTCCAGCAGCGAGGGAAGACAGCTTTCGAGTGGTATGGGACCTATCTGGAGCTGTGCGAGG TGCTGCACAAATACATAAAGCCCAAGGAAAAGGTGCTTGTGATCGGATGTGGCAACTCAGAGCTCAGTGA ACAACTCTATGATGTGGGCTATCAGGATATCGTGAACATCGACATCAGCGAGGTTGTCATCAAGCAGATG AAGGAACGCAATGGCAGCCGACGGCCCCACATGAGTTTCCTGAAGATGGACATGACCCAGCTGGAGTTTC CTGACGCCACATTCCAGGTGGTGTTGGACAAGGGCACGCTGGATGCTGTCCTGACAGATGAGGAGGAGGT GACCCTGCGTCAGGTGGACAGGATGCTAGCTGAGGTTGGCCGTGTCCTGCAGGTGGGCGGTCGCTACCTC TGCATCTCCCTGGCTCAGGCTCACATCCTGAAGAAAGCAGTGGGCCACTTCTCTCGGGAGGGGTGGATGG TGAGGGCACACCAAGTGGCCAGCAGCCAGGACCGGGTGTCTGAAGCCGAGCCCCGCTTCTCCCTGCCCGT CTTTGCCTTCGTCATGACTAAGTTCAGGCCAGTCCCTGGCTCTGCCCTTCAGATCTTTGAGCTGTGTACT CAGGAGCAGGGCAAGCCCGTAAGGCTGGAGAGTGCTGATCAGCTGGCGGAGGCTGTGCGGGAGCGCCAGT ACTATGCTTGGCTGTGTAGTCAGCTGCGCCGCAAAGCCGGGCTGGGGAGTGTGTCCCTGGACTTGTGCAG TGGGGACACGGGGGAGCCACGCTACACCCTCCACGTGGTGGACAACCCCGCTGTGAAACCATCGCGGGAC AATCATTTTGCCATTTTCATCATCCCCCAGGGTCGCGAGACCGAGTGGCTCTTTGGCATGGAGGAGGGCC GGAAGCAGCTGGCAGCCAGTGCGGGCTTCAGGAGGTTGGTCACAGTGGCTCTCCACCGAGGCCAGCGGTA TGCTGGCATGGAAAGCATTCAAGCAGAGTTGTCAGCCAGAGTCATGGAGCTGGCCCCGGCTGGGTTGCCT CCCCAGCAGCAGGTGCCCTTCCTGTCTGTGGGTGGGGACATTGGGGTCCGGACTGTGCAGCACCAAGACC ACAGTGCCTTGAGTGGTGACTATGTTATTGAGGATGTGCAAGGGGAAGACAGATGGTACTTTCGGCGGCT CATCTTCCTCAGCAACAGGAACGTGGTGCAGTCGGAAGCCAGGCTGCTGAAGGATACGTCTCACAGAGCC CAGAAGAAACGGAAAAAGGACAGGAAAAAACAGCGACCTGCTGATACTTCTGAGGACTTCCCGCCTGCCC CAGGGCAGTCCATCGATAAGAGTTACCTGTGCTGTGAACACCATAAAGCCATGGTTGCTGGCCTTGCCCT GCTGAGAAACCCCGAGCTACTCTTAGAGACTCCACTGACATTGCTGGTGGTGGGTCTGGGTGGAGGTAGC CTCCCTCTCTTCGTTCACGATCATTTCCCGAAGTCCCGCATCGATGCTGTTGAGATTGACCCCACAATGT TGGAAGTGGCCACTCAGTGGTTTGGCTTCTCACAGAGCGACCGGATGAAGGTTCACATTGCAGATGGTCT TGACTACATTACCAGCCTGGCAGGAGAAGCGCCGCCTCACTATGATGTGATAATGTTTGATGTAGACAGT AAGGATCCGACGCTGGGGATGAGCTGTCCTCCCCCAGCATTTGTGGACCAAGTTTTCCTGCAGAAGGTCA AAAGCATCCTGTGTCACGACGGCGTCTTCATCCTTAACCTCGTGTGTCGGGACGTGAGGTTAAAGGACTC AGTGCTGGCTGGACTCAAGGCAGCCTTCCCTCTCCTCTATGTCCGGCGGATTGAAGGCGAGGTGAATGAA ATCCTGTTTTGTCAGCTGCATCCAGAACAGAAACTTGCTACACCAGAGCTCCTGGAAATGGCCCAGGTTT TGGAGCGGACCCTGCGGAAGCCTGGGCAGGGTTGGGATGACACGTATGTTCTGTCAGATATGCTGAAGAC TGTGAAGATTGTGTGAGCCCTGGGGCCAGGCAACTCAGCTTTCTGTCCAAACTGACTTGAACTCCTGGGC TGCTGGGAGCTAAAGCATGACGCACAGGACTTTTAGACCTCATGCTTTTGTTGGTCACATAATCAGCTAC ATGCTTCCCACCTTTTGGGATGGGAGGCAGCATGGGGGTGATGATGAAAGCCGGGTGGTGGTAGCACAGG CCTTAATCCCGGCACTTGAGAGGCAGAGGCAAATGGATCTTTATAAGTTCAAGGCCAGTCTGAACTTACA AAGTGAGTTCAGGACAGCCAAGGCTACACAGATATTTAATGTCTTCTCAAAGGTCATATGTTGAAAGCTG GCTCCCCAGCCCCCATGGCTCCTTTGGATGATGGTAGAACTTGGGAGGAACTTTGTAGAAGGAAATTGGG TCACTGGTGTGCCCTTGAAGGGCCCTTGGGACACTGGCCCGCCTCTTTGCCTTTTGGCTGCTGTCAAGTG AAGAACTCATCCCCTCATATCTTCCCCTTCATGATGCCCTGCCTTACCACAGGCACAAGGACAGTGACTG GGTCTGGTCACTGCTAGCCTGGAACCTCCACACTAAGCCAAATAAACTTTCTTTCTTGAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:3602469 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3602469 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11151 same embryo
	EMAGE:11147 same embryo
	EMAGE:11148 same embryo
	EMAGE:11150 same embryo
	EMAGE:11146 same embryo
	EurExpress:euxassay_003461 same experiment
	MGI:4826170 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source