EMAGE mouse gene expression spatial database

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EMAGE:11150

Ggt7 gamma-glutamyltransferase 7 ( MGI:1913385)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003479. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003479_01	euxassay_003479_02	euxassay_003479_03	euxassay_003479_04

euxassay_003479_05	euxassay_003479_06	euxassay_003479_07	euxassay_003479_08	euxassay_003479_09

euxassay_003479_10	euxassay_003479_11	euxassay_003479_12	euxassay_003479_13	euxassay_003479_14

euxassay_003479_15	euxassay_003479_16	euxassay_003479_17	euxassay_003479_18	euxassay_003479_19

euxassay_003479_20	euxassay_003479_21	euxassay_003479_22	euxassay_003479_23	euxassay_003479_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11150_wholemount_strong.wlz

11150_wholemount_moderate.wlz

11150_wholemount_weak.wlz

11150_wholemount_possible.wlz

11150_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11150_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forebrain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 07 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 19 20

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 18 19 20 21 22 23 24

vagus x ganglion

strong

regional

strong expression: see section 09 19 not examined expression: see section 10

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 09 18 19 20 21

spinal cord

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 17 18

cervical ganglion

moderate

regional

moderate expression: see section 10 18

thoracic ganglion

moderate

regional

moderate expression: see section 12 13 14 16

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 15 16 17 18 19

not examined

homogeneous

not examined expression: see section 01 02 03 04 24

neural retina

strong

regional

strong expression: see section 01 02 03 04 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 16 17 18 19 20 21

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 14 17

genital tubercle of male

moderate

regional

moderate expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6694
Entity Detected:	Ggt7, gamma-glutamyltransferase 7 ( MGI:1913385)
Sequence:	sense strand is shown >T6694 GGCGCTGCGCTGCTGGGGGTTAGGGCGAGGATGGCGGCTGAGAACGAGGCTAGCCAGGAGAGCGCCCTAG GCGCCTACTCACCAGTGGACTATATGAGCATCACCAGCTTCCCGAGGCTGCCGGAGGACGAGCCAGCGCC TGCGGCCCCACTGAGGGGCCGTAAGGACGAGGACGCCTTCTTGGGAGACCCGGATACTGACCCGGACTCC TTCCTCAAGTCTGCCCGGCTTCAGCGGCTGCCTTCGTCGTCATCGGAGATGGGCAGCCAGGACGGGTCAC CATTACGGGAGACTCGCAAGGACCCATTCTCAGCTGCAGCAGCAGAGTGTTCTTGCCGCCAGGATGGGCT CACGGTCATTGTCACCGCCTGCCTCACTTTTGCCACAGGTGTCACCGTGGCGCTGGTCATGCAAATCTAC TTCGGGGATCCCCAGATCTTCCAGCAGGGTGCTGTGGTGACGGATGCCTCCCATTGCACGGCGCTGGGCA TGGAGGTGCTCAGTAAACAGGGCTCTTCAGTGGATGCAGCTGTGGCCGCAGCCTTGTGTTTGGGGATTGT GGCTCCACACAGTTCTGGCCTGGGCGGTGGGGGCGTGATGTTGGTACATGATATCCGGCGGAATGAGAGC CACCTAATTGATTTCCGGGAGTCTGCACCCGGGGCCCTCAGAGAAGAGGCTCTGCAGAGATCCTGGGATA CCAAGCCTGGGCTCTTGGTGGGGGTCCCCGGAATGGTGAAAGGGTTACATGAAGCTCACCAGCTCTATGG CAGGCTGCCCTGGTCCCAAGTCCTGGCCTTCGCAGCAGCTGTGGCCCAAGATGGCTTCAACGTGACCCAT GATCTAGCTCATGCCTTGGCTGAGCAGCTGCCTCCCAATGCATCTGACCGCTTCCTGGATACTTTCCTGC CACTGGGCCACCCACCCTTGCCTGGCTCCCTGCTGCGACGGCCTGATCTCGCTGAGGTACTGGATATACT CGGCACCTCTGGCCCTGCTGCCTTCTACAATGGTGGCAACCTCACACTGGAGATGGTGGCTGAGGCTCAG CACGCAGGTGGTGTCATAACCGAGGAGGACTTCAGCAACTATAGTGCTCTCACGGAGAAGCCTGTGTGTG GAGTGTACCGAGGCCACCTGGTTCTCAGCCCCCCACCCCCACACACAGGCCCGGCCCTCATCAGTGCTCT CAACATCCTGGAGGGCTTCAACCTCACCAGCCTGGTGTCCCGTGAGCAAGCGCTTCACTGGGTAGCAGAG ACCCTGAAGATTGCATTGGCCCTGGCTAGCAGACTGGGAGACCCTGTCTATGATTCTACCATCACTGAGA GCATGGACGACATGCTCAGCAAGGTGGAGGCCGCCAACTTCCGGGGCCACATCAGTGACTCTCAGGCAGC CCCTGCCCCACTCCTGCCTGTCTATGAGCTGGATGGGGCTCCCACTGCTGCCCAGGTGCTGGTCATGGGC CCTGATGACTTCATCGTAGCCATGGTCAGCTCCCTGAACCGGCCTTTTGGAAGTGGCCTCCTCACTCCCT CCGGGATCCTTCTCAACAGCCAGATGCTAGACTTCTCCTGGCCCAACAGGACAGCCAACCACTCTGCACC CAGCCTGGAGAACTCGGTACAGCCAGGGAAGCGGCCCCTCTCTTTCCTTCTGCCCACTGTAGTCCGACCA GCAGAAGGGCTCTGTGGGACCTACCTCGCTCTGGGGGCCAATGGAGCTGCTCGAGGCCTCAGTGGCCTGA CCCAGGTTCTACTGAATGTCCTGACCTTGAACAGGAACCTGAGTGACAGCCTGGCCCGAGGACGCCTCCA CCCAGACCTGCAGTCCAACCTCCTGCAGGTTGACAGTGAGTTCACTGAGGAAGAGATTGAGTTCCTGGAA GCCAGGGGTCACCACGTGGAGAAGGTAGATGTCTTATCCTGGGTCCACGGCAGTCGGAGAACCAACACCT TCATCATCGGTGTGAAGGACCCTCGGAGCCCGGATGCAGCTGGAGCCACCATCCTGTAGAGCATTGGGCT GGGCGGGATCTCTGCCTTTGCATGTTCCCAGAGTCCCTCTTTCTCCCAGGTTTGGTCTCAGGGGGACCCC ATGGATGCCTCAGATTAGGGGCCAAAGGGGATGCTTAGCAAACCCTATCCCAGAGTAACTGGAAAATTCT CCAACTGGAGGCCTTTGGTGGGAATGATGACATCAGTGTCCATGACCAGGCAGGCAGGACCTTGAGGAGT CAGATTGTCTGTCTCCTCTCCTCTTTCAGCCATGCTATCCTTGAGCTTAGGGATGTGCTTGCAAACCCTT CTCAAGGGTCTCACTATCCCAACACCTTCAGACTGGTCTGACCTAGGCCTTGTCTTCCAGCTCCCTTTTC CTGTATCTGGCCCCATTTCTTAAGTGACTAGGATTTTTTTAAAAAATTGGCCATTATGGGGAGAGGTAAT CCTCGCCTTCCACCAGGTTGAGGCAGGGGCCTTGCATTTGAAGGTCCCCAGGCAGCCACACAGGGGTGGG GTGGGTATCAGCTCAGGGGCTTCCATTTGCAATGGGGAATTAAAGAAAGAATATTGCTTAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3602314 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3602314 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11149 same embryo
	EMAGE:11151 same embryo
	EMAGE:11147 same embryo
	EMAGE:11148 same embryo
	EMAGE:11146 same embryo
	EurExpress:euxassay_003479 same experiment
	MGI:4825050 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source