EMAGE mouse gene expression spatial database

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EMAGE:11170

Canx calnexin ( MGI:88261)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003593_01	euxassay_003593_02	euxassay_003593_03	euxassay_003593_04	euxassay_003593_05

euxassay_003593_06	euxassay_003593_07	euxassay_003593_08	euxassay_003593_09	euxassay_003593_10

euxassay_003593_11	euxassay_003593_12	euxassay_003593_13	euxassay_003593_14	euxassay_003593_15

euxassay_003593_16	euxassay_003593_17	euxassay_003593_18	euxassay_003593_19	euxassay_003593_20

euxassay_003593_21	euxassay_003593_22	euxassay_003593_23	euxassay_003593_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11170_wholemount_strong.wlz

11170_wholemount_moderate.wlz

11170_wholemount_weak.wlz

11170_wholemount_possible.wlz

11170_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11170_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6178
Entity Detected:	Canx, calnexin ( MGI:88261)
Sequence:	sense strand is shown >T6178 GGTCGGGCCGCCTCTGCCGCTGTCTCCACTGCAGCACGGGGCCGGGTGTGCGGGTGGGAGAAGGTGAGGG AGCCGCCAGTGGTCCCCGGGAGGCTCGAGATAGATCATGGAAGGGAAGTGGTTACTGTGTTTGCTGCTGG TCCTTGGAACTGCAGCTGTTGAGGCTCATGATGGACATGATGATGACGCGATTGATATTGAAGATGATCT TGATGATGTTATTGAAGAGGTAGAAGATTCAAAATCTAAATCAGATGCCAGCACTCCTCCATCTCCAAAG GTCACCTACAAAGCTCCAGTTCCAACAGGGGAGGTTTATTTTGCTGACTCCTTTGACAGAGGGTCTCTGT CAGGGTGGATTTTATCTAAAGCCAAAAAAGATGACACTGATGATGAAATTGCCAAATATGATGGAAAGTG GGAAGTAGATGAGATGAAGGAAACAAAGCTTCCAGGGGATAAAGGACTTGTACTGATGTCTCGGGCCAAG CATCATGCCATCTCTGCTAAACTGAATAAGCCCTTCCTGTTTGATACCAAGCCTCTCATTGTTCAGTATG AGGTTAATTTTCAGAATGGAATAGAATGTGGTGGTGCCTATGTGAAGCTGCTTTCCAAGACGGCAGAGCT CAGCCTGGATCAATTCCACGACAAGACTCCCTATACTATTATGTTTGGTCCAGATAAGTGTGGAGAGGAC TACAAACTGCATTTCATCTTTCGACACAAAAATCCCAAGACAGGTGTATATGAAGAAAAACATGCTAAGA GGCCAGATGCAGATCTGAAGACCTATTTCACTGACAAGAAAACGCATCTTTATACATTAATCTTGAATCC AGACAATAGTTTTGAAATATTAGTTGACCAGTCTGTTGTGAACAGTGGAAATCTGCTAAATGACATGACT CCTCCTGTAAACCCTTCACGTGAAATTGAAGACCCAGAAGACCGGAAGCCTGAAGATTGGGATGAAAGGC CCAAAATAGCAGATCCAGATGCTGTCAAGCCAGATGACTGGGATGAAGACGCCCCTTCTAAGATCCCAGA TGAAGAGGCCACCAAGCCTGAAGGCTGGCTAGACGACGAACCTGAGTATATTCCAGACCCTGATGCAGAG AAGCCAGAGGATTGGGATGAGGATATGGACGGAGAATGGGAGGCTCCTCAGATTGCCAACCCCAAGTGTG AGTCAGCCCCTGGGTGTGGTGTCTGGCAGCGACCTATGATTGACAACCCCAATTATAAGGGCAAATGGAA GCCTCCAATGATTGACAACCCTAACTACCAGGGAATCTGGAAACCAAGGAAAATACCAAATCCAGATTTC TTTGAAGACCTAGAACCTTTTAAGATGACTCCTTTCAGTGCTATTGGTTTGGAGCTCTGGTCCATGACAT CCGACATCTTTTTTGACAACTTTATCATTAGTGGTGACCGAAGAGTAGTTGATGATTGGGCCAATGATGG GTGGGGCCTGAAGAAAGCTGCTGATGGGGCTGCTGAGCCAGGTGTAGTGCTGCAGATGCTGGAGGCAGCT GAAGAGCGTCCATGGCTTTGGGTGGTCTACATTTTGACTGTAGCTTTGCCAGTGTTCCTTGTGATCCTCT TCTGCTGTTCTGGAAAGAAACAGTCCAATGCTATGGAGTACAAGAAGACGGATGCTCCCCAGCCAGATGT GAAGGATGAAGAAGGGAAGGAAGAAGAGAAGAACAAGAGGGATGAAGAGGAAGAAGAGGAGAAGCTTGAA GAGAAACAGAAGAGTGATGCTGAAGAAGATGGTGTTACTGGCAGTCAAGATGAGGAAGATAGCAAGCCTA AAGCAGAGGAGGATGAAATTTTGAACAGATCGCCAAGAAACAGAAAGCCACGAAGAGAGTGAAACAATCT TTAAGAACTTGATCTGTGATTTCCTCTCCCTCCCCTTCCCCTGCAAATGTGGTCCTAGGAGAGGGCCTGG TGTACCTTAGGTGGGAGCTCAAAACCTCAAGATGTCACCATCAACAGGTTCCAGTGAATACTAGCCTGTA ATTTTAAACATCTAGCAGTAAATAATTGCAGTTGTAATGTAAAGGACCCTGTTTCTGTAGAAAGGAAAAC ATTTAACATAATGGTTGTGAAATGTAACATGAAGCAACTAACTAGTGTTTTGTTGTTTTTAAAATTTTCC AGGTTTGTCTTTTTTTTTTTCCTTTTCCTTTTTTTTTTTTTTTTAGATACAATGATAGAATTTTTGCCAG TTTAAAATCTTGGCTTAATTTAATATATTCATTTGTTCATGCAGAAATAACACCAACATTAGGAATGCTA GGGAGAATGAATTGCCGTTTTTATAATAACTTTTTTAAGTTTGGTATTAAAGCATTCATGTGTCTGTCCT AAAATTGTAATCACGTTCAAGTGCAGTTATTTGTGGTTATAAATCTTGTTTTGTGTGCTTCCATTAGTTA ATTCCTCTTAAAGACACAAGAGCGATTGGATGGAAGCCCAAATTTATATCAAAGTTCCCTTTACATTGTA TTGAATAGACCCAAAAAGAACTAAAAGGAGACTTTGACTTGGTCATTTCACATCAGATCATAAAGTGCAG TGTTCTGTTGCCCTAGAGACTGCTCCATTCACAAGCACCTTTGAAGAGGGAAGGAGCTGGCTTCATGTAG CAGTTCATACTGTACTTCCCACATAGGAGGTCTGACAGCTCGGGTCCTCTGGAGCACAAGGCTTTTTAAG GAATGCTGGTGGTGCCTGGGTAGATAATTACATCACTTGTTCCACTGTGTTGACACTGTTTTCCTCATGG ATCTCCTCCATTCCTAGCTTTCTCTGCTATGCATTTTCTTCACAGCGCAGCTTGCGGTCCGTTGCTGAAA ATTATAAGCTCTGCATAGTGTTGGCTTTACTGTGATGACATGTTTCTTCTTTTTTAGCTGGCCCACACCT TTCTAGGGTCCAACTACAGGATAGATTACAGACTTTCCATTAGTGTCTATTTCTTTTACTCTGTGTAGAC TTTAGAAAGTCTAATCAATCCAGAGATGGGCCAATTCAGAATTGACTATAATTGAACACCTGCTAAAAGT ATTTATGGGAGGATTGACACACAGCATGAGTTATTTGACTTTTGTAGGATATTTAAAATCTTCATTTGCA GTTCATGTAACAGTTGTGTCTTAAAATTCACATAATAAAGCAGTCCTGTTCAAAAAAAAAAAATTTTTTT TTTGTGGCTTGTAGAATTTTTTAAAAGTGTATTTAGGGCTTTTTGTTTTTTGTTTGTTTTTTTGTTTTGT TTTTCATGTGGAATGCAGATGGGTGCTAGAGGAGCCTCTCCCACATCACTATAGTGTAATATTATTACAC CACACTGAAATGTATTCAGAAACAGATGTTTCAATTTCATTCTTTCTCCAGAGGTGGTCCAGTTGTACCA ATGATACTATTCCTTGCACTGAATATATATAAACACTCTTCAGTGTTTATATTGGGGAAATACTGGGAAA GAAATATATTTGTTAAGGATGAAGGCTGTATCTGTTGTCTTTATAAACACTGGTTCATTTCTTTTGAGGG TGGAATGCATTTTTCTTCCTGTTCAGTGCTTTATCACCATCTGTTGTTTGTGGTCACAGTGACCATAGCT ATGTAGCGGACGTTTCCAAATGTATAGAGTGAAATAAACAGTTACTAGCAAGAAATGAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3497769 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3497769 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11174 same embryo
	EMAGE:11172 same embryo
	EMAGE:11175 same embryo
	EMAGE:11173 same embryo
	EMAGE:11171 same embryo
	EurExpress:euxassay_003593 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source